LOCUS       40924_30465        3978 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-EGFP-Bcl2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2011) Australian Regenerative Medicine Institute, 
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   2  (bases 1 to 3978)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3978)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2011) Australian Regenerative Medicine Institute, Monash 
            University, Building 75, Melbourne, VIC 3800, Australia"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             674..1390
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(2103..2202)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2220..2238)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2243..2259)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2372..3178
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3328..3916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgg accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      721 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
      781 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      841 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      901 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
      961 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1021 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1081 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1141 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1201 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1261 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     1321 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1381 gctgtacaag tccggactca gatctcgact ggctaacgaa attagctatg acaatcggaa
     1441 tattgtggag aaatacctca agcataaact ttcaaagcga ggatatgtgt ggaaatgtca
     1501 gtcctctgct gaggaagatg acaccttcaa taaagcagtg gaggaatcct ctccaaactc
     1561 tgacaggagg cttcaggctc cctcagccgg cggagggaac aactctgaat gcctgatagc
     1621 ccgggtcact cgttcagacc ctcatttgag gctctaccgg gtgttacggg atgctggaga
     1681 tgaaatagaa aggatttacc aacgcgaatt tgaggaaatg tcccaacaaa tggtgttcaa
     1741 cccaaattct gcgcaacgca gctttctaac cgtggccgaa gagctcttta gagacggagt
     1801 gaactggggg cggatcattg cattcttcga gtttggtggg accatgtgcg tggaaagcgt
     1861 caaccgggag atggcgtccc aggtagataa tattgcacac tggatgactg actacctgaa
     1921 cgggccactg gaaaactgga tcgaggaaaa tggaggttgg gatgccttcg tggagatgta
     1981 cggtcagcag agagactctg tgttccaccc gttttcatac ctaacaaaag tgctcggctt
     2041 ggcggcgctg ggcttggcag gagtgaccat cggagccttt tttgctcaga agtgacagcc
     2101 tcacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2161 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2221 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2281 aaatctctga tgttacattg cacaagataa aataatatca tcatgaacaa taaaactgtc
     2341 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2401 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2461 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2521 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2581 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2641 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2701 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2761 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2821 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2881 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2941 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     3001 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     3061 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3121 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3181 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3241 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3301 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3361 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3421 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3481 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3541 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3601 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3661 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3721 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3781 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3841 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3901 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     3961 gccttttgct cacatgtt
//
