LOCUS       V004760                 3858 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004760
VERSION     V004760
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3858)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3858)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..632
                     /label="SR-alpha promoter"
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human
                     T-lymphotrophic virus 1"
     intron          662..755
                     /label="SV40 intron"
                     /note="modified SV40 intron with splice donor and acceptor
                     sites"
     promoter        800..818
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             861..1676
                     /gene="IL2RA"
                     /label="Interleukin-2 receptor subunit alpha"
                     /note="Interleukin-2 receptor subunit alpha from Homo
                     sapiens. Accession#: P01589"
     polyA_signal    1763..1897
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2107..2695)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2869..3726)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3727..3831)
                     /label="AmpR promoter"
ORIGIN
        1 aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
       61 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
      181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
      241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
      301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat
      361 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc
      421 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc
      481 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta
      541 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt
      601 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact
      661 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag
      721 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc
      781 tctaaaagct gcggaattct aatacgactc actataggga gtcgactcag atcttcgata
      841 aacttaagct tggtaccacc atggattcat acctgctgat gtggggactg ctcacgttca
      901 tcatggtgcc tggctgccag gcagagctct gtgacgatga cccgccagag atcccacacg
      961 ccacattcaa agccatggcc tacaaggaag gaaccatgtt gaactgtgaa tgcaagagag
     1021 gtttccgcag aataaaaagc gggtcactct atatgctctg tacaggaaac tctagccact
     1081 cgtcctggga caaccaatgt caatgcacaa gctctgccac tcggaacaca acgaaacaag
     1141 tgacacctca acctgaagaa cagaaagaaa ggaaaaccac agaaatgcaa agtccaatgc
     1201 agccagtgga ccaagcgagc cttccaggtc actgcaggga acctccacca tgggaaaatg
     1261 aagccacaga gagaatttat catttcgtgg tggggcagat ggtttattat cagtgcgtcc
     1321 agggatacag ggctctacac agaggtcctg ctgagagcgt ctgcaaaatg acccacggga
     1381 agacaaggtg gacccagccc cagctcatat gcacaggtga aatggagacc agtcagtttc
     1441 caggtgaaga gaagcctcag gcaagccccg aaggccgtcc tgagagtgag acttcctgcc
     1501 tcgtcacaac aacagatttt caaatacaga cagaaatggc tgcaaccatg gagacgtcca
     1561 tatttacaac agagtaccag gtagcagtgg ccggctgtgt tttcctgctg atcagcgtcc
     1621 tcctcctgag tgggctcacc tggcagcgga gacagaggaa gagtagaaga acaatctagg
     1681 cggccgctcg agtctctaga gaaaaaacct cccacacctc cccctgaacc tgaaacataa
     1741 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     1801 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     1861 gtccaaactc atcaatgtat cttatcatgt ctggatcccc gggtaccgag ctcgaattaa
     1921 ttcctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1981 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2041 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2101 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2161 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     2221 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     2281 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     2341 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2401 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2461 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2521 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     2581 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2641 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2701 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2761 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2821 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2881 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     2941 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3001 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3061 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     3121 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     3181 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     3241 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     3301 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     3361 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     3421 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     3481 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     3541 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     3601 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     3661 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     3721 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     3781 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     3841 ccgaaaagtg ccacctgc
//
