LOCUS       62056_16690       13557 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13557)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(241..257)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    460..484
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             1785..2411
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2843..3913
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3982..4176
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    4520..4660
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4846..5434)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5524..6315)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    7939..7963
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8041..8215)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8259..9281)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9348..10024)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10215..10236
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10251..10281
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10289..10305
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10313..10329
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(10346..10598)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(10618..10635)
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(10636..11349)
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMVELYK"
     protein_bind    11396..11520
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(11564..11866)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(12211..12867)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(12921..12951)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(12976..13100)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    13103..13158
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(13189..13533)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
ORIGIN
        1 aacgatggcc tttcctttat cgcaatgatg gcatttgtag gagccacctt ccttttccac
       61 tatcttcaca ataaagtgac agatagctgg gcaatggaat ccgaggaggt ttccggatat
      121 taccctttgt tgaaaagtct caattgccct ttggtcttct gagactgtat ctttgatatt
      181 tttggagtag acaagtgtgt cgtgctccac catgttgacc tgcaggcatg caagcttggc
      241 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      301 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      361 cccttcccaa cagttgcgca gcctgaatgg cgaatgctag agcagcttga gcttggatca
      421 gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat attggcgggt
      481 aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg
      541 tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag
      601 tactttgatc caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc
      661 gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc
      721 cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta
      781 gaaccggaga cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg
      841 cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg
      901 caccaagctg ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag
      961 gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc
     1021 ccgcagcacc cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct
     1081 gcgtagcctg gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac
     1141 cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg
     1201 gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc
     1261 acagatcgcg cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc
     1321 tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt
     1381 gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga
     1441 cgccctggcg gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac
     1501 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg
     1561 tacgtgttcg agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt
     1621 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     1681 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     1741 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     1801 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     1861 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     1921 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     1981 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
     2041 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
     2101 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
     2161 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
     2221 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
     2281 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
     2341 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
     2401 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
     2461 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
     2521 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
     2581 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
     2641 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
     2701 gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa
     2761 cgggcggttg gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa
     2821 cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg
     2881 cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca
     2941 acgcatcgag gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg
     3001 caaagaatcc cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg
     3061 cgacgagcaa ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg
     3121 cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt
     3181 gatccgctac gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc
     3241 cagtgtgtgg gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa
     3301 ccgataccgg gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga
     3361 cgtactcaag ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac
     3421 ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg
     3481 ccgcctggtg acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag
     3541 cgaaaccggg cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat
     3601 cacagaaggc aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc
     3661 cggcatcggc cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag
     3721 atggttgttc aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg
     3781 tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga
     3841 ggcggggcag gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc
     3901 atccgccggt tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa
     3961 aggtcgaaaa ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat
     4021 tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat
     4081 gtaagtgact gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact
     4141 tattaaaact cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga
     4201 agagctgcaa aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg
     4261 tcggcctatc gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc
     4321 agggcgcgga caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct
     4381 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     4441 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     4501 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
     4561 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga
     4621 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct
     4681 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4741 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     4801 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     4861 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     4921 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     4981 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     5041 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     5101 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     5161 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     5221 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     5281 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     5341 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     5401 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     5461 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta
     5521 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     5581 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     5641 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     5701 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     5761 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     5821 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     5881 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     5941 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     6001 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     6061 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     6121 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     6181 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     6241 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     6301 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     6361 gaagctaagg aaggtgcgaa caagtccctg atatgagatc atgtttgtca tctggagcca
