LOCUS       62056_16700        8824 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8824)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8824
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          739..1214
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1298..2797
                     /codon_start=1
                     /label=HIV-1 gag
                     /note="gag protein from human immunodeficiency virus 1"
                     /translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
                     VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
                     EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
                     FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
                     IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
                     ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
                     ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
                     HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
                     PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
     CDS             2593..5601
                     /codon_start=1
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    5629..5862
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_signal    6093..6487
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(6997..7585)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7759..8550)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(8551..8655)
                     /label=AmpR promoter
ORIGIN
        1 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
       61 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      121 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      181 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      241 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      301 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      361 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      421 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      481 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      541 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      601 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      661 atagaagaca ccgggaccga tccagcctcc cctcgaagct tacatgtggt accgagctcg
      721 gatcctgaga acttcagggt gagtctatgg gacccttgat gttttctttc cccttctttt
      781 ctatggttaa gttcatgtca taggaagggg agaagtaaca gggtacacat attgaccaaa
      841 tcagggtaat tttgcatttg taattttaaa aaatgctttc ttcttttaat atactttttt
      901 gtttatctta tttctaatac tttccctaat ctctttcttt cagggcaata atgatacaat
      961 gtatcatgcc tctttgcacc attctaaaga ataacagtga taatttctgg gttaaggcaa
     1021 tagcaatatt tctgcatata aatatttctg catataaatt gtaactgatg taagaggttt
     1081 catattgcta atagcagcta caatccagct accattctgc ttttatttta tggttgggat
     1141 aaggctggat tattctgagt ccaagctagg cccttttgct aatcatgttc atacctctta
     1201 tcttcctccc acagctcctg ggcaacgtgc tggtctgtgt gctggcccat cactttggca
     1261 aagcacgtga gatctgaatt cgagatctgc cgccgccatg ggtgcgagag cgtcagtatt
     1321 aagcggggga gaattagatc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     1381 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     1441 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     1501 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     1561 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     1621 aaacaaaagt aagaaaaaag cacagcaagc agcagctgac acaggacaca gcaatcaggt
     1681 cagccaaaat taccctatag tgcagaacat ccaggggcaa atggtacatc aggccatatc
     1741 acctagaact ttaaatgcat gggtaaaagt agtagaagag aaggctttca gcccagaagt
     1801 gatacccatg ttttcagcat tatcagaagg agccacccca caagatttaa acaccatgct
     1861 aaacacagtg gggggacatc aagcagccat gcaaatgtta aaagagacca tcaatgagga
