LOCUS       40924_30485        5632 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-lama2-Glob, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-SEP-2011) Australian Regenerative Medicine Institute, 
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   2  (bases 1 to 5632)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-SEP-2011) Australian Regenerative Medicine Institute, Monash 
            University, Building 75, Melbourne, VIC 3800, Australia"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      669..678
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    675..3757
                     /note="laminin alpha 2 chain fragment C-terminal globular
                     domain fused to the N-terminal secretion signal"
     protein_bind    complement(3757..3856)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(3874..3892)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3897..3913)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4026..4832
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      4982..5570
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc caccatggac acgcggagtt tagtggcgct gctcgggctg ctgtggcggt
      721 gttgtggagc tccgcagagg ggtcttttct tgtccggtgg agactgcatc cgcacctacc
      781 ggccagagat caagaagggc cgctacaaca ccatcatcct ccacctcaag accctcgctg
      841 ccgataacct gctcttctac ctgggatccg ccaaatacgt ggatttcttg gccatcgaga
      901 tgcgtaaggg gaaggtgaac ttcctgtggg atgtggggtc aggggtcggc cgagtggaat
      961 atccagatct gatcatcaac gatggaaact ggcaccgaat cgaagcctca cgtattggtc
     1021 tgaacggcag tatctcagtc cgtgcgctcg agggacccaa aacaggcatc ttaccttcac
     1081 tcagaagtgc aaaagcaccg gagacctaca ctgtgctgga cgtggaccag aacgcttacc
     1141 tgtttgtcgg aggcatgctg ggatcagtga agaaagcaga tgctgtgaaa accaccacat
     1201 tctcagggtg tatgggtgag acgtctctgg atggaaagcc catcggcctc tggaactaca
     1261 gagagagaga cggggactgt gctggatgtg ttgtcagtcc tcagcctgca gacactgaag
     1321 gaacggttca gtttgatgga gatggttatg cgtccgtcag tcgccccacc aggtggaacc
     1381 caaatgtctc cactgtcatg ttcaagtttc gcaccttctc cacacactcc gtcatcatgt
     1441 acttcgccac caaagacatg aaagacttca tgagtgctga actgagtgat ggcagggtga
     1501 aagtgagtta tgatctgggc tctggcaccg gctccatcat cagtgataaa cgctataatg
     1561 acggcaagtg gaagtccttc accatgtccc gcatgaagaa agaagccacc atcaccatcg
     1621 tcaacatgga cactaatgag aatgagcggc tgctgatggc gtcaccaggg ggcgctacag
     1681 gccttaacct gcgagacaac gagcgcatat ttttcggagg attgcccaaa gctggaaaat
     1741 acaggtcaga ggtggttttg aagaggtttt ctggctgtat gaaggatatc gaggtctccc
     1801 gaacgccata taatctgctg agcagtccag attacacagg actgaccaaa ggctgctcca
     1861 ttgagaatct gcacacagtg agtttcccgc gggccgggtt catggagctg aatccggtgt
     1921 catttgccgt gggctcggag atctcgctct ccttcagcac caggagtgaa gacgggctga
     1981 tactgttcgg cagaggagga ctgacctcca tgacggagct gacacaggga ctcacgccag
     2041 tgcacatccc acgccgctca cgcagacaga ccggagagcc cttcctctct gtgcagctca
     2101 gtaaaggggc actggaggtg ctggtgttca ccggctccag aaaccctcgc agagttttcc
     2161 gaaaaccaga tcagggaatc ctgcatgatg gccgagagca ctcactgcgc atccagaggc
     2221 tcccgggagc atccttcact gtgcaggtgg acgaggagcc gctggtgcag cagcccttac
     2281 ccaacgacct cccactcaac atccaccgcc tcttcatcgg aggaatcccg cctgatgtac
     2341 aaaccggcgt ccacaggccc agcgtgccgt tcgagggctg catctggaac ctcctgatca
     2401 acactgtccc ggtggacttc tcccagcctg tggcctttga gaatgcagag attggccact
     2461 gccctgacct ggccccggcc ccagctccgc ctcttgagga ggtggaaaag gaagaggctc
     2521 cgcctacacc agcagaagca gttcgtcctc caccagctcc agcctccact gctacacccc
     2581 ttgccgaagc caagcccatc acagacacac ccaccagtgt caaagagccc gcggtgtcgt
     2641 gcgcagcaga ggcggagcct gcagtgctgg ggctggccaa acagttcggt ctgtccagaa
     2701 acagtcacat gagctttgag tttgatgaca gaaaggtgat gaacaggttg gtcattgagt
     2761 tcgagatgcg cacagagaca gactctggtc tggttttcta catggccaga ataaaccacg
     2821 cagacttcgc caccatccag ctgaaggagg gaatggctca cctaagcttc gacctgggca
     2881 gcgggaacac ctccgtcagc gtgccgcgca tcatcaacga cgggcagtgg cacaagatcc
     2941 gtgtgatgag agacaaacag agaggagtcc tcacgataga cggccgctac agcaaacaca
     3001 ccaccagccc gaagaaagca gaaatcctgg atgtggtggg aatgctgtat gttggtggtt
     3061 taccgctcaa ctacaccacc aaacgcatcg gccctgtgct gtacagtatt gacgcttgca
     3121 ttaggaactt caagatgatg aatctgcctt tggatatgga gaatcccacc tccagctacc
     3181 gcgtgggctc ctgctttgcc aacccagaaa agggaaacta tttcaatggc acaggctatg
     3241 ccaaagctgt ggaggcatat cgagtgggta atgacgtgtc agtggatctg gagttcagga
     3301 cggctcagag ctctgctgtc ctggtgggcg tcagcagcca aaagatggac gggctgggca
     3361 tcgaactcgt aaatggaaag ctgttcttcc atgctgataa cggagtgggc cgcttcacag
     3421 ccgtgcatga gccgaggcag gaggcggggc tttgtgatgg gcagtggcac acagtctccg
     3481 cccacaagct gaagcaccgc ctggagctga cggtggacgg acagaagtct gaggcggcga
     3541 gtccagacat ccgctcgccc tccgcagaca ccaatgaccc actgtatgtg ggcggatacc
     3601 ccgagggtct gaagcagttc gggctcacca taaacacccc gtttaaaggc tgcatgagga
     3661 acgtgaagat cagtaaagct ggaaaaactc tgcaggtcca gatgaacaaa ctgctggagc
     3721 tcagaggagt tcagccgctc agctgccctg ctgcttaccc agctttcttg tacaaagttg
     3781 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     3841 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     3901 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     3961 ataaaataat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     4021 gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg
     4081 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     4141 atcgcttgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     4201 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     4261 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     4321 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     4381 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     4441 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     4501 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     4561 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     4621 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     4681 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     4741 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     4801 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
     4861 actggcagag cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta
     4921 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4981 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5041 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5101 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5161 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5221 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5281 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5341 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5401 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5461 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5521 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5581 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg tt
//