     6421 tagaacaggg ttcatcatga gtcatcaact taccttcgcc gacagtgaat tcagcagtaa
     6481 gcgccgtcag accagaaaag agattttctt gtcccgcatg gagcagattc tgccatggca
     6541 aaacatggtg gaagtcatcg agccgtttta ccccaaggct ggtaatggcc ggcgacctta
     6601 tccgctggaa accatgctac gcattcactg catgcagcat tggtacaacc tgagcgatgg
     6661 cgcgatggaa gatgctctgt acgaaatcgc ctccatgcgt ctgtttgccc ggttatccct
     6721 ggatagcgcc ttgccggacc gcaccaccat catgaatttc cgccacctgc tggagcagca
     6781 tcaactggcc cgccaattgt tcaagaccat caatcgctgg ctggccgaag caggcgtcat
     6841 gatgactcaa ggcaccttgg tcgatgccac catcattgag gcacccagct cgaccaagaa
     6901 caaagagcag caacgcgatc cggagatgca tcagaccaag aaaggcaatc agtggcactt
     6961 tggcatgaag gcccacattg gtgtcgatgc caagagtggc ctgacccaca gcctggtcac
     7021 caccgcggcc aacgagcatg acctcaatca gctgggtaat ctgctgcatg gagaggagca
     7081 atttgtctca gccgatgccg gctaccaagg ggcgccacag cgcgaggagc tggccgaggt
     7141 ggatgtggac tggctgatcg ccgagcgccc cggcaaggta agaaccttga aacagcatcc
     7201 acgcaagaac aaaacggcca tcaacatcga atacatgaaa gccagcatcc gggccagggt
     7261 ggagcaccca tttcgcatca tcaagcgaca gttcggcttc gtgaaagcca gatacaaggg
     7321 gttgctgaaa aacgataacc aactggcgat gttattcacg ctggccaacc tgtttcgggc
     7381 ggaccaaatg atacgtcagt gggagagatc tcactaaaaa ctggggataa cgccttaaat
     7441 ggcgaagaaa cggtctaaat aggctgattc aaggcattta cgggagaaaa aatcggctca
     7501 aacatgaaga aatgaaatga ctgagtcagc cgagaagaat ttccccgctt attcgcacct
     7561 tccctaatta taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat
     7621 accagaaaac agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg
     7681 agccgatttt gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac
     7741 atgctaccct ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc
     7801 gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc
     7861 gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg
     7921 gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga cgcttagaca
     7981 acttaataac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
     8041 gatctggatt ttagtactgg attttggttt taggaattag aaattttatt gatagaagta
     8101 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     8161 tataggaacc ctaattccct tatctgggaa ctactcacac attattatgg agaaactcga
     8221 gcttgtcgat cgacagatcc cggtcggcat ctactctatt tctttgccct cggacgagtg
     8281 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
     8341 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
     8401 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
     8461 agttggtcaa gaccaatgcg gagcatatac gcccggagtc gtggcgatcc tgcaagctcc
     8521 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     8581 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     8641 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     8701 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     8761 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     8821 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     8881 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatagc ctccgcgacc
     8941 ggttgtagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgaa
     9001 cggcgggaga tgcaataggt caggctctcg ctaaactccc caatgtcaag cacttccgga
     9061 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     9121 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     9181 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
     9241 aacttctcga cagacgtcgc ggtgagttca ggctttttca tatctcattg ccccccggat
     9301 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
     9361 tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt
     9421 gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt
     9481 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     9541 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     9601 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     9661 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     9721 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttcacatca
     9781 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     9841 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     9901 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     9961 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
    10021 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
    10081 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
    10141 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    10201 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    10261 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    10321 agctatgaca tgattacgaa ttcccgatct agtaacatag atgacaccgc gcgcgataat
    10381 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
    10441 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
    10501 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
    10561 aatcttaaga aactttattg ccaaatgttt gaacgatcgg ggaaattcga gctcttagtg
    10621 gtggtggtgg tggtgtttgt atagttctac catgccatgt gtaatcccag cagctgttac
    10681 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
    10741 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
    10801 ttgataatga tcagcgagtt gcacgccgcc gtcttcgatg ttgtggcggg tcttgaagtt
    10861 ggctttgatg ccgttctttt gcttgtcggc catgatgtat acgttgtggg agttgtagtt
    10921 gtattccaac ttgtggccga ggatgtttcc gtcctccttg aaatcgattc ccttaagctc
    10981 gatcctgttg acgagggtgt ctccctcaaa cttgacttca gcacgtgtct tgtagttccc
    11041 gtcgtccttg aagaagatgg tcctctcctg cacgtatccc tcaggcatgg cgctcttgaa
    11101 gaagtcgtgc cgcttcatat gatcagggta acgggagaag cactgcacgc cgtaggtcag
    11161 ggtggtgacc agggttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag
    11221 ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtgccc
    11281 attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc
    11341 tttactcatt ttttctaccg gtaccgggcc ccccctcgag gcgcgccaag ctatcaccac
    11401 tttgtacaag aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
    11461 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
    11521 gtcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc cagaacatca
    11581 ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact tcttccccga
    11641 taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt tcatccccga
    11701 tatgcaccac cgggtaaagt tcacgggaga ctttatctga cagcagacgt gcactggcca
    11761 gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca tccacaaaca
    11821 gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca ccagcccctg
    11881 ttctcgtcag caaaagagcc gttcatttca ataaaccggg cgacctcagc catcccttcc
    11941 tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg catggttgtg
    12001 cttaccagac cggagatatt gacatcatat atgccttgag caactgatag ctgtcgctgt
    12061 caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac ttcgggtata
    12121 catatcagta tatattctta taccgcaaaa atcagcgcgc aaatacgcat actgttatct
    12181 ggcttttagt aagccggatc cacgcgttta cgccccgccc tgccactcat cgcagtactg
    12241 ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg
    12301 aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac
    12361 gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca
    12421 gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt
    12481 ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg
    12541 gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg
    12601 gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga attccggatg
    12661 agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt
    12721 ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg
    12781 agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt
    12841 ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgacgg
    12901 atcctaactc aaaatccaca cattatacga gccggaagca taaagtgtaa agcctggggt
    12961 gcctaatgcg gccgccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
    13021 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
    13081 gcttttttgt acaaacttgt gataattcga gctcggtacc cggggatcct ctagagtcga
    13141 cctgcaggca tgcaagctaa ttcttaatta actagttcta gagtcgaggt cctctccaaa
    13201 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
    13261 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
    13321 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
    13381 gaggcatctt caacgatggc ctttccttta tcgcaatgat ggcatttgta ggagccacct
    13441 tccttttcca ctatcttcac aataaagtga cagatagctg ggcaatggaa tccgaggagg
    13501 tttccggata ttaccctttg ttgaaaagtc tcaattgccc tttggtcttc tgagact
//
