LOCUS       40924_30705        7777 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7777)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1267..1308
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             1318..1335
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        2443..2818
                     /label=ADE2 promoter
     rep_origin      complement(5749..6337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6511..7368)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7369..7473)
                     /label=AmpR promoter
ORIGIN
        1 ggcgcgcctt aattaaggcc ggcccctgca gggggccccg agatggtaca tacttaaaag
       61 ctgccatatt gaggaacttc aaagttttat ctgtttttag aattaaaaga cgattgttgt
      121 aacaaaacgt tgtgcctaca taaactcaaa ttaatggaaa tagcctgttt tgaaaaatac
      181 accttcttaa gtactgacaa agttttgtta aatgactatc gaacaagcca tgaaatagca
      241 catttctgcc agtcactttt aacactttcc tgcttgctgg ttgactctcc tcatacaaac
      301 acccaaaagg gaaactttca gtgtggggac acttgacatc tcacatgcac cccagattaa
      361 tttccccaga cgatgcggag acaagacaaa acaacccttt gtcctgctct tttctttctc
      421 acaccgcgtg ggtgtgtgcg caggcaggca ggcaggcagc gggctgcctg ccatctctaa
      481 tcgctgctcc tcccccctgg cttcaaataa cagcctgctg ctatctgtga ccagattggg
      541 acacccccct cccctccgaa tgatccatca ccttttgtcg tactccgaca atgatccttc
      601 cctgtcatct tctggcaatc agctccttca ataattaaat caaataagca taaatagtaa
      661 aatcgcatac aaacgtcatg aaaagtttta tctctatggc caacggatag tctatctgct
      721 taattccatc cactttggga accgttctct ctttacccca gattctcaaa gctaatatct
      781 gccccttgtc tattgtcctt tctccgtgta caagcggagc ttttgcctcc catcctcttg
      841 ctttgtttcg gttatttttt ttttcttttg aaactcttgg tcaaatcaaa tcaaacaaaa
      901 ccaaaccttc tattccatca gatcaacctt gttcaacatt ctataaatcg atataaatat
      961 aaccttatcc ctcccttgtt ttttaccaat taatcaatct tcaaatttca aatattttct
     1021 acttgcttta ttactcagta ttaacatttg tttaaaccaa ctataacttt taactggctt
     1081 tagaagtttt atttaacatc agtttcaatt tacatcttta tttattaacg aaatctttac
     1141 gaattaactc aatcaaaact tttacgaaaa aaaaatctta ctattaagag ctcgagagga
     1201 attcacgtgg cccagccggc cgtggatcca cgcgtcgtcg acccgcggcg gccgccagct
     1261 tacgtaggta agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat
     1321 catcaccatc accattgatc tagtatacaa ttctagggct gcctgtttgg atatttttat
     1381 aatttttgag agtttgccaa ctaatgtttt tctcttctat gatatttatc atgtagttgg
     1441 atatcttttt ttctttgaaa agtttttatt tagttctttc tctctttttt ataatgtttt
     1501 cagtttcttt cctttatatt gaaaatacga tgttctttgt ctttcttttt taaatcctca
     1561 attataatgt gtaccgtttg atcaacttgt aaaattgttt gtagtcctgt aatcttaatt
     1621 gacgattggt tgatagttta ccccagtata ttgtgcggct gagatattac ccgattcgga
     1681 agattgattt tttcaacaga cataaaccca taggagattt agtacagcga catatgctaa
     1741 ttgcctgata atgctcacta acattttctc cttttaatct ggtatatgca tacaggtatg
     1801 ttgatatggc cttgttgatc aaagctacga aagtgtagac tatttcccaa acagccccca
     1861 accaccacct cacacgcgca cactgcgaag tgaaagcgct cccttatttt cttctccgct
     1921 ggttcaaaac aaaaaagaat ctaatctgta gacacacgcc ccgtaaaacc caatcttcat
     1981 catcgtaatt ttcagtgacg gacgatcgat caaccatata gtttgttagt gactgagctt
     2041 cagtagttga cctcttgaag aaataacgtc ttgaacaaca tactttatac ctaccaacat
     2101 tcgccatcaa caacagtgtg acactgacat aactttacat ccattcatcg acaaaaaaat
     2161 gtctctagtt cggattcatg cttatgggtt agctatttcg cccaatgtgt ccatctgaca
     2221 ttactatttt gcattttaat ttaattagaa cttgactagc gcactaccag catatcatct
     2281 catttccgta aataccaaat gtattatata ttgaaagctt ttgaccaggt tattataaaa
     2341 gaaacttcat gctcgaaaaa gatcatttcg aaaagttgcc tagtttcatg aaattttaaa
     2401 gcagtttata taaattttac cttttgatgc ggaattgact ttttcttgaa taatacataa
     2461 cttttcttaa aagaatcaaa gacagataaa atttaagaga tattaaatat tagtgagaag
     2521 ccgagaattt tgtaacacca acataacact gacatcttta acaactttta attatgatac
     2581 atttcttacg tcatgattga ttattacagc tatgctgaca aatgactctt gttgcatggc
     2641 tacgaaccgg gtaatactaa gtgattgact cttgctgacc ttttattaag aactaaatgg
     2701 acaatattat ggagcatttc atgtataaat tggtgcgtaa aatcgttgga tctctcttct
     2761 aagtacatcc tactataaca atcaagaaaa acaagaaaat cggacaaaac aatcaagtat
     2821 ggattctaga acagttggta tattaggagg gggacaattg ggacgtatga ttgttgaggc
     2881 agcaaacagg ctcaacatta agacggtaat actagatgct gaaaattctc ctgccaaaca
     2941 aataagcaac tccaatgacc acgttaatgg ctccttttcc aatcctcttg atatcgaaaa
