LOCUS       Exported               10504 bp DNA     circular SYN 05-SEP-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(46..1242)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             1348..1995
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             complement(2822..3904)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3905..3982)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        4216..4244
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4252..4268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4356..5072
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     rep_origin      complement(5681..6226)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     rep_origin      complement(6935..7129)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7198..8268)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8700..9326)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tagttctagc tagttctaga gcggccccta tcgtttccac gatcagcgat cggctcgttg
       61 ccctgcgccg ctccaaagcc cgcgacgcag cgccggcagg cagagcaagt agagggcagc
      121 gcctgcaatc catgcccacc cgttccacgt tgttatagaa gccgcataga tcgccgtgaa
      181 gaggaggggt ccgacgatcg aggtcaggct ggtgagcgcc gccagtgagc cttgcagctg
      241 cccctgacgt tcctcatcca cctgcctgga caacattgct tgcagcgccg gcattccgat
      301 gccacccgaa gcaagcagga ccatgatcgg gaacgccatc catccccgtg tcgcgaaggc
      361 aagcaggatg tagcctgtgc cgtcggcaat cattccgagc atgagtgccc gcctttcgcc
      421 gagccgggcg gctacagggc cggtgatcat tgcctgggcg agtgaatgca gaatgccaaa
      481 tgcggcaagc gaaatgccga tcgtggtcgc gtcccagtga aagcgatcct cgccgaaaat
      541 gacccaaagc gcggccggca cctgtccgac aagttgcatg atgaagaaga ccgccatcag
      601 ggcggcgacg acggtcatgc cccgggccca ccggaacgaa gcgagcgggt tgagagcctc
      661 ccggcgtaac ggccggcgtt cgcctttgtg cgactccggc aaaaggaaac agcccgtcag
      721 gaaattgagg ccgttcaagg ctgccgcggc gaagaacgga gcgtgggggg agaaaccgcc
      781 catcagccca ccgagcacag gtcccgcgac catcccgaac ccgaaacagg cgctcatgaa
      841 gccgaagtgc cgcgcgcgct catcgccatc agtgatatcg gcaatataag cgccggctac
      901 cgccccagtc gccccggtga tgccggccac gatccgcccg atatagagaa cccaaaggaa
      961 aggcgctgtc gccatgatgg cgtagtcgac agtggcgccg gccagcgaga cgagcaagat
     1021 tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg caaattgcac
     1081 caacgcatac agcgccagca gaatgccata gtgggcggtg acgtcgttcg agtgaaccag
     1141 atcgcgcagg aggcccggca gcaccggcat aatcaggccg atgccgacag cgtcgagcgc
     1201 gacagtgctc agaattacga tcaggggtat gttgggtttc acgtctggcc tccggaccag
     1261 cctccgctgg tccgattgaa cgcgcggatt ctttatcact gataagttgg tggacatatt
     1321 atgtttatca gtgataaagt gtcaagcatg acaaagttgc agccgaatac agtgatccgt
     1381 gccgccctgg acctgttgaa cgaggtcggc gtagacggtc tgacgacacg caaactggcg
     1441 gaacggttgg gggttcagca gccggcgctt tactggcact tcaggaacaa gcgggcgctg
     1501 ctcgacgcac tggccgaagc catgctggcg gagaatcata cgcattcggt gccgagagcc
     1561 gacgacgact ggcgctcatt tctgatcggg aatgcccgca gcttcaggca ggcgctgctc
     1621 gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc gcagatggaa
     1681 acggccgacg cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg ggacgccgtc
     1741 aatgcgctga tgacaatcag ctacttcact gttggggccg tgcttgagga gcaggccggc
     1801 gacagcgatg ccggcgagcg cggcggcacc gttgaacagg ctccgctctc gccgctgttg
     1861 cgggccgcga tagacgcctt cgacgaagcc ggtccggacg cagcgttcga gcagggactc
     1921 gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg tcaggaacgt tgaaggaccg
     1981 agaaagggtg acgattgatc aggaccgctg ccggagcgca acccactcac tacagcagag
     2041 ccatgtagac aacatcccct ccccctttcc accgcgtcag acgcccgtag cagcccgcta
     2101 cgggcttttt catgccctgc cctagcgtcc aagcctcacg gccgcgctcg gcctctctgg
     2161 cggccttctg gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct
     2221 gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt
     2281 aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg
     2341 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccgcc
     2401 gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta
     2461 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc
     2521 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag
     2581 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat
     2641 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga
     2701 atccggtgag aatggcaaaa gctggccgcc acggcgatat cggatccgaa cgccagcaag
     2761 acgtagccca gcgcgtcggc cagcttgcaa ttcgcgctaa cttacattaa ttgcgttgcg
     2821 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2881 acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg
     2941 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
     3001 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
     3061 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
     3121 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
     3181 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
     3241 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
     3301 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
     3361 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
     3421 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
     3481 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
     3541 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
     3601 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
     3661 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
     3721 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca
     3781 tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc
     3841 gggaaacggt ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt
     3901 tcacattcac caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg
     3961 ttttgcacca ttcgatggtg tcaacgtaaa tgcatgccgc ttcgccttcg cgcgcgaatt
     4021 gcaagctgat ccgggcttat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc
     4081 atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa
     4141 ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata
     4201 ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg
     4261 gataacaatt tcacacagga aacagaattc gagctcccgg gtaccggaag cttcgtactc
     4321 gaggttcact agtgtctgca gcgagagatc tgcccatggt gagcaagggc gaggagctgt
     4381 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4441 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     4501 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     4561 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     4621 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     4681 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     4741 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     4801 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     4861 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     4921 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     4981 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5041 ggatcactct cggcatggac gagctgtaca agtagtcgag gcctcggact agcggatcag