     1921 agctgcagaa tgggatagag tgcatccagt gcatgcaggg cctattgcac caggccagat
     1981 gagagaacca aggggaagtg acatagcagg aactactagt acccttcagg aacaaatagg
     2041 atggatgaca cataatccac ctatcccagt aggagaaatc tataaaagat ggataatcct
     2101 gggattaaat aaaatagtaa gaatgtatag ccctaccagc attctggaca taagacaagg
     2161 accaaaggaa ccctttagag actatgtaga ccgattctat aaaactctaa gagccgagca
     2221 agcttcacaa gaggtaaaaa attggatgac agaaaccttg ttggtccaaa atgcgaaccc
     2281 agattgtaag actattttaa aagcattggg accaggagcg acactagaag aaatgatgac
     2341 agcatgtcag ggagtggggg gacccggcca taaagcaaga gttttggctg aagcaatgag
     2401 ccaagtaaca aatccagcta ccataatgat acagaaaggc aattttagga accaaagaaa
     2461 gactgttaag tgtttcaatt gtggcaaaga agggcacata gccaaaaatt gcagggcccc
     2521 taggaaaaag ggctgttgga aatgtggaaa ggaaggacac caaatgaaag attgtactga
     2581 gagacaggct aattttttag ggaagatctg gccttcccac aagggaaggc cagggaattt
     2641 tcttcagagc agaccagagc caacagcccc accagaagag agcttcaggt ttggggaaga
     2701 gacaacaact ccctctcaga agcaggagcc gatagacaag gaactgtatc ctttagcttc
     2761 cctcagatca ctctttggca gcgacccctc gtcacaataa agataggggg gcaattaaag
     2821 gaagctctat tagatacagg agcagatgat acagtattag aagaaatgaa tttgccagga
     2881 agatggaaac caaaaatgat agggggaatt ggaggtttta tcaaagtaag acagtatgat
     2941 cagatactca tagaaatctg cggacataaa gctataggta cagtattagt aggacctaca
     3001 cctgtcaaca taattggaag aaatctgttg actcagattg gctgcacttt aaattttccc
     3061 attagtccta ttgagactgt accagtaaaa ttaaagccag gaatggatgg cccaaaagtt
     3121 aaacaatggc cattgacaga agaaaaaata aaagcattag tagaaatttg tacagaaatg
     3181 gaaaaggaag gaaaaatttc aaaaattggg cctgaaaatc catacaatac tccagtattt
     3241 gccataaaga aaaaagacag tactaaatgg agaaaattag tagatttcag agaacttaat
     3301 aagagaactc aagatttctg ggaagttcaa ttaggaatac cacatcctgc agggttaaaa
     3361 cagaaaaaat cagtaacagt actggatgtg ggcgatgcat atttttcagt tcccttagat
     3421 aaagacttca ggaagtatac tgcatttacc atacctagta taaacaatga gacaccaggg
     3481 attagatatc agtacaatgt gcttccacag ggatggaaag gatcaccagc aatattccag
     3541 tgtagcatga caaaaatctt agagcctttt agaaaacaaa atccagacat agtcatctat
     3601 caatacatgg atgatttgta tgtaggatct gacttagaaa tagggcagca tagaacaaaa
     3661 atagaggaac tgagacaaca tctgttgagg tggggattta ccacaccaga caaaaaacat
     3721 cagaaagaac ctccattcct ttggatgggt tatgaactcc atcctgataa atggacagta
     3781 cagcctatag tgctgccaga aaaggacagc tggactgtca atgacataca gaaattagtg
     3841 ggaaaattga attgggcaag tcagatttat gcagggatta aagtaaggca attatgtaaa
     3901 cttcttaggg gaaccaaagc actaacagaa gtagtaccac taacagaaga agcagagcta
     3961 gaactggcag aaaacaggga gattctaaaa gaaccggtac atggagtgta ttatgaccca
     4021 tcaaaagact taatagcaga aatacagaag caggggcaag gccaatggac atatcaaatt
     4081 tatcaagagc catttaaaaa tctgaaaaca ggaaagtatg caagaatgaa gggtgcccac
     4141 actaatgatg tgaaacaatt aacagaggca gtacaaaaaa tagccacaga aagcatagta
     4201 atatggggaa agactcctaa atttaaatta cccatacaaa aggaaacatg ggaagcatgg
     4261 tggacagagt attggcaagc cacctggatt cctgagtggg agtttgtcaa tacccctccc
     4321 ttagtgaagt tatggtacca gttagagaaa gaacccataa taggagcaga aactttctat
     4381 gtagatgggg cagccaatag ggaaactaaa ttaggaaaag caggatatgt aactgacaga
     4441 ggaagacaaa aagttgtccc cctaacggac acaacaaatc agaagactga gttacaagca
     4501 attcatctag ctttgcagga ttcgggatta gaagtaaaca tagtgacaga ctcacaatat
     4561 gcattgggaa tcattcaagc acaaccagat aagagtgaat cagagttagt cagtcaaata
     4621 atagagcagt taataaaaaa ggaaaaagtc tacctggcat gggtaccagc acacaaagga
     4681 attggaggaa atgaacaagt agataaattg gtcagtgctg gaatcaggaa agtactattt
     4741 ttagatggaa tagataaggc ccaagaagaa catgagaaat atcacagtaa ttggagagca
     4801 atggctagtg attttaacct accacctgta gtagcaaaag aaatagtagc cagctgtgat
     4861 aaatgtcagc taaaagggga agccatgcat ggacaagtag actgtagccc aggaatatgg
