LOCUS       V004770                 7305 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004770
VERSION     V004770
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Zero background yeast reporter plasmids
  JOURNAL   Gene 247 (1-2), 53-61 (2000)
   PUBMED   10773444
REFERENCE   2  (bases 1 to 7305)
  AUTHORS   Melcher K, Sharma B, Ding WV, Nolden M.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-SEP-2003) Institute for Microbiology, Frankfurt
            University, Marie Curie Strasse 9, Frankfurt 60439, Germany
REFERENCE   3  (bases 1 to 7305)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7305)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene 247
            (1-2), 53-61 (2000)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (10-SEP-2003) Institute for Microbiology, Frankfurt University,
            Marie Curie Strasse 9, Frankfurt 60439, Germany"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..659
                     /label="UAS deleted MEL1 promoter"
                     /note="UAS deleted MEL1 promoter"
                     /regulatory_class="promoter"
     CDS             660..2072
                     /gene="MEL1"
                     /label="Alpha-galactosidase 1"
                     /note="Alpha-galactosidase 1 from Saccharomyces cerevisiae.
                     Accession#: P04824"
     promoter        complement(2472..2490)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2511..2527)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2535..2551)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2559..2589)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2604..2625)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2913..3501)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3675..4532)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4533..4637)
                     /label="AmpR promoter"
     misc_feature    4674..5177
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        5436..5656
                     /label="URA3 promoter"
     CDS             5657..6457
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(6589..7044)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7189..7205
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7212..7230
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7263..7279
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 agctttcttt tttactttaa tattatgtat actgaaaaat gcacgtgatg ataagacttt
       61 ggaaatttgt gtaaaacccc catttttttt tgctgctgct attgccaaag tcaacaagtc
      121 ttcaggagac atcaacacta agtttctacc ccgtcttccc ctagaattct ttctgtacgc
      181 tcagggtggg cctttaaagg atagcaccct accgaagtcg acttctaagt aaacaccatt
      241 actaggagat gactaaatct ggaaaaccac atggtggtct gaatgcgtct agtctctgcc
      301 ataaacataa catgtttgtt ttaatgcatt ctcgtgttta atcgacatta atgtgggggg
      361 agaaagacat cccatccctg aaaggttttt ccagggaata gtcaggacgc attggctttc
      421 actcgagaag aagaaaggaa gacatgtatt acattatcca acaaaaaatg gttcttgacg
      481 tctacaaatc aagaatctta aagacattga acgaagtagc tgaataaaaa ttatgaaaac
      541 tataaaaact ataaaaactg tacttaagtc ctcaataaaa cataaacttc ttactgtata
      601 aggttttcga taatttctta cttgattcta ggagagcaac ggtaataaaa gcaacgacga
      661 tgtttgcttt ctactttctc accgcatgca tcagtttgaa gggcgttttt ggggtgtctc
      721 cgagttacaa tggccttggt ctcactccac agatgggttg ggacaactgg aatacgtttg
      781 cctgcgatgt cagtgaacag ctacttctag acaccgctga tagaatttct gacttggggc
      841 taaaggatat gggttacaag tatatcattc tggatgactg ctggtctagc ggcagagatt
      901 ccgacggttt cctcgttgca gatgaacaaa aatttcccaa tggtatgggc catgttgcag
      961 accacctgca taataacagc tttcttttcg gtatgtattc gtctgctggt gagtacacct
     1021 gtgctggata tcctgggtct ctgggtcgtg aggaagaaga tgcacagttc tttgcaaata
     1081 accgcgttga ctacttgaag tacgataatt gttacaataa gggtcagttt ggtacaccgg
     1141 aaatttctta ccaccgttac aaggccatgt cagatgcttt gaataaaact ggtaggcctg
     1201 tattctattc tctatgtaac tggggtcagg atttaacatt ttactggggc tctggtatcg
     1261 ccaattcttg gagaatgagt ggagatgtta ctgctgagtt cactcgtcca gatagcagat
     1321 gtccctgtga tggcgatgaa tacgattgca agtacgccgg tttccattgt tctattatga
     1381 atattcttaa caaggcagct ccaatggggc aaaatgcagg tgttggtggt tggaatgatc
     1441 tggacaatct agaggttggt gtcgggaatt tgactgacga tgaggaaaag gcacatttct
     1501 ctatgtgggc aatggtaaag tctccactta tcattggtgc caatgtgaat aacttaaagg
     1561 catcttcgta ctcaatctat agtcaagcct ctgtcatcgc aattaatcaa gattcaaatg
     1621 gtattccagc aacaagagtc tggagatatt atgtttcaga cacagatgaa tatggacaag
     1681 gtgaaattca aatgtggagt ggtcctcttg acaatggtga tcaagtggtt gctttattga
     1741 atggaggaag cgtatctaga ccaatgaaca cgaccttgga agagattttt tttgacagca
     1801 atctgggttc aaagaaactg acatcgactt gggatatcta cgacctatgg gccaacagag
     1861 ttgacaactc gacagcgtct gctatccttg gacggaataa gacagccacc ggtattctct
     1921 acaatgctac ggagcaatcc tacaaagacg gtttgtctaa gaatgataca agactgtttg
     1981 gtcagaaaat tggtagtctt tctccaaatg ctatacttaa cacgactgtt ccagctcacg
     2041 gtatcgcctt ctataggttg agaccctctt cttgagctta ttgttgagca aagcagggcg
     2101 agaagtattg atgattgtta aaaagttcat gaaaaaaata ctactcgaat atttattcag
     2161 agtaactaaa taataaacga cagaatagcc tatcaggtat tccaatagtt ttcgttttgt
     2221 aggtacacaa tctgaagccc ttgaactttt tctcgtttac atacttcatt gcattagcga
     2281 tatttcacat gtgctatact agtgactttt gtaaaatact tttctgccaa tgtgattcta
     2341 gaattgtatg acatttcacg aagaggaaca acagcttcag gagtacatac aaacgacgaa
     2401 atcatcatat cagcaaaacc ggatatcgaa gaagcagaag gcgctagtct acaggtaccc
