LOCUS       62056_16680       11752 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11752)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6..253)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    319..443
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(487..789)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1134..1790)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(1844..1874)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(1899..2023)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2083..2427)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    3073..3097
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4397..5023
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5460..6524
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6593..6787
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7131..7271
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7457..8045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8135..8926)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9351..9375
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9453..9627)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9670..10692)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(10760..11437)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11628..11649
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11664..11694
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11702..11718
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11726..11742
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
       61 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      121 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      181 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      241 tgtttgaacg atcggggaaa ttcgagctcc accgcggtgg gcggccgctc tagaactagt
      301 taattaagga attatcgaac cactttgtac aagaaagctg aacgagaaac gtaaaatgat
      361 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
      421 acacaacata tccagtcact atggtcgacc tgcagactgg ctgtgtataa gggagcctga
      481 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
      541 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
      601 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc
      661 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
      721 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
      781 aaactgcatt tcaccagtcc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
      841 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac
      901 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt
      961 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcacacct
     1021 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
     1081 cgcaaatacg catactgtta tctggctttt agtaagccgg atcctctaga ttacgccccg
     1141 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     1201 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     1261 taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     1321 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     1381 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     1441 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     1501 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     1561 attgccatac ggaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc
     1621 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga
     1681 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga
     1741 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc
     1801 ttagctcctg aaaatctcgc cggatcctaa ctcaaaatcc acacattata cgagccggaa
     1861 gcataaagtg taaagcctgg ggtgcctaat gcggccgcca tagtgactgg atatgttgtg
     1921 ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta
     1981 tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgtttgatag cttggcgcgc
     2041 ctcgaggggg ggcccggtac ccggggatcc tctagagtcg aggtcctctc caaatgaaat
     2101 gaacttcctt atatagagga agggtcttgc gaaggatagt gggattgtgc gtcatccctt
     2161 acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg aacgtcttct
     2221 ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc ggcagaggca
     2281 tcttcaacga tggcctttcc tttatcgcaa tgatggcatt tgtaggagcc accttccttt
     2341 tccactatct tcacaataaa gtgacagata gctgggcaat ggaatccgag gaggtttccg
     2401 gatattaccc tttgttgaaa agtctcaatt gccctttggt cttctgagac tgtatctttg
     2461 atatttttgg agtagacaag tgtgtcgtgc tccaccatgt tatcacatca atccacttgc
     2521 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2581 tctttgggac cactgtcggc agaggcatct tcaacgatgg cctttccttt atcgcaatga
     2641 tggcatttgt aggagccacc ttccttttcc actatcttca caataaagtg acagatagct
     2701 gggcaatgga atccgaggag gtttccggat attacccttt gttgaaaagt ctcaattgcc
     2761 ctttggtctt ctgagactgt atctttgata tttttggagt agacaagtgt gtcgtgctcc
     2821 accatgttga cctgcaggca cgccaagctt ggcactggcc gtcgttttac aacgtcgtga
     2881 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     2941 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3001 tggcgaatgc tagagcagct tgagcttgga tcagattgtc gtttcccgcc ttcagtttaa
     3061 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     3121 gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt tgtatgtgca
     3181 tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct ccgctgctat
     3241 agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat gtcgcacaag
     3301 tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct tgtcgcgtgt
     3361 tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc catgaacaag
     3421 agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca ggacttgacc
     3481 aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga gaagatcacc
     3541 ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg ccctggcgac
     3601 gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct actggacatt
     3661 gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc gtgggccgac
     3721 accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc cgagttcgag
     3781 cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc ccgaggcgtg
     3841 aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg cgagctgatc
     3901 gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca tcgctcgacc
     3961 ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag gcggcgcggt
     4021 gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga gaatgaacgc
     4081 caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt ttcattaccg
     4141 aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc gcgcacgtct
     4201 caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg gcggcctggc
     4261 cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     4321 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     4381 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     4441 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     4501 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     4561 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     4621 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     4681 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4741 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     4801 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     4861 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     4921 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4981 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     5041 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     5101 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     5161 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     5221 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     5281 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     5341 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     5401 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgacg
     5461 gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga
     5521 agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg
     5581 aatcgtggca agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg
     5641 gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga
     5701 tgctctatga cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc
     5761 tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg
     5821 tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga
     5881 tggcggtttc ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc
     5941 ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg
     6001 gcggaaagca gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg
     6061 ccatgcagcg tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag
     6121 ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga
     6181 tcgagctagc tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga
     6241 cggttcaccc cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg
     6301 cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca
     6361 gtggcagcgc cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa
     6421 atgacctgcc ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca
     6481 tgcgctaccg caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga
     6541 tgctagggca aattgcccta gcaggggaaa aaggtcgaaa aggtctcttt cctgtggata
     6601 gcacgtacat tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc
     6661 caaagccgta cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag
     6721 gcgatttttc cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct
     6781 gtgcataact gtctggccag cgcacagccg aagagctgca aaaagcgcct acccttcggt
     6841 cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa
     6901 aaatggctgg cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac
     6961 tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa
     7021 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     7081 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     7141 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga
     7201 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7261 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7321 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     7381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     7441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7861 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     7921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8101 gttaagggat tttggtcatg cattctaggt actaaaacaa ttcatccagt aaaatataat
     8161 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
     8221 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
     8281 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
     8341 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
     8401 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
     8461 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
     8521 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
     8581 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
     8641 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
     8701 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
     8761 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
     8821 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
     8881 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
     8941 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
     9001 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
     9061 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
     9121 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
     9181 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
     9241 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
     9301 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg tggcaggata
     9361 tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg gacgttttta
     9421 atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact ggattttggt
     9481 tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac atactaaggg
     9541 tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc cttatctggg
     9601 aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat ccggtcggca
     9661 tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag
     9721 tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc
     9781 gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc
     9841 atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata
     9901 cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg
     9961 ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct cgtattggga
    10021 atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag
    10081 gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc
    10141 ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt cgtccatcac
    10201 agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt
    10261 gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc
    10321 cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca gttcggtttc
    10381 aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc
    10441 gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg
    10501 ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc
    10561 acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag
    10621 gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc
    10681 aggctttttc atatctcatt gccccccggg atctgcgaaa gctcgagaga gatagatttg
    10741 tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt ccttatatag
    10801 aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
    10861 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
    10921 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
    10981 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
    11041 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
    11101 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
    11161 gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag acgtggttgg
    11221 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
    11281 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
    11341 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
    11401 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
    11461 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tggcaagctg
    11521 ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    11581 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    11641 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    11701 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cg
//
