LOCUS       62056_16675        6443 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          718..1193
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1267..1974
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             2008..3540
                     /codon_start=1
                     /label=VSV-G
                     /note="vesicular stomatitis virus G glycoprotein"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3625..4019
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(4529..5117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5291..6148)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6149..6253)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccc ctcgaagctt acatgtggta ccgagctcgg atcctgagaa cttcagggtg
      721 agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat
      781 aggaagggga gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt
      841 aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact
      901 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca
      961 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa
     1021 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac
     1081 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc
     1141 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg
     1201 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agcacgtgag atctgaattc
     1261 gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     1321 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     1381 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     1441 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     1501 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     1561 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     1621 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     1681 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     1741 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     1801 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     1861 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1921 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtccgga
     1981 ctcagatctc gaggcggcgg agggagtatg aagtgccttt tgtacttagc ctttttattc
     2041 attggggtga attgcaagtt caccatagtt tttccacaca accaaaaagg aaactggaaa
     2101 aatgttcctt ctaattacca ttattgcccg tcaagctcag atttaaattg gcataatgac
     2161 ttaataggca cagccttaca agtcaaaatg cccaagagtc acaaggctat tcaagcagac
     2221 ggttggatgt gtcatgcttc caaatgggtc actacttgtg atttccgctg gtatggaccg
     2281 aagtatataa cacattccat ccgatccttc actccatctg tagaacaatg caaggaaagc
     2341 attgaacaaa cgaaacaagg aacttggctg aatccaggct tccctcctca aagttgtgga
     2401 tatgcaactg tgacggatgc cgaagcagtg attgtccagg tgactcctca ccatgtgctg
     2461 gttgatgaat acacaggaga atgggttgat tcacagttca tcaacggaaa atgcagcaat
     2521 tacatatgcc ccactgtcca taactctaca acctggcatt ctgactataa ggtcaaaggg
     2581 ctatgtgatt ctaacctcat ttccatggac atcaccttct tctcagagga cggagagcta
     2641 tcatccctgg gaaaggaggg cacagggttc agaagtaact actttgctta tgaaactgga
     2701 ggcaaggcct gcaaaatgca atactgcaag cattggggag tcagactccc atcaggtgtc
     2761 tggttcgaga tggctgataa ggatctcttt gctgcagcca gattccctga atgcccagaa
     2821 gggtcaagta tctctgctcc atctcagacc tcagtggatg taagtctaat tcaggacgtt
     2881 gagaggatct tggattattc cctctgccaa gaaacctgga gcaaaatcag agcgggtctt
     2941 ccaatctctc cagtggatct cagctatctt gctcctaaaa acccaggaac cggtcctgct
     3001 ttcaccataa tcaatggtac cctaaaatac tttgagacca gatacatcag agtcgatatt
     3061 gctgctccaa tcctctcaag aatggtcgga atgatcagtg gaactaccac agaaagggaa
     3121 ctgtgggatg actgggcacc atatgaagac gtggaaattg gacccaatgg agttctgagg
     3181 accagttcag gatataagtt tcctttatac atgattggac atggtatgtt ggactccgat
     3241 cttcatctta gctcaaaggc tcaggtgttc gaacatcctc acattcaaga cgctgcttcg
     3301 caacttcctg atgatgagag tttatttttt ggtgatactg ggctatccaa aaatccaatc
     3361 gagcttgtag aaggttggtt cagtagttgg aaaagctcta ttgcctcttt tttctttatc
     3421 atagggttaa tcattggact attcttggtt ctccgagttg gtatccatct ttgcattaaa
     3481 ttaaagcaca ccaagaaaag acagatttat acagacatag agatgaaccg acttggaaag
     3541 taagaattca ccccaccagt gcaggctgcc tatcagaaag tggtggctgg tgtggctaat
     3601 gccctggccc acaagtatca ctaagctcgc tttcttgctg tccaatttct attaaaggtt
     3661 cctttgttcc ctaagtccaa ctactaaact gggggatatt atgaagggcc ttgagcatct
     3721 ggattctgcc taataaaaaa catttatttt cattgcaatg atgtatttaa attatttctg
     3781 aatattttac taaaaaggga atgtgggagg tcagtgcatt taaaacataa agaaatgaag
     3841 agctagttca aaccttggga aaatacacta tatcttaaac tccatgaaag aaggtgaggc
     3901 tgcaaacagc taatgcacat tggcaacagc ccctgatgcc tatgccttat tcatccctca
     3961 gaaaaggatt caagtagagg cttgatttgg aggttaaagt tttgctatgc tgtattttac
     4021 attacttatt gttttagctg tcctcatgaa tgtcttttca ctacccattt gcttatcctg
     4081 catctctcag ccttgactcc actcagttct cttgcttaga gataccacct ttcccctgaa
     4141 gtgttccttc catgttttac ggcgagatgg tttctcctcg cctggccact cagccttagt
     4201 tgtctctgtt gtcttataga ggtctacttg aagaaggaaa aacagggggc atggtttgac
     4261 tgtcctgtga gcccttcttc cctgcctccc ccactcacag tgacccggaa tccctcgaca
     4321 tggcagtcta gcactagtgc ggccgcagat ctgcttcctc gctcactgac tcgctgcgct
     4381 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4441 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4501 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4561 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4621 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4681 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4741 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4801 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4861 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4921 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4981 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     5041 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     5101 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     5161 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5221 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5281 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     5341 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     5401 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     5461 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     5521 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     5581 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     5641 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     5701 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     5761 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     5821 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     5881 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     5941 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     6001 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     6061 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     6121 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     6181 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     6241 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtggatccc ctgagggggc
     6301 ccccatgggc tagaggatcc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     6361 tgagcttggc ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc
     6421 atgtccaaca ttaccgccat gtt
//
