LOCUS       62056_16685       12460 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12460)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..12460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6..253)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    317..441
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(485..787)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1132..1788)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1842..1872)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(1897..2021)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2040..2753)
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(2791..3135)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    3781..3805
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5105..5731
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6168..7232
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7301..7495
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7839..7979
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8165..8753)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8843..9634)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    10059..10083
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10161..10335)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10378..11400)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11468..12145)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    12336..12357
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12372..12402
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12410..12426
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12434..12450
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
       61 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      121 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      181 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      241 tgtttgaacg atcggggaaa ttcgagctcc accgcggtgg cggccgctct agaactagtt
      301 aattaagaat tatcgaacca ctttgtacaa gaaagctgaa cgagaaacgt aaaatgatat
      361 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      421 acaacatatc cagtcactat ggtcgacctg cagactggct gtgtataagg gagcctgaca
      481 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
      541 gatcagccac ttcttccccg ataacggaga ccggcacact ggccatatcg gtggtcatca
      601 tgcgccagct ttcatccccg atatgcacca ccgggtaaag ttcacgggag actttatctg
      661 acagcagacg tgcactggcc agggggatca ccatccgtcg cccgggcgtg tcaataatat
      721 cactctgtac atccacaaac agacgataac ggctctctct tttataggtg taaaccttaa
      781 actgcatttc accagtccct gttctcgtca gcaaaagagc cgttcatttc aataaaccgg
      841 gcgacctcag ccatcccttc ctgattttcc gctttccagc gttcggcacg cagacgacgg
      901 gcttcattct gcatggttgt gcttaccaga ccggagatat tgacatcata tatgccttga
      961 gcaactgata gctgtcgctg tcaactgtca ctgtaatacg ctgcttcata gcacacctct
     1021 ttttgacata cttcgggtat acatatcagt atatattctt ataccgcaaa aatcagcgcg
     1081 caaatacgca tactgttatc tggcttttag taagccggat cctctagatt acgccccgcc
     1141 ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc
     1201 acagacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata
     1261 atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc
     1321 aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc
     1381 tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag
     1441 aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc
     1501 atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat
     1561 tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa taaaggccgg
     1621 ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat ccagctgaac
     1681 ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg
     1741 ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt tagcttcctt
     1801 agctcctgaa aatctcgccg gatcctaact caaaatccac acattatacg agccggaagc
     1861 ataaagtgta aagcctgggg tgcctaatgc ggccgccata gtgactggat atgttgtgtt
     1921 ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt gatatttata
     1981 tcattttacg tttctcgttc agcttttttg tacaaacttg tttgatagct tggcgcgcct
     2041 ttgtatagtt catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc
     2101 atgtggtctc tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg
     2161 ttgtctggta aaaggacagg gccatcgcca attggagtat tttgttgata atgatcagcg
     2221 agttgcacgc cgccgtcttc gatgttgtgg cgggtcttga agttggcttt gatgccgttc
     2281 ttttgcttgt cggccatgat gtatacgttg tgggagttgt agttgtattc caacttgtgg
     2341 ccgaggatgt ttccgtcctc cttgaaatcg attcccttaa gctcgatcct gttgacgagg
     2401 gtgtctccct caaacttgac ttcagcacgt gtcttgtagt tcccgtcgtc cttgaagaag
     2461 atggtcctct cctgcacgta tccctcaggc atggcgctct tgaagaagtc gtgccgcttc
     2521 atatgatcag ggtaacggga gaagcactgc acgccgtagg tcagggtggt gaccagggtt
     2581 ggccatggaa caggtagttt tccagtagtg caaataaatt taagggtaag ttttccgtat
     2641 gttgcatcac cttcaccctc tccactgaca gaaaatttgt gcccattaac atcaccatct
     2701 aattcaacaa gaattgggac aactccagtg aaaagttctt ctcctttact cattttttct
     2761 accggtaccc ggggatcctc tagagtcgag gtcctctcca aatgaaatga acttccttat
     2821 atagaggaag ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag
     2881 atatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg
     2941 ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttcaacgatg
     3001 gcctttcctt tatcgcaatg atggcatttg taggagccac cttccttttc cactatcttc
     3061 acaataaagt gacagatagc tgggcaatgg aatccgagga ggtttccgga tattaccctt
     3121 tgttgaaaag tctcaattgc cctttggtct tctgagactg tatctttgat atttttggag
     3181 tagacaagtg tgtcgtgctc caccatgtta tcacatcaat ccacttgctt tgaagacgtg
     3241 gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc tttgggacca
     3301 ctgtcggcag aggcatcttc aacgatggcc tttcctttat cgcaatgatg gcatttgtag
     3361 gagccacctt ccttttccac tatcttcaca ataaagtgac agatagctgg gcaatggaat
     3421 ccgaggaggt ttccggatat taccctttgt tgaaaagtct caattgccct ttggtcttct
     3481 gagactgtat ctttgatatt tttggagtag acaagtgtgt cgtgctccac catgttgacc
     3541 tgcaggcacg ccaagcttgg cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3601 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3661 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatgcta
     3721 gagcagcttg agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt
     3781 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata
     3841 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
     3901 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
     3961 ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt cgcacaagtc ctaagttacg
     4021 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
     4081 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
     4141 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
     4201 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
     4261 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg
     4321 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
     4381 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
     4441 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
     4501 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
     4561 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
     4621 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
     4681 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
     4741 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
     4801 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
     4861 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc
     4921 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
     4981 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
     5041 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
     5101 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
