LOCUS       40924_30475        3609 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-iCre, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Sztal TE, Zhao M, Williams C, Oorschot V, Parslow AC, Giousoh A, 
            Yuen M, Hall TE, Costin A, Ramm G, Bird PI, Busch-Nentwich EM, 
            Stemple DL, Currie PD, Cooper ST, Laing NG, Nowak KJ, 
            Bryson-Richardson RJ.
  TITLE     Zebrafish models for nemaline myopathy reveal a spectrum of nemaline
            bodies contributing to reduced muscle function
  JOURNAL   Acta Neuropathol. 130 (3), 389-406 (2015)
  PUBMED    25931053
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Cre-lox in zebrafish
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 3609)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2013) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   4  (bases 1 to 3609)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3609)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Neuropathol."; date: "2015"; volume: "130"; issue: "3"; pages: 
            "389-406"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (21-OCT-2013) Institute for Molecular Bioscience, University of 
            Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3609
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      671..680
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             683..703
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             701..1729
                     /label=Cre
                     /note="site-specific recombinase"
     protein_bind    complement(1734..1833)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1851..1869)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1874..1890)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2003..2809
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2959..3547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gccaccatgg tgcccaagaa gaagaggaaa gtctccaacc tgctgactgt
      721 gcaccaaaac ctgcctgccc tccctgtgga tgccacctct gatgaagtca ggaagaacct
      781 gatggacatg ttcagggaca ggcaggcctt ctctgaacac acctggaaga tgctcctgtc
      841 tgtgtgcaga tcctgggctg cctggtgcaa gctgaacaac aggaaatggt tccctgctga
      901 acctgaggat gtgagggact acctcctgta cctgcaagcc agaggcctgg ctgtgaagac
      961 catccaacag cacctgggcc agctcaacat gctgcacagg agatctggcc tgcctcgccc
     1021 ttctgactcc aatgctgtgt ccctggtgat gaggagaatc agaaaggaga atgtggatgc
     1081 tggggagaga gccaagcagg ccctggcctt tgaacgcact gactttgacc aagtcagatc
     1141 cctgatggag aactctgaca gatgccagga catcaggaac ctggccttcc tgggcattgc
     1201 ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc agagtgaagg acatctcccg
     1261 caccgatggt gggagaatgc tgatccacat tggcaggacc aagaccctgg tgtccacagc
     1321 tggtgtggag aaggccctgt ccctgggggt taccaagctg gtggagagat ggatctctgt
     1381 gtctggtgtg gctgatgacc ccaacaacta cctgttctgc cgggtcagaa agaatggtgt
     1441 ggctgcccct tctgccacct cccaactgtc cacccgggcc ctggaaggga tctttgaggc
     1501 cacccaccgc ctgatctatg gtgccaagga tgactctggg cagagatacc tggcctggtc
     1561 tggccactct gccagagtgg gtgctgccag ggacatggcc agggctggtg tgtccatccc
     1621 tgaaatcatg caggctggtg gctggaccaa tgtgaacatt gtgatgaact acatcagaaa
     1681 cctggactct gagactgggg ccatggtgag gctgctcgag gatggggact gacacccagc
     1741 tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt gcaacgaaca
     1801 ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc cctatagtga
     1861 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     1921 atgttacatt gcacaagata aaataatatc atcatgaaca ataaaactgt ctgcttacat
     1981 aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga ggccgcgatt
     2041 aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     2101 atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa
     2161 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     2221 gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     2281 gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag aatatcctga
     2341 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     2401 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     2461 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     2521 tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt cagtcgtcac
     2581 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     2641 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     2701 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     2761 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt
     2821 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc
     2881 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     2941 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3061 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3601 tcacatgtt
//