     2461 agcttttgtt ccctttagtg agggttaatt ccgagcttgg cgtaatcatg gtcatagctg
     2521 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     2581 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     2641 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     2701 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     2761 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     2821 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     2881 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctcggccc ccctgacgag
     2941 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3001 caggcgttcc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3061 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     3121 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3181 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3241 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3301 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     3361 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3421 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3481 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3541 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     3601 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     3661 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     3721 cgttcatcca tagttgcctg actgcccgtc gtgtagataa ctacgatacg ggagggctta
     3781 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     3841 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     3901 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     3961 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4021 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4081 tgaaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4141 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4201 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4261 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4321 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4381 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4441 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4501 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4561 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     4621 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgggtcctt ttcatcacgt
     4681 gctataaaaa taattataat ttaaattttt taatataaat atataaatta aaaatagaaa
     4741 gtaaaaaaag aaattaaaga aaaaatagtt tttgttttcc gaagatgtaa aagactctag
     4801 ggggatcgcc aacaaatact accttttatc ttgctcttcc tgctctcagg tattaatgcc
     4861 gaattgtttc atcttgtctg tgtagaagac cacacacgaa aatcctgtga ttttacattt
     4921 tacttatcgt taatcgaatg tatatctatt taatctgctt ttcttgtcta ataaatatat
     4981 atgtaaagta cgctttttgt tgaaattttt taaacctttg tttatttttt tttcttcatt
     5041 ccgtaactct tctaccttct ttatttactt tctaaaatcc aaatacaaaa cataaaaata
     5101 aataaacaca gagtaaattc ccaaattatt ccatcattaa aagatacgag gcgcgtgtaa
     5161 gttacaggca agcgatccgt cctaagaaac cattattatc atgacattaa cctataaaaa
     5221 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     5281 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     5341 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     5401 atcagagcag attgtactga gagtgcacca cgcttttcaa ttcaattcat catttttttt
     5461 ttattctttt ttttgatttc ggtttctttg aaattttttt gattcggtaa tctccgaaca
     5521 gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca tatgtagtgt
     5581 tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa aaacctgcag
     5641 gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta ctcatcctag
     5701 tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact tgtgtgcttc
     5761 attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag gtcccaaaat
     5821 ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg gcacagttaa
     5881 gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca gaaaatttgc
     5941 tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa tagcagaatg
     6001 ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg gtttgaagca
     6061 ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag aattgtcatg
     6121 caagggctcc ctatctactg gagaatatac taagggtact gttgacattg cgaagagcga
     6181 caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag atgaaggtta
     6241 cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg cattgggtca
     6301 acagtataga accgtggatg atgtggtctc tacaggatct gacattatta ttgttggaag
     6361 aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca gaaaagcagg
     6421 ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat tataagtaaa
     6481 tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt tattaccctg
     6541 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg
     6601 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     6661 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     6721 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     6781 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     6841 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     6901 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     6961 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     7021 ttaatgcgcc gctacagggc gcgtcgcgcc attcgccatt caggctgcgc aactgttggg
     7081 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaagggg ggatgtgctg
     7141 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     7201 ccagtgaatt gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc
     7261 gctctagaac tagtggatcc cccgggctgc aggaattcga tatca
//
