LOCUS       40924_30480        3907 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-ILK-NS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Sztal TE, Sonntag C, Hall TE, Currie PD.
  TITLE     Epistatic dissection of laminin-receptor interactions in dystrophic 
            zebrafish muscle
  JOURNAL   Hum. Mol. Genet. 21 (21), 4718-4731 (2012)
  PUBMED    22859503
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Hall TE, Sztal TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUN-2012) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 3907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum. Mol. 
            Genet."; date: "2012"; volume: "21"; issue: "21"; pages: "4718-4731"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUN-2012) Institute for Molecular Bioscience, University of 
            Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      669..678
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    675..2033
                     /label=similar to zebrafish integrin linked kinase (ilk)
                     /note="similar to zebrafish integrin linked kinase (ilk)"
     protein_bind    complement(2032..2131)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2149..2167)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2172..2188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2301..3107
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3257..3845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgc caccatggat gacatcttca ctcagtgccg tgaaggcaac gcggtagccg
      721 ttcgcctctg gctggacaac acagagaatg acctaaacca gggggatgat cacggcttca
      781 gtccgttgca ctgggcgtgc cgcgagggcc gttcgggtgt tgtggatatg ctcatcatga
      841 gaggtgctcg tatcaatgtg atgaaccgcg gcgacgacac acccctgcac ctggcagcga
      901 gccatggcca tcgtgacatt ctggccaagc tgattcagtg taaagcagat accaatgcgg
      961 caaacgagca cggaaacacc cctctacatt atgcctgctt ctgggcccac gatcaggtgg
     1021 cggaggatct ggtcagtaat ggagctcagg tgagcatctg caacaagtat ggagaaactc
     1081 caatggacaa ggccaaacca ccgcttgcag caaatctaaa agacctggca gagaaacaag
     1141 gacagagcct gtcaaaagtc ccttacaagg acaatttctg gaaaggaaca actcgaacac
     1201 gaccacgtaa tggcacacta aacaagcaag ctggcataga ttataagcag cttaacatgc
     1261 tgaacaagat caacgagaac cactccggcg agctgtggaa gggccgctgg cagggcacag
     1321 agattgtggt caagatgctg catgtgcggg aatggaccac cagaaagagc cgagacttta
     1381 atgaggagta ccctaaacta agaatcttct ctcacccaaa cgtgttgccc gtgttggggg
     1441 cgtgtcagtc tccacctgcc cctcacccaa tcatcatcac acactctatg ccctacggtt
     1501 ccctctacaa cttgctgcat gagggcacca acttagtagt ggatcaaaac caggctgtga
     1561 aatttgctct ggacattgcc tgtggaatgg ctttcctaca cactcttgag cccatgatcc
     1621 cccgccatta tctcaacagc aagagtgtca tgattgacga ggacatgacg gccaggataa
     1681 gcatggcaga tgtaaagttg tcatttcagt gtcctggcag gatgtactct cctgcatggg
     1741 tggcacctga agctctgcag aagaagccag aggagatcaa ccggcgctca gctgacatgt
     1801 ggagctacgc cgtgctgctc tgggagctgg tgagcagaga agtgccgttt gctgacctct
     1861 caaacatgga gatcggcatg aaggtggcgc tagagggact acggcccacc atcccacccg
     1921 gcatctcccc tcacatctgc aaactcatga agatctgcat gaacgaagac cctgcaaaga
     1981 ggcccaaatt cgacatgatc gtgcccatcc tggagaagat gcaagacaaa tacccagctt
     2041 tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg
     2101 tcactatcag tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt
     2161 cgtattacat ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat
     2221 gttacattgc acaagataaa ataatatcat catgaacaat aaaactgtct gcttacataa
     2281 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg ccgcgattaa
     2341 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat
     2401 caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg tttctgaaac
     2461 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga
     2521 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt
     2581 tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa tatcctgatt
     2641 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg
     2701 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa
     2761 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg
     2821 aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca gtcgtcactc
     2881 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg
     2941 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc
     3001 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc
     3061 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg
     3121 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc gcaagctcat
     3181 gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac cccgtagaaa
     3241 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3301 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3361 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     3421 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3481 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3541 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3601 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3661 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3721 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3781 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3841 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3901 acatgtt
//