     5161 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
     5221 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
     5281 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
     5341 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
     5401 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
     5461 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
     5521 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
     5581 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
     5641 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
     5701 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     5761 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     5821 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     5881 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     5941 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     6001 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     6061 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     6121 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat
     6181 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
     6241 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
     6301 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
     6361 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
     6421 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
     6481 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
     6541 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
     6601 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
     6661 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
     6721 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
     6781 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
     6841 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
     6901 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
     6961 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
     7021 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
     7081 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
     7141 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
     7201 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
     7261 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg
     7321 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
     7381 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
     7441 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
     7501 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
     7561 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
     7621 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
     7681 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     7741 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     7801 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
     7861 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
     7921 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     7981 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     8041 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     8101 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     8161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     8221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8341 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8461 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8521 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8581 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     8641 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8701 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8761 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8821 tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct
     8881 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
     8941 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
     9001 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
     9061 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
     9121 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
     9181 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
     9241 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
     9301 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
     9361 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
     9421 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
     9481 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
     9541 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
     9601 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
     9661 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
     9721 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
     9781 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct
     9841 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
     9901 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
     9961 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
    10021 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
    10081 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt
    10141 aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt taggaattag
    10201 aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc
    10261 tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac
    10321 attattatgg agaaactcga gcttgtcgat cgacagatcc ggtcggcatc tactctattt
    10381 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
    10441 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
    10501 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
    10561 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg
    10621 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
    10681 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
    10741 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
    10801 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
    10861 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
    10921 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
    10981 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
    11041 atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg
    11101 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc taaactcccc
    11161 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
    11221 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
    11281 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
    11341 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
    11401 atctcattgc cccccgggat ctgcgaaagc tcgagagaga tagatttgta gagagagact
    11461 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaaggtcttg
    11521 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
    11581 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
    11641 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
    11701 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
    11761 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
    11821 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
    11881 ctccaccatg ttatcacatc aatccacttg ctttgaagac gtggttggaa cgtcttcttt
    11941 ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg cagaggcatc
    12001 ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac cttccttttc
    12061 tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga ggtttcccga
    12121 tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg tatctttgat
    12181 attcttggag tagacgagag tgtcgtgctc caccatgttg gcaagctgct ctagccaata
    12241 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
    12301 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
    12361 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
    12421 taacaatttc acacaggaaa cagctatgac catgattacg
//
