LOCUS       V004769                 7304 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004769
VERSION     V004769
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Zero background yeast reporter plasmids
  JOURNAL   Gene 247 (1-2), 53-61 (2000)
   PUBMED   10773444
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-SEP-2003) Institute for Microbiology, Frankfurt
            University, Marie Curie Strasse 9, Frankfurt 60439, Germany
REFERENCE   3  (bases 1 to 7304)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7304)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 247
            (1-2), 53-61 (2000)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (11-SEP-2003) Institute for Microbiology, Frankfurt University,
            Marie Curie Strasse 9, Frankfurt 60439, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..658
                     /label="UAS deleted MEL1 promoter"
                     /note="UAS deleted MEL1 promoter"
                     /regulatory_class="promoter"
     CDS             659..2071
                     /gene="MEL1"
                     /label="Alpha-galactosidase 1"
                     /note="Alpha-galactosidase 1 from Saccharomyces cerevisiae.
                     Accession#: P04824"
     promoter        complement(2471..2489)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2510..2526)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2534..2550)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2558..2588)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2603..2624)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2912..3500)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3674..4531)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4532..4636)
                     /label="AmpR promoter"
     misc_feature    4673..5176
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        5435..5655
                     /label="URA3 promoter"
     CDS             5656..6456
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(6588..7043)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7188..7204
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7211..7229
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7262..7278
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 agctttcttt tttactttaa tattatgtat actgaaaaat gcacgtgatg ataagacttt
       61 ggaaatttgt gtaaaacccc catttttttt tgctgctgct attgccaaag ncaacaagtc
      121 ttcaggagac atcaacacta agtttctacc ccgtcttccc ctagaattct ttctgtacgc
      181 tcagggtggg cctttaaagg atagcaccct accgaagtcg acttctaagt aaacaccatt
      241 actaggagat gactaaatct ggaaaacaca tggtggtctg aatgcgtcta gtctctgcca
      301 taaacataac atgtttgttt taatgcattc tcgtgtttaa tcgacattaa tgtgccggga
      361 gaaagacatc ccatccctga aaggtttttc cagggaatag tcaggacgca ttggctttca
      421 ctcgagaaga agaaaggaag acatgtatta cattatccaa caaaaaatgg ttcttgacgt
      481 ctacaaatca agaatcttaa agacattgaa cgaagtagct gaataaaaat tatgaaaact
      541 ataaaaacta taaaaactgt acttaagtcc tcaataaaac ataaacttct tactgtataa
      601 ggttttcgat aatttcttac ttgattctag gagagcaacg gtaataaaag caacgacgat
      661 gtttgctttc tactttctca ccgcatgcat cagtttgaag ggcgtttttg gggtgtctcc
      721 gagttacaat ggccttggtc tcactccaca gatgggttgg gacaactgga atacgtttgc
      781 ctgcgatgtc agtgaacagc tacttctaga caccgctgat agaatttctg acttggggct
      841 aaaggatatg ggttacaagt atatcattct ggatgactgc tggtctagcg gcagagattc
      901 cgacggtttc ctcgttgcag atgaacaaaa atttcccaat ggtatgggcc atgttgcaga
      961 ccacctgcat aataacagct ttcttttcgg tatgtattcg tctgctggtg agtacacctg
     1021 tgctggatat cctgggtctc tgggtcgtga ggaagaagat gcacagttct ttgcaaataa
     1081 ccgcgttgac tacttgaagt acgataattg ttacaataag ggtcagtttg gtacaccgga
     1141 aatttcttac caccgttaca aggccatgtc agatgctttg aataaaactg gtaggcctgt
     1201 attctattct ctatgtaact ggggtcagga tttaacattt tactggggct ctggtatcgc
     1261 caattcttgg agaatgagtg gagatgttac tgctgagttc actcgtccag atagcagatg
     1321 tccctgtgat ggcgatgaat acgattgcaa gtacgccggt ttccattgtt ctattatgaa
     1381 tattcttaac aaggcagctc caatggggca aaatgcaggt gttggtggtt ggaatgatct
     1441 ggacaatcta gaggttggtg tcgggaattt gactgacgat gaggaaaagg cacatttctc
     1501 tatgtgggca atggtaaagt ctccacttat cattggtgcc aatgtgaata acttaaaggc
     1561 atcttcgtac tcaatctata gtcaagcctc tgtcatcgca attaatcaag attcaaatgg
     1621 tattccagca acaagagtct ggagatatta tgtttcagac acagatgaat atggacaagg
     1681 tgaaattcaa atgtggagtg gtcctcttga caatggtgat caagtggttg ctttattgaa
     1741 tggaggaagc gtatctagac caatgaacac gaccttggaa gagatttttt ttgacagcaa
     1801 tctgggttca aagaaactga catcgacttg ggatatctac gacctatggg ccaacagagt
     1861 tgacaactcg acagcgtctg ctatccttgg acggaataag acagccaccg gtattctcta
     1921 caatgctacg gagcaatcct acaaagacgg tttgtctaag aatgatacaa gactgtttgg
     1981 tcagaaaatt ggtagtcttt ctccaaatgc tatacttaac acgactgttc cagctcacgg
     2041 tatcgccttc tataggttga gaccctcttc ttgagcttat tgttgagcaa agcagggcga
     2101 gaagtattga tgattgttaa aaagttcatg aaaaaaatac tactcgaata tttattcaga
     2161 gtaactaaat aataaacgac agaatagcct atcaggtatt ccaatagttt tcgttttgta
     2221 ggtacacaat ctgaagccct tgaacttttt ctcgtttaca tacttcattg cattagcgat
     2281 atttcacatg tgctatacta gtgacttttg taaaatactt ttctgccaat gtgattctag
     2341 aattgtatga catttcacga agaggaacaa cagcttcagg agtacataca aacgacgaaa
     2401 tcatcatatc agcaaaaccg gatatcgaag aagcagaagg cgctagtcta caggtaccca
