LOCUS       40924_30610        7781 bp DNA     circular SYN 18-DEC-2018
DEFINITION  UAS-less reporter vector pMELbeta, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7781)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Zero background yeast reporter plasmids
  JOURNAL   Gene 247 (1-2), 53-61 (2000)
  PUBMED    10773444
REFERENCE   2  (bases 1 to 7781)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2003) Institute for Microbiology, Frankfurt 
            University, Marie Curie Strasse 9, Frankfurt 60439, Germany
REFERENCE   3  (bases 1 to 7781)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 247 
            (1-2), 53-61 (2000)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-SEP-2003) Institute for Microbiology, Frankfurt University, 
            Marie Curie Strasse 9, Frankfurt 60439, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             365..3373
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VSRLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRFAWFPA
                     PEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENPTGCYS
                     LTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEFDLSAFLRAGENR
                     LAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATRFNDDFSRAVLEA
                     EVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLRLNVENPKLW
                     SAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEHH
                     PLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG
                     MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSV
                     DPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGETRPLILCE
                     YAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNPWSAYGGDFGDTP
                     NDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYLFRHSDNELLHWM
                     VALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQPNATAWSEAGHI
                     SAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQSGFLSQMWIGDK
                     KQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLAD
                     AVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQV
                     AERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQ
                     WRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAQFQL
                     SAGRYHYQLVWCQK"
     CDS             complement(3574..4374)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(4375..4595)
                     /label=URA3 promoter
     misc_feature    complement(4854..5357)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        5394..5498
                     /label=AmpR promoter
     CDS             5499..6356
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6530..7118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tcgagaagaa gaaaggaaga catgtattac attatccaac aaaaaatggt tcttgacgtc
       61 tacaaatcaa gaatcttaaa gacattgaac gaagtagctg aataaaaatt atgaaaacta
      121 taaaaactat aaaaactgta cttaagtcct caataaaaca taaacttctt actgtataag
      181 gttttcgata atttcttact tgattctagg agagcaacgg taataaaagc aaacgacgat
      241 gaacggatcc ggagcttggc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
      301 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      361 acaggtttcc cgacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
      421 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
      481 ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
      541 tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
      601 ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
      661 gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
      721 gcgaattatt tttgatggcg ttaactcggc gtttcatctg tggtgcaacg ggcgctgggt
      781 cggttacggc caggacagtc gtttgccgtc tgaatttgac ctgagcgcat ttttacgcgc
      841 cggagaaaac cgcctcgcgg tgatggtgct gcgttggagt gacggcagtt atctggaaga
      901 tcaggatatg tggcggatga gcggcatttt ccgtgacgtc tcgttgctgc ataaaccgac
      961 tacacaaatc agcgatttcc atgttgccac tcgctttaat gatgatttca gccgcgctgt
     1021 actggaggct gaagttcaga tgtgcggcga gttgcgtgac tacctacggg taacagtttc
     1081 tttatggcag ggtgaaacgc aggtcgccag cggcaccgcg cctttcggcg gtgaaattat
     1141 cgatgagcgt ggtggttatg ccgatcgcgt cacactacgt ctgaacgtcg aaaacccgaa
     1201 actgtggagc gccgaaatcc cgaatctcta tcgtgcggtg gttgaactgc acaccgccga
     1261 cggcacgctg attgaagcag aagcctgcga tgtcggtttc cgcgaggtgc ggattgaaaa
     1321 tggtctgctg ctgctgaacg gcaagccgtt gctgattcga ggcgttaacc gtcacgagca
     1381 tcatcctctg catggtcagg tcatggatga gcagacgatg gtgcaggata tcctgctgat
     1441 gaagcagaac aactttaacg ccgtgcgctg ttcgcattat ccgaaccatc cgctgtggta
     1501 cacgctgtgc gaccgctacg gcctgtatgt ggtggatgaa gccaatattg aaacccacgg
     1561 catggtgcca atgaatcgtc tgaccgatga tccgcgctgg ctaccggcga tgagcgaacg
     1621 cgtaacgcga atggtgcagc gcgatcgtaa tcacccgagt gtgatcatct ggtcgctggg
     1681 gaatgaatca ggccacggcg ctaatcacga cgcgctgtat cgctggatca aatctgtcga
     1741 tccttcccgc ccggtgcagt atgaaggcgg cggagccgac accacggcca ccgatattat
     1801 ttgcccgatg tacgcgcgcg tggatgaaga ccagcccttc ccggctgtgc cgaaatggtc
     1861 catcaaaaaa tggctttcgc tacctggaga gacgcgcccg ctgatccttt gcgaatacgc
     1921 ccacgcgatg ggtaacagtc ttggcggttt cgctaaatac tggcaggcgt ttcgtcagta
     1981 tccccgttta cagggcggct tcgtctggga ctgggtggat cagtcgctga ttaaatatga
     2041 tgaaaacggc aacccgtggt cggcttacgg cggtgatttt ggcgatacgc cgaacgatcg
     2101 ccagttctgt atgaacggtc tggtctttgc cgaccgcacg ccgcatccag cgctgacgga
     2161 agcaaaacac cagcagcagt ttttccagtt ccgtttatcc gggcaaacca tcgaagtgac
     2221 cagcgaatac ctgttccgtc atagcgataa cgagctcctg cactggatgg tggcgctgga
     2281 tggtaagccg ctggcaagcg gtgaagtgcc tctggatgtc gctccacaag gtaaacagtt
     2341 gattgaactg cctgaactac cgcagccgga gagcgccggg caactctggc tcacagtacg
     2401 cgtagtgcaa ccgaacgcga ccgcatggtc agaagccggg cacatcagcg cctggcagca
     2461 gtggcgtctg gcggaaaacc tcagtgtgac gctccccgcc gcgtcccacg ccatcccgca
