LOCUS       40924_30625       10440 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10440)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(948..1082)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    complement(1761..1809)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(1877..2899)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(2939..3084)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     rep_origin      complement(3701..4289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4463..5320)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5321..5425)
                     /label=AmpR promoter
     CDS             5946..7868
                     /codon_start=1
                     /label=EBNA1
                     /note="Epstein-Barr nuclear antigen 1, also known as
                     EBNA-1"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      8177..9966
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tctagaggtc gaccaattct catgtttgac agcttatcat cgcagatctg agcttgtggc
       61 ttcttctcct tactcttcct cctccttggt gtctgtatgt tagagggccg ttagcatctg
      121 catctgctgg ggcctggtcg cattcacctg ctctgccact cactggctgt gtgactctgc
      181 agaaattaac ttctctggac ctggcagttt ctcctctcta caatgagaat actggagagt
      241 ccttatctta tgggttgcta cagaattaag tgacatctca cacacaacac acttcctaca
      301 gtccctgtta cacgctaaaa gtactcaact agcttcggat acgtcatcag caaccacccc
      361 acgggttact gtgatgctgc acaattatta agccctggct gctacagagt tgtaacctgt
      421 ctgcacttcc aaccggcgcc gcaagcagca ttcccagtcc cgtttcaccc gcgcgctaac
      481 ggctcagttc gagtacagga caggagggag gggagctgtg cacacggcgg aggcgcacgg
      541 cgtgggcacc cagcacccgg tacactgtgt cctcccgctg cacccagccc cttcagcccg
      601 aggcgtcccc gaggcgcaac tgggccgcct tcaggggaac tgaccgcccg cggcccgtgt
      661 gcagagccgg gtgcgcccgg cccagtgcgc gcggccgggt gtttcgcttg gagccgcaag
      721 tgacttctag cgcggggcgt gtgcagcacg gccggggcgg ggcttttgca ctcgtcccgg
      781 ctctttctag ctataaacac tgcttgccgc gctgcactcc accacgcctc ctccaagtcc
      841 cagcgaaccc gcgtgcaacc tgtcccgact ctagccgcct cttcagctca cggagggtac
      901 cagctgctag caagcttgct agcggccgct cgaggccggc aaggccggat ccagacatga
      961 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     1021 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     1081 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg gggaggtttt
     1141 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatccgg ctgcctcgcg
     1201 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     1261 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     1321 gggtgtcggg gcgcagccat gaccggtcga cggcactggg cgccagaaat ccgcgcggtg
     1381 gtttttgggg gtcgggggtg tttggcagcc acagacgccc ggtgttcgtg tcgcgccagt
     1441 acatgcggtc catgcccagg ccatccaaaa accatgggtc tgtctgctca gtccagtcgt
     1501 ggaccagacc ccacgcaacg cccaaaataa taacccccac gaaccataaa ccattcccca
     1561 tgggggaccc cgtccctaac ccacggggcc agtggctatg gcagggcctg ccgccccgac
     1621 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
     1681 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
     1741 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     1801 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     1861 tcccccatct cccctattcc tttgccctcg gacgagtgct ggggcgtcgg tttccactat
     1921 cggcgagtac ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg
     1981 tacgcccgac agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag
     2041 ctgcatcatc gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga
     2101 gcatatacgc ccggagccgc ggcgatcctg caagctccgg atgcctccgc tcgaagtagc
     2161 gcgtctgctg ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt
     2221 attgggaatc cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt
     2281 ccgtcaggac attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt
     2341 cctcggccca aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt
     2401 ccatcacagt ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc
     2461 atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa
     2521 gatcggccgc agcgatcgca tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt
     2581 cggtttcagg caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca
     2641 ggctctcgct gaattcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa
     2701 agtgccgata aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga
     2761 catatccacg ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct
     2821 gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg
     2881 tgagttcagg ctttttcata tctcattgcc cgggatctgc ggcacgctgt tgacgctgtt
     2941 aagcgggtcg ctgcagggtc gctcggtgtt cgaggccaca cgcgtcacct taatatgcga
     3001 agtggacctg ggaccgcgcc gccccgactg catctgcgtg ttcgaattcg ccaatgacaa
     3061 gacgctgggc ggggtttgtg tcatcataga actaaagaca tgcaaatata tttcttccgg
     3121 ggacaccgcc agcaaacgcg agcaacgggc cacggggatg aagcagggca tggcggccga
     3181 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     3241 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     3301 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac
     3361 ttcgatcact ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa
     3421 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg
     3481 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga
     3541 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
     3601 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcagc
     3661 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3721 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3781 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3841 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     3901 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     3961 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4021 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4081 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4141 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4201 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4261 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4321 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4381 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4441 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     4501 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     4561 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     4621 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     4681 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     4741 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     4801 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     4861 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     4921 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     4981 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     5041 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     5101 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     5161 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     5221 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     5281 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     5341 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     5401 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     5461 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     5521 ttcaagaatt ctcatgtttg acagcttatc atcgataagc tgatcctcac aggccgcacc
     5581 cagcttttct tccgttgccc cagtagcatc tctgtctggt gaccttgaag aggaagagga
     5641 ggggtcccga gaatccccat ccctaccgtc cagcaaaaag ggggacgagg aatttgaggc
     5701 ctggcttgag gctcaggacg caaatcttga ggatgttcag cgggagtttt ccgggctgcg
     5761 agtaattggt gatgaggacg aggatggttc ggaggatggg gaattttcag acctggatct
     5821 gtctgacagc gaccatgaag gggatgaggg tgggggggct gttggagggg gcaggagtct
     5881 gcactccctg tattcactga gcgtcgtcta ataaagatgt ctattgatct cttttagtgt
     5941 gaatcatgtc tgacgagggg ccaggtacag gacctggaaa tggcctagga gagaagggag
     6001 acacatctgg accagaaggc tccggcggca gtggacctca aagaagaggg ggtgataacc
     6061 atggacgagg acggggaaga ggacgaggac gaggaggcgg aagaccagga gccccgggcg
     6121 gctcaggatc agggccaaga catagagatg gtgtccggag accccaaaaa cgtccaagtt
     6181 gcattggctg caaagggacc cacggtggaa caggagcagg agcaggagcg ggaggggcag
     6241 gagcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca ggaggggcag
     6301 gaggggcagg aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg
     6361 caggaggggc aggagcagga ggaggggcag gagcaggagg aggggcagga ggggcaggag
     6421 caggaggagg ggcaggaggg gcaggagggg caggagcagg aggaggggca ggagcaggag
     6481 gaggggcagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg gcaggagcag
     6541 gaggaggggc aggagcagga ggggcaggag gggcaggagg ggcaggagca ggaggggcag
     6601 gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggggca ggagcaggag
     6661 gggcaggagc aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggggcag
     6721 gaggggcagg agcaggaggg gcaggagggg caggagcagg aggaggggca ggaggggcag
     6781 gagcaggagg aggggcagga ggggcaggag caggaggggc aggaggggca ggagcaggag