     4921 cagctagatt gtacacattt agaaggaaaa gttatcttgg tagcagttca tgtagccagt
     4981 ggatatatag aagcagaagt aattccagca gagacagggc aagaaacagc atacttcctc
     5041 ttaaaattag caggaagatg gccagtaaaa acagtacata cagacaatgg cagcaatttc
     5101 accagtacta cagttaaggc cgcctgttgg tgggcgggga tcaagcagga atttggcatt
     5161 ccctacaatc cccaaagtca aggagtaata gaatctatga ataaagaatt aaagaaaatt
     5221 ataggacagg taagagatca ggctgaacat cttaagacag cagtacaaat ggcagtattc
     5281 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5341 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5401 aattttcggg tttattacag ggacagcaga gatccagttt ggaaaggacc agcaaagctc
     5461 ctctggaaag gtgaaggggc agtagtaata caagataata gtgacataaa agtagtgcca
     5521 agaagaaaag caaagatcat cagggattat ggaaaacaga tggcaggtga tgattgtgtg
     5581 gcaagtagac aggatgagga ttaacacatg gaattccgga gcggccgcag gagctttgtt
     5641 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     5701 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     5761 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     5821 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     5881 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     5941 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     6001 aagcttccgc ggaattcacc ccaccagtgc aggctgccta tcagaaagtg gtggctggtg
     6061 tggctaatgc cctggcccac aagtatcact aagctcgctt tcttgctgtc caatttctat
     6121 taaaggttcc tttgttccct aagtccaact actaaactgg gggatattat gaagggcctt
     6181 gagcatctgg attctgccta ataaaaaaca tttattttca ttgcaatgat gtatttaaat
     6241 tatttctgaa tattttacta aaaagggaat gtgggaggtc agtgcattta aaacataaag
     6301 aaatgaagag ctagttcaaa ccttgggaaa atacactata tcttaaactc catgaaagaa
     6361 ggtgaggctg caaacagcta atgcacattg gcaacagccc ctgatgccta tgccttattc
     6421 atccctcaga aaaggattca agtagaggct tgatttggag gttaaagttt tgctatgctg
     6481 tattttacat tacttattgt tttagctgtc ctcatgaatg tcttttcact acccatttgc
     6541 ttatcctgca tctctcagcc ttgactccac tcagttctct tgcttagaga taccaccttt
     6601 cccctgaagt gttccttcca tgttttacgg cgagatggtt tctcctcgcc tggccactca
     6661 gccttagttg tctctgttgt cttatagagg tctacttgaa gaaggaaaaa cagggggcat
     6721 ggtttgactg tcctgtgagc ccttcttccc tgcctccccc actcacagtg acccggaatc
     6781 cctcgacatg gcagtctagc actagtgcgg ccgcagatct gcttcctcgc tcactgactc
     6841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     7561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     7621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     7681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     7741 aacttggtct gacagtcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     7801 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     7861 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     7921 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     7981 atcgccatgg gtcacgacga gatcctcgcc gtcgggcatg ctcgccttga gcctggcgaa
     8041 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     8101 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     8161 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     8221 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     8281 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     8341 cagccacgat agccgcgctg cctcgtcttg cagttcattc agggcaccgg acaggtcggt
     8401 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     8461 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     8521 acctgcgtgc aatccatctt gttcaatcat actcttcctt tttcaatatt attgaagcat
     8581 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8641 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtggatc ccctgagggg
     8701 gcccccatgg gctagaggat ccggcctcgg cctctgcata aataaaaaaa attagtcagc
     8761 catgagcttg gcccattgca tacgttgtat ccatatcata atatgtacat ttatattggc
     8821 tcat
//