     5101 tgagggtttg caactgcggg tcaaggatct ggatttcgat cacggcacga tcatcgtgcg
     5161 ggagggcaag ggctccaagg atcgggcctt gatgttaccc gagagcttgg cacccagcct
     5221 gcgcgagcag gggaattgat ccggtggatg accttttgaa tgacctttaa tagattatat
     5281 tactaattaa ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcacaaaa
     5341 cggtttacaa gcataaagct cctcggacta cctggtatga gtcagcaaca ccttcttcac
     5401 gaggcagacc tcagcgctag cggagtgtat actggcttac tatgttggca ctgatgaggg
     5461 tgtcagtgaa gtgcttcatg tggcaggaga aaaaaggctg caccggtgcg tcagcagaat
     5521 atgtgataca ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga
     5581 ctgcggcgag cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag
     5641 atacttaaca gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc
     5701 ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat
     5761 aaagatacca ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc
     5821 ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc
     5881 agttccgggt aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc
     5941 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa
     6001 gcaccactgg cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc
     6061 cggttaaggc taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc
     6121 tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt
     6181 ttcgttttca gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat
     6241 taaggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     6301 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     6361 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     6421 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     6481 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     6541 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     6601 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     6661 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgcctggt atgagtcagc
     6721 aacaccttct tcacgaggca gacctcagcg ccggcggtcg agtggcgacg gcgcggcttg
     6781 tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc cagcggccgc
     6841 gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt
     6901 ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc gggttttaag
     6961 agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct cttttatatc
     7021 agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa tgtacgggtt
     7081 ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag gaaagagacc
     7141 ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg tacattagga
     7201 accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga tcgggccagc
     7261 ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga tcagcttgcg
     7321 cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt
     7381 gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg
     7441 gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt
     7501 gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt actccggccg
     7561 cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct cggataccgt
     7621 caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa
     7681 ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa
     7741 cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc ccttcccttc
     7801 ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc
     7861 ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt ctggaagctc
     7921 gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc
     7981 catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga aaaaatctgg
     8041 ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg gcggttgccg
     8101 ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc gtgcttctgc
     8161 ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca ggtcatcacc
     8221 cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc cgattcctcg
     8281 ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct tacgcctggc
     8341 caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg ttgttcttga
     8401 ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc atttgctcat
     8461 ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct tgccttggcg
     8521 taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt tgacccgctt
     8581 catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg
     8641 acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc attaactcaa
     8701 atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc gtcgccctcg
     8761 ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc
     8821 gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc aacctcaccg
     8881 ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc
     8941 gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca tatgtcgtaa
     9001 gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga cacagccaag
     9061 tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat ggccttcacg
     9121 tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc ttcccgcacg
     9181 gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac atcggccccg
     9241 gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga cccgcctttc
     9301 tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta tttactcatc
     9361 gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca catcaccttt
     9421 ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc gccagcttgg
     9481 catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc gcgggcggct
     9541 cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg aaaaacggtt
     9601 cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat tctcggcggc
     9661 cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc
     9721 ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc
     9781 aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg
     9841 cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc gggccttggc
     9901 ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact cggcaatgcc
     9961 ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc acggctctgc
    10021 caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg
    10081 ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg
    10141 gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa
    10201 cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc
    10261 ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg
    10321 tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga caagaaaacg
    10381 ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt
    10441 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
    10501 gatc
//