     6841 gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggagga ggggcaggag
     6901 caggaggggc aggagcagga ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc
     6961 gaggaggtag tggaggccgg ggtcgaggag gtagtggagg ccgccggggt agaggacgtg
     7021 aaagagccag ggggggaagt cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa
     7081 agaggcccag gagtcccagt agtcagtcat catcatccgg gtctccaccg cgcaggcccc
     7141 ctccaggtag aaggccattt ttccaccctg taggggaagc cgattatttt gaataccacc
     7201 aagaaggtgg cccagatggt gagcctgacg tgcccccggg agcgatagag cagggccccg
     7261 cagatgaccc aggagaaggc ccaagcactg gaccccgggg tcagggtgat ggaggcaggc
     7321 gcaaaaaagg agggtggttt ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg
     7381 agaacattgc agaaggttta agagctctcc tggctaggag tcacgtagaa aggactaccg
     7441 acgaaggaac ttgggtcgcc ggtgtgttcg tatatggagg tagtaagacc tccctttaca
     7501 acctaaggcg aggaactgcc cttgctattc cacaatgtcg tcttacacca ttgagtcgtc
     7561 tcccctttgg aatggcccct ggacccggcc cacaacctgg cccgctaagg gagtccattg
     7621 tctgttattt catggtcttt ttacaaactc atatatttgc tgaggttttg aaggatgcga
     7681 ttaaggacct tgttatgaca aagcccgctc ctacctgcaa tatcagggtg actgtgtgca
     7741 gctttgacga tggagtagat ttgcctccct ggtttccacc tatggtggaa ggggctgccg
     7801 cggagggtga tgacggagat gacggagatg aaggaggtga tggagatgag ggtgaggaag
     7861 ggcaggagtg atgtaacttg ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc
     7921 cccgcactaa agaataaatc cccagtagac atcatgcgtg ctgttggtgt atttctggcc
     7981 atctgtcttg tcaccatttt cgtcctccca acatggggca attgggcata cccatgttgt
     8041 cacgtcactc agctccgcgc tcaacacctt ctcgcgttgg aaaacattag cgacatttac
     8101 ctggtgagca atcagacatg cgacggcttt agcctggcct ccttaaattc acctaagaat
     8161 gggagcaacc agcatgcagg aaaaggacaa gcagcgaaaa ttcacgcccc cttgggaggt
     8221 ggcggcatat gcaaaggata gcactcccac tctactactg ggtatcatat gctgactgta
     8281 tatgcatgag gatagcatat gctacccgga tacagattag gatagcatat actacccaga
     8341 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     8401 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     8461 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctacccaga
     8521 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccagatata
     8581 aattaggata gcatatacta ccctaatctc tattaggata gcatatgcta cccggataca
     8641 gattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     8701 gattaggata gcctatgcta cccagatata aattaggata gcatatacta cccagatata
     8761 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     8821 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca tggcaacatt
     8881 agcccaccgt gctctcagcg acctcgtgaa tatgaggacc aacaaccctg tgcttggcgc
     8941 tcaggcgcaa gtgtgtgtaa tttgtcctcc agatcgcagc aatcgcgccc ctatcttggc
     9001 ccgcccacct acttatgcag gtattccccg gggtgccatt agtggttttg tgggcaagtg
     9061 gtttgaccgc agtggttagc ggggttacaa tcagccaagt tattacaccc ttattttaca
     9121 gtccaaaacc gcagggcggc gtgtgggggc tgacgcgtgc ccccactcca caatttcaaa
     9181 aaaaagagtg gccacttgtc tttgtttatg ggccccattg gcgtggagcc ccgtttaatt
     9241 ttcgggggtg ttagagacaa ccagtggagt ccgctgctgt cggcgtccac tctctttccc
     9301 cttgttacaa atagagtgta acaacatggt tcacctgtct tggtccctgc ctgggacaca
     9361 tcttaataac cccagtatca tattgcacta ggattatgtg ttgcccatag ccataaattc
     9421 gtgtgagatg gacatccagt ctttacggct tgtccccacc ccatggattt ctattgttaa
     9481 agatattcag aatgtttcat tcctacacta gtatttattg cccaaggggt ttgtgagggt
     9541 tatattggtg tcatagcaca atgccaccac tgaacccccc gtccaaattt tattctgggg
     9601 gcgtcacctg aaaccttgtt ttcgagcacc tcacatacac cttactgttc acaactcagc
     9661 agttattcta ttagctaaac gaaggagaat gaagaagcag gcgaagattc aggagagttc
     9721 actgcccgct ccttgatctt cagccactgc ccttgtgact aaaatggttc actaccctcg
     9781 tggaatcctg accccatgta aataaaaccg tgacagctca tggggtggga gatatcgctg
     9841 ttccttagga cccttttact aaccctaatt cgatagcata tgcttcccgt tgggtaacat
     9901 atgctattga attagggtta gtctggatag tatatactac tacccgggaa gcatatgcta
     9961 cccgtttagg gttaacaagg gggccttata aacactattg ctaatgccct cttgagggtc
    10021 cgcttatcgg tagctacaca ggcccctctg attgacgttg gtgtagcctc ccgtagtctt
    10081 cctgggcccc tgggaggtac atgtccccca gcattggtgt aagagcttca gccaagagtt
    10141 acacataaag gcaatgttgt gttgcagtcc acagactgca aagtctgctc caggatgaaa
    10201 gccactcagt gttggcaaat gtgcacatcc atttataagg atgtcaacta cagtcagaga
    10261 acccctttgt gtttggtccc cccccgtgtc acatgtggaa cagggcccag ttggcaagtt
    10321 gtaccaacca actgaaggga ttacatgcac tgccccgaat acaaaacaaa agcgctcctc
    10381 gtaccagcga agaaggggca gagatgccgt agtcaggttt agttcgtccg gcggcggggc
//
