LOCUS       40924_30635        4154 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant expression vector pMESHI, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ferrando A, Koncz-Kalman Z, Farras R, Tiburcio A, Schell J, Koncz C.
  TITLE     Detection of in vivo protein interactions between Snf1-related 
            kinase subunits with intron-tagged epitope-labelling in plants cells
  JOURNAL   Nucleic Acids Res. 29 (17), 3685-3693 (2001)
  PUBMED    11522840
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Berendzen KW, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant 
            Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany
REFERENCE   3  (bases 1 to 4154)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4154)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001"; volume: "29"; issue: "17"; pages: 
            "3685-3693"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2004) Development, Max-Planck Institute for Plant Breeding 
            Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4154
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..18
                     /note="Tth111I, NotI, XhoI, KpnI; MCS promoter"
     promoter        409..754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    759..793
                     /note="HindIII, NcoI, SmaI, XbaI, BglII; MCS upstream"
     misc_feature    794..808
                     /label=N-term c-myc epitope
                     /note="N-term c-myc epitope"
     intron          809..997
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     misc_feature    998..1012
                     /label=C-term c-myc epitope
                     /note="C-term c-myc epitope"
     misc_feature    1013..1038
                     /note="SalI, BamHI, PstI, EcoRI; MCS downstream"
     regulatory      1039..1291
                     /note="polyadenylation signal from nopaline synthase gene
                     of the Agrobacterium Ti plasmid; NOS terminator"
                     /regulatory_class="terminator"
     promoter        complement(1323..1341)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1371..1387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1395..1411)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1419..1449)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1464..1485)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1773..2361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2535..3392)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3393..3497)
                     /label=AmpR promoter
     rep_origin      3524..3979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4120..4136
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcggccgctc gaggtacccc tactccaaaa atgtcaaaga tacagtctca ntaagnacca
       61 aagggctatt gagacttttc aacaaagggt aatttcggga aacctcctcg gattccattg
      121 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
      181 ccatcattgc gataaaggaa aggctatcat tcaagatgcc tctgccgaca gtggtcccaa
      241 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      301 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccaccc
      361 ctactccaaa aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca
      421 acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      481 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
      661 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacagcccaa gcttccacca tggcgcccgg
      781 gtctagaaga tctgagcaaa agttgattgt aagtttctgc ttctaccttt gatatatata
      841 taataattat cattaattag tagtaatata atattttaaa tatttttttc aaaataaaag
      901 aatgtagtat atagcaattg cttttctgta gtttataagt gtgtatattt caatttataa
      961 cttttctaat atatgaccaa aatttgttga tgtgcagtct gaggaggatc ttgtcgacgg
     1021 atccactgca gtgaattctc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     1081 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     1141 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     1201 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     1261 gcgcgcggtg tcatctgtcg agggggggcc cggtaccgag ctcgcggccg ctcgacgagc
     1321 tccctatagt gagtcgtatt anaggccgac ttggccaaat tcgtaatcat gtcatagctg
     1381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     1441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1561 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     2161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     2221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     2281 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     2341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     2401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     2461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     2521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     2581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     2641 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     2701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     2761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     2821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     2881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     2941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     3001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     3061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     3121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     3181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     3241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     3301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     3361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     3421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     3481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc
     3541 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3601 ctagggcccg gtcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3661 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3721 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3781 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3841 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     3901 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3961 atattaacgc ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     4021 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     4081 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca
     4141 agctgacttg gtca
//