     3001 actagctgaa aaatgtgatg tgctaacgat tgagattgag catgttgatg ttcctacact
     3061 aaagaatctt caagtaaaac atcccaaatt aaaaatttac ccttctccag aaacaatcag
     3121 attgatacaa gacaaatata ttcaaaaaga gcatttaatc aaaaatggta tagcagttac
     3181 ccaaagtgtt cctgtggaac aagccagtga gacgtcccta ttgaatgttg gaagagattt
     3241 gggttttcca ttcgtcttga agtcgaggac tttggcatac gatggaagag gtaacttcgt
     3301 tgtaaagaat aaggaaatga ttccggaagc tttggaagta ctgaaggatc gtcctttgtc
     3361 gccgaaaaat gggcaccatt tactaaagaa ttagcagtca tgattgtgag atctgttaac
     3421 ggtttagtgt tttcttaccc aattgtagag actatccaca aggacaatat ttgtgactta
     3481 tgttatgcgc ctgctagagt tccggactcc gttcaactta aggcgaagtt gttggcagaa
     3541 aatgcaatca aatcttttcc cggttgtggt atatttggtg tggaaatgtt ctatttagaa
     3601 acaggggaat tgcttattaa cgaaattgcc ccaaggcctc acaactctgg acattatacc
     3661 attgatgctt gcgtcacttc tcaatttgaa gctcatttga gatcaatatt ggatttgcca
     3721 atgccaaaga atttcacatc tttctccacc attacaacga acgccattat gctaaatgtt
     3781 cttggagaca aacatacaaa agataaagag ctagaaactt gcgaaagagc attggcgact
     3841 ccaggttcct cagtgtactt atatggaaaa gagtctagac ctaacagaaa agtaggtcac
     3901 ataaatatta ttgcctccag tatggcggaa tgtgaacaaa ggctgaacta cattacaggt
     3961 agaactgata ttccaatcaa aatctctgtc gctcaaaagt tggacttgga agcaatggtc
     4021 aaaccattgg ttggaatcat catgggatca gactctgact tgccggtaat gtctgccgca
     4081 tgtgcggttt taaaagattt tggcgttcca tttgaagtga caatagtctc tgctcataga
     4141 actccacata ggatgtcagc atatgctatt tccgcaagca agcgtggaat taaaacaatt
     4201 atcgctggag ctggtggggc tgctcacttg ccaggtatgg tggctgcaat gacaccactt
     4261 cctgtcatcg gtgtgcccgt aaaaggttct tgtctagatg gagtagattc tttacattca
     4321 attgtgcaaa tgcctagagg tgttccagta gctaccgtcg ctattaataa tagtacgaac
     4381 gctgcgctgt tggctgtcag actgcttggc gcttatgatt caagttatac aacgaaaatg
     4441 gaacagtttt tattaaagca agaagaagaa gttcttgtca aagcacaaaa gttagaaact
     4501 gtcggttacg aagcttatct agaaaacaag taatatataa gtttattgat atacttgtac
     4561 agcaaataat tataaaatga tatacctatt ttttaggctt tgttatgatt acatcaaatg
     4621 tggacttcat acatagaaat caacgcttac aggtgtcctt ttttaagaat ttcatacata
     4681 agatctacta gaatcaatgt taaacctgtt atcattagaa cagccatctc cagaaggttc
     4741 tatccaagtg aaaactttgg tcttcaagcc aaagactgct aaaacacaaa ccccagtccc
     4801 aattattgtt gttgctgaaa cttctacttc tactccgggt ggtgttatag ccaaagcttc
     4861 gggcactaaa gagccaagat tagcaaacga tgaattgacc aaccaattct ttggttctcc
     4921 tgctaaagag ttttctattg ccagcttatc aaacgaacac aaagacggta aagtcaagat
     4981 cattattgat gctgggatca aagatgatga ctcgactaat tacgttattt ccaacactgt
     5041 tgctaagtta gttttaaccg gtaagcaatt attaggtttc ttatccaact acgaaccaac
     5101 catcgttgat tttactgctg aagctgcaac cgctgcccca actccagttg ttaagaaaga
     5161 acctgctgca tctggtaagc ttgatgatgc caaattaatt ggtgttactg ctgacaagga
     5221 aaaagatttc tccaactggt actcccaaat tctaatgaag ggtgaaatgt tagactacta
     5281 cgatgtctct ggttgttaca ttatgagacc agcttcttac acaatttggg aaaagattca
     5341 atcttggttc gattcgaaga ttaaagaaga tggtgttact aatgcttatt tcccaatgtt
     5401 tgtctcatcc aaggttttag aaaaggaaaa ggaccacatc gatggtttcg ctcctgaagt
     5461 tgcttgggtt accagagctg gtaaatctga attagaagaa ccaattgcta ttagaccaac
     5521 ttctgaaact gttatgggta ccctgcaggg gccggcctta attaaggcgc gcccttcctc
     5581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     5641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     5701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     5761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     5821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     5881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     5941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     6661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     6721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     6781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     6841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     6901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     6961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgt
     7501 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gaaattgtaa
     7561 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     7621 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     7681 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     7741 ggcgaaaaac cgtctatcaa gggcgatggc ccactac
//
