LOCUS       40924_30435       12097 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway destination vector pMDC32-HPB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12097)
  AUTHORS   Qi Y, Katagiri F.
  TITLE     Purification of low-abundance Arabidopsis plasma-membrane protein 
            complexes and identification of candidate components
  JOURNAL   Plant J. 57 (5), 932-944 (2009)
  PUBMED    19000159
REFERENCE   2  (bases 1 to 12097)
  AUTHORS   Qi Y, Katagiri F.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-AUG-2008) Plant Biology, University of Minnesota, 1500
            Gortner Ave., St.Paul, MN 55414, USA
REFERENCE   3  (bases 1 to 12097)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12097)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2009"; volume: "57"; issue: "5"; pages: "932-944"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-AUG-2008) Plant Biology, University of Minnesota, 1500 Gortner 
            Ave., St.Paul, MN 55414, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(6..253)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(576..599)
                     /codon_start=1
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
                     /translation="LEVLFQGP"
     CDS             complement(609..635)
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     protein_bind    664..788
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(832..1134)
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1479..2135)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     promoter        complement(2189..2219)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(2244..2368)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2428..2772)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(3199..3215)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3418..3442
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4742..5368
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5805..6869
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      6938..7132
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7476..7616
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7802..8390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8480..9271)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9696..9720
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(9798..9972)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10015..11037)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11105..11782)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    11973..11994
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12009..12039
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12047..12063
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12071..12087
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aattcagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
       61 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
      121 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
      181 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
      241 tgtttgaacg atcggggaaa ttcgagctcc accgcggtgg gcggccgctc tagaactagt
      301 taattaagga attattaccc tttgatcctg aatagagcac tgccatctga aacctgttgg
      361 cctgctttaa ctttgaggtc ctgtatgctt ccagaggatg gtgctttcac aacgtgctcc
      421 atcttcattg cctctaggac taatataggt tgaccttgat ctactttggc ttcgttttcg
      481 acaaggacct tgactaccaa accagccatg ggggccacaa tggttcctgg agggtgtgat
      541 gatgtttcgg agctggttgg atctgcggaa cccagtggac cctggaacaa aacttcgagg
      601 ccaccggatg cataatcagg aacgtcataa gggtaagaac ctgtgcggcc gccgctagct
      661 cgaaccactt tgtacaagaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
      721 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
      781 tcactatggt cgacctgcag actggctgtg tataagggag cctgacattt atattcccca
      841 gaacatcagg ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc
      901 ttccccgata acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc
      961 atccccgata tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc
     1021 actggccagg gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc
     1081 cacaaacaga cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc
     1141 agtccctgtt ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca
     1201 tcccttcctg attttccgct ttccagcgtt cggcacgcag acgacgggct tcattctgca
     1261 tggttgtgct taccagaccg gagatattga catcatatat gccttgagca actgatagct
     1321 gtcgctgtca actgtcactg taatacgctg cttcatagca cacctctttt tgacatactt
     1381 cgggtataca tatcagtata tattcttata ccgcaaaaat cagcgcgcaa atacgcatac
     1441 tgttatctgg cttttagtaa gccggatcct ctagattacg ccccgccctg ccactcatcg
     1501 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca gacggcatga
     1561 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
     1621 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
     1681 ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag
     1741 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
     1801 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
     1861 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacggaat
     1921 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
     1981 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
     2041 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
     2101 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
     2161 ctcgccggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
     2221 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
     2281 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
     2341 ctcgttcagc ttttttgtac aaacttgttt gatagcttgg cgcgcctcga gggggggccc
     2401 ggtacccggg gatcctctag agtcgaggtc ctctccaaat gaaatgaact tccttatata
     2461 gaggaagggt cttgcgaagg atagtgggat tgtgcgtcat cccttacgtc agtggagata
     2521 tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc cacgatgctc
     2581 ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttc aacgatggcc
     2641 tttcctttat cgcaatgatg gcatttgtag gagccacctt ccttttccac tatcttcaca
     2701 ataaagtgac agatagctgg gcaatggaat ccgaggaggt ttccggatat taccctttgt
     2761 tgaaaagtct caattgccct ttggtcttct gagactgtat ctttgatatt tttggagtag
     2821 acaagtgtgt cgtgctccac catgttatca catcaatcca cttgctttga agacgtggtt
     2881 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     2941 tcggcagagg catcttcaac gatggccttt cctttatcgc aatgatggca tttgtaggag
     3001 ccaccttcct tttccactat cttcacaata aagtgacaga tagctgggca atggaatccg
     3061 aggaggtttc cggatattac cctttgttga aaagtctcaa ttgccctttg gtcttctgag
     3121 actgtatctt tgatattttt ggagtagaca agtgtgtcgt gctccaccat gttgacctgc
     3181 aggcacgcca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag
     3361 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
     3421 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt
     3481 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     3541 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
     3601 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     3661 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     3721 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     3781 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     3841 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     3901 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     3961 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     4021 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     4081 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     4141 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     4201 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     4261 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     4321 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     4381 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     4441 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     4501 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     4561 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     4621 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     4681 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     4741 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     4801 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     4861 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     4921 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     4981 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     5041 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     5101 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     5161 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     5221 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     5281 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     5341 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     5401 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     5461 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     5521 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     5581 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     5641 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     5701 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     5761 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
     5821 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     5881 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     5941 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     6001 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     6061 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     6121 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     6181 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     6241 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     6301 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     6361 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     6421 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     6481 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     6541 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     6601 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     6661 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     6721 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     6781 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     6841 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     6901 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     6961 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     7021 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     7081 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     7141 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     7201 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     7261 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     7321 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     7381 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     7441 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     7501 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     7561 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     7621 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7681 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8221 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8461 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     8521 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     8581 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     8641 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
     8701 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
     8761 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
     8821 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
     8881 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
     8941 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
     9001 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
     9061 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
     9121 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
     9181 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
     9241 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
     9301 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
     9361 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
     9421 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
     9481 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
     9541 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
     9601 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
     9661 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
     9721 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
     9781 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
     9841 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
     9901 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
     9961 attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt
    10021 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
    10081 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
    10141 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
    10201 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg
    10261 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
    10321 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
    10381 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
    10441 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
    10501 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
    10561 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
    10621 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
    10681 catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa
    10741 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat
    10801 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
    10861 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
    10921 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
    10981 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
    11041 tcattgcccc ccgggatctg cgaaagctcg agagagatag atttgtagag agagactggt
    11101 gatttcagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa ggtcttgcga
    11161 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
    11221 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
    11281 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
    11341 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
    11401 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
    11461 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
    11521 caccatgtta tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    11581 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    11641 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    11701 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    11761 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    11821 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
    11881 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    11941 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    12001 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    12061 caatttcaca caggaaacag ctatgaccat gattacg
//