     2521 tctgaccacc agcgaaatgg atttttgcat cgagctgggt aataagcgtt ggcaatttaa
     2581 ccgccagtca ggctttcttt cacagatgtg gattggcgat aaaaaacaac tgctgacgcc
     2641 gctgcgcgat cagttcaccc gtgcaccgct ggataacgac attggcgtaa gtgaagcgac
     2701 ccgcattgac cctaacgcct gggtcgaacg ctggaaggcg gcgggccatt accaggccga
     2761 agcagcgttg ttgcagtgca cggcagatac acttgctgat gcggtgctga ttacgaccgc
     2821 tcacgcgtgg cagcatcagg ggaaaacctt atttatcagc cggaaaacct accggattga
     2881 tggtagtggt caaatggcga ttaccgttga tgttgaagtg gcgagcgata caccgcatcc
     2941 ggcgcggatt ggcctgaact gccagctggc gcaggtagca gagcgggtaa actggctcgg
     3001 attagggccg caagaaaact atcccgaccg ccttactgcc gcctgttttg accgctggga
     3061 tctgccattg tcagacatgt ataccccgta cgtcttcccg agcgaaaacg gtctgcgctg
     3121 cgggacgcgc gaattgaatt atggcccaca ccagtggcgc ggcgacttcc agttcaacat
     3181 cagccgctac agtcaacagc aactgatgga aaccagccat cgccatctgc tgcacgcgga
     3241 agaaggcaca tggctgaata tcgacggttt ccatatgggg attggtggcg acgactcctg
     3301 gagcccgtca gtatcggcgc aattccagct gagcgccggt cgctaccatt accagttggt
     3361 ctggtgtcaa aaataataat aaccgggcag gccatgtctg cccgtatttc gcgtaaggaa
     3421 atccattatg tactatttcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     3481 tcacaccgca tagggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag
     3541 tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata
     3601 tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc
     3661 aaatagtcct cttccaacaa taataatgtc agatcctgta gagaccacat catccacggt
     3721 tctatactgt tgacccaatg cgtctccctt gtcatctaaa cccacaccgg gtgtcataat
     3781 caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac
     3841 aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag
     3901 ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc
     3961 ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt
     4021 aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt
     4081 accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc
     4141 ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt
     4201 tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg
     4261 aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc
     4321 agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta
     4381 tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca
     4441 tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg
     4501 ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa
     4561 aaaagaataa aaaaaaaatg atgaattgaa ttgaaaagcg tggtgcactc tcagtacaat
     4621 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     4681 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     4741 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     4801 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttagg acggatcgct
     4861 tgcctgtaac ttacacgcgc ctcgtatctt ttaatgatgg aataatttgg gaatttactc
     4921 tgtgtttatt tatttttatg ttttgtattt ggattttaga aagtaaataa agaaggtaga
     4981 agagttacgg aatgaagaaa aaaaaataaa caaaggttta aaaaatttca acaaaaagcg
     5041 tactttacat atatatttat tagacaagaa aagcagatta aatagatata cattcgatta
     5101 acgataagta aaatgtaaaa tcacaggatt ttcgtgtgtg gtcttctaca cagacaagat
     5161 gaaacaattc ggcattaata cctgagagca ggaagagcaa gataaaaggt agtatttgtt
     5221 ggcgatcccc ctagagtctt ttacatcttc ggaaaacaaa aactattttt tctttaattt
     5281 ctttttttac tttctatttt taatttatat atttatatta aaaaatttaa attataatta
     5341 tttttatagc acgtgatgaa aaggacccag gtggcacttt tcggggaaat gtgcgcggaa
     5401 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5461 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5521 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5581 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5641 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5701 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5761 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5821 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5881 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5941 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     6001 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6061 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6121 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6181 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6241 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6301 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6361 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6421 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6481 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6541 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6601 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6661 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6721 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6781 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6841 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6901 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6961 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7021 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7081 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7141 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     7201 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     7261 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     7321 ctctccccgc gcgttggccg attcattccc gaattgggaa gctttctttt ttactttaat
     7381 attatgtata ctgaaaaatg cacgtgatga taagactttg gaaatttgtg taaaaccccc
     7441 attttttttt gctgctgcta ttgccaaagn caacaagtct tcaggagaca tcaacactaa
     7501 gtttctaccc cgtcttcccc tagaattctt tctgtacgct cagggtgggc ctttaaagga
     7561 tagcacccta ccgaagtcga cttctaagta aacaccatta ctaggagatg actaaatctg
     7621 gaaaaccaca tggtggtctg aatgcgtcta gtctctgcca taaacataac atgtttgttt
     7681 taatgcattc tcgtgtttaa tcgacattaa tgtgggggga gaaagacatc ccatccctga
     7741 aaggtttttc cagggaatag tcaggacgca ttggctttca c
//