     2461 gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg tcatagctgt
     2521 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     2581 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     2641 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     2701 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     2761 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     2821 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     2881 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctcggcccc cctgacgagc
     2941 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     3001 aggcgttccc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     3061 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
     3121 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     3181 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     3241 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     3301 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     3361 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     3421 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     3481 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     3541 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     3601 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     3661 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     3721 gttcatccat agttgcctga ctgcccgtcg tgtagataac tacgatacgg gagggcttac
     3781 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     3841 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     3901 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     3961 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4021 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4081 gaaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4141 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4201 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4261 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4321 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4381 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     4441 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     4501 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     4561 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     4621 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgggtccttt tcatcacgtg
     4681 ctataaaaat aattataatt taaatttttt aatataaata tataaattaa aaatagaaag
     4741 taaaaaaaga aattaaagaa aaaatagttt ttgttttccg aagatgtaaa agactctagg
     4801 gggatcgcca acaaatacta ccttttatct tgctcttcct gctctcaggt attaatgccg
     4861 aattgtttca tcttgtctgt gtagaagacc acacacgaaa atcctgtgat tttacatttt
     4921 acttatcgtt aatcgaatgt atatctattt aatctgcttt tcttgtctaa taaatatata
     4981 tgtaaagtac gctttttgtt gaaatttttt aaacctttgt ttattttttt ttcttcattc
     5041 cgtaactctt ctaccttctt tatttacttt ctaaaatcca aatacaaaac ataaaaataa
     5101 ataaacacag agtaaattcc caaattattc catcattaaa agatacgagg cgcgtgtaag
     5161 ttacaggcaa gcgatccgtc ctaagaaacc attattatca tgacattaac ctataaaaat
     5221 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     5281 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     5341 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     5401 tcagagcaga ttgtactgag agtgcaccac gcttttcaat tcaattcatc attttttttt
     5461 tattcttttt tttgatttcg gtttctttga aatttttttg attcggtaat ctccgaacag
     5521 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
     5581 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
     5641 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
     5701 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
     5761 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
     5821 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
     5881 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
     5941 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
     6001 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
     6061 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
     6121 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
     6181 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
     6241 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa
     6301 cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga
     6361 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
     6421 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
     6481 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attaccctgc
     6541 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt
     6601 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     6661 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     6721 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     6781 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     6841 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6901 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6961 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     7021 taatgcgccg ctacagggcg cgtcgcgcca ttcgccattc aggctgcgca actgttggga
     7081 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaggggg gatgtgctgc
     7141 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     7201 cagtgaattg taatacgact cactataggg cgaattggag ctccaccgcg gtggcggccg
     7261 ctctagaact agtggatccc ccgggctgca ggaattcgat atca
//
