LOCUS       62056_16665        6452 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6452)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          718..1193
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1267..1983
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2017..3549
                     /codon_start=1
                     /label=VSV-G
                     /note="vesicular stomatitis virus G glycoprotein"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3634..4028
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(4538..5126)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5300..6157)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6158..6262)
                     /label=AmpR promoter
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccc ctcgaagctt acatgtggta ccgagctcgg atcctgagaa cttcagggtg
      721 agtctatggg acccttgatg ttttctttcc ccttcttttc tatggttaag ttcatgtcat
      781 aggaagggga gaagtaacag ggtacacata ttgaccaaat cagggtaatt ttgcatttgt
      841 aattttaaaa aatgctttct tcttttaata tacttttttg tttatcttat ttctaatact
      901 ttccctaatc tctttctttc agggcaataa tgatacaatg tatcatgcct ctttgcacca
      961 ttctaaagaa taacagtgat aatttctggg ttaaggcaat agcaatattt ctgcatataa
     1021 atatttctgc atataaattg taactgatgt aagaggtttc atattgctaa tagcagctac
     1081 aatccagcta ccattctgct tttattttat ggttgggata aggctggatt attctgagtc
     1141 caagctaggc ccttttgcta atcatgttca tacctcttat cttcctccca cagctcctgg
     1201 gcaacgtgct ggtctgtgtg ctggcccatc actttggcaa agcacgtgag atctgaattc
     1261 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     1321 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     1381 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     1441 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     1501 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1561 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1621 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1681 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     1741 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     1801 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1861 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     1921 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1981 aagtccggac tcagatctcg aggcggcgga gggagtatga agtgcctttt gtacttagcc
     2041 tttttattca ttggggtgaa ttgcaagttc accatagttt ttccacacaa ccaaaaagga
     2101 aactggaaaa atgttccttc taattaccat tattgcccgt caagctcaga tttaaattgg
     2161 cataatgact taataggcac agccttacaa gtcaaaatgc ccaagagtca caaggctatt
     2221 caagcagacg gttggatgtg tcatgcttcc aaatgggtca ctacttgtga tttccgctgg
     2281 tatggaccga agtatataac acattccatc cgatccttca ctccatctgt agaacaatgc
     2341 aaggaaagca ttgaacaaac gaaacaagga acttggctga atccaggctt ccctcctcaa
     2401 agttgtggat atgcaactgt gacggatgcc gaagcagtga ttgtccaggt gactcctcac
     2461 catgtgctgg ttgatgaata cacaggagaa tgggttgatt cacagttcat caacggaaaa
     2521 tgcagcaatt acatatgccc cactgtccat aactctacaa cctggcattc tgactataag
     2581 gtcaaagggc tatgtgattc taacctcatt tccatggaca tcaccttctt ctcagaggac
     2641 ggagagctat catccctggg aaaggagggc acagggttca gaagtaacta ctttgcttat
     2701 gaaactggag gcaaggcctg caaaatgcaa tactgcaagc attggggagt cagactccca
     2761 tcaggtgtct ggttcgagat ggctgataag gatctctttg ctgcagccag attccctgaa
     2821 tgcccagaag ggtcaagtat ctctgctcca tctcagacct cagtggatgt aagtctaatt
     2881 caggacgttg agaggatctt ggattattcc ctctgccaag aaacctggag caaaatcaga
     2941 gcgggtcttc caatctctcc agtggatctc agctatcttg ctcctaaaaa cccaggaacc
     3001 ggtcctgctt tcaccataat caatggtacc ctaaaatact ttgagaccag atacatcaga
     3061 gtcgatattg ctgctccaat cctctcaaga atggtcggaa tgatcagtgg aactaccaca
     3121 gaaagggaac tgtgggatga ctgggcacca tatgaagacg tggaaattgg acccaatgga
     3181 gttctgagga ccagttcagg atataagttt cctttataca tgattggaca tggtatgttg
     3241 gactccgatc ttcatcttag ctcaaaggct caggtgttcg aacatcctca cattcaagac
     3301 gctgcttcgc aacttcctga tgatgagagt ttattttttg gtgatactgg gctatccaaa
     3361 aatccaatcg agcttgtaga aggttggttc agtagttgga aaagctctat tgcctctttt
     3421 ttctttatca tagggttaat cattggacta ttcttggttc tccgagttgg tatccatctt
     3481 tgcattaaat taaagcacac caagaaaaga cagatttata cagacataga gatgaaccga
     3541 cttggaaagt aagaattcac cccaccagtg caggctgcct atcagaaagt ggtggctggt
     3601 gtggctaatg ccctggccca caagtatcac taagctcgct ttcttgctgt ccaatttcta
     3661 ttaaaggttc ctttgttccc taagtccaac tactaaactg ggggatatta tgaagggcct
     3721 tgagcatctg gattctgcct aataaaaaac atttattttc attgcaatga tgtatttaaa
     3781 ttatttctga atattttact aaaaagggaa tgtgggaggt cagtgcattt aaaacataaa
     3841 gaaatgaaga gctagttcaa accttgggaa aatacactat atcttaaact ccatgaaaga
     3901 aggtgaggct gcaaacagct aatgcacatt ggcaacagcc cctgatgcct atgccttatt
     3961 catccctcag aaaaggattc aagtagaggc ttgatttgga ggttaaagtt ttgctatgct
     4021 gtattttaca ttacttattg ttttagctgt cctcatgaat gtcttttcac tacccatttg
     4081 cttatcctgc atctctcagc cttgactcca ctcagttctc ttgcttagag ataccacctt
     4141 tcccctgaag tgttccttcc atgttttacg gcgagatggt ttctcctcgc ctggccactc
     4201 agccttagtt gtctctgttg tcttatagag gtctacttga agaaggaaaa acagggggca
     4261 tggtttgact gtcctgtgag cccttcttcc ctgcctcccc cactcacagt gacccggaat
     4321 ccctcgacat ggcagtctag cactagtgcg gccgcagatc tgcttcctcg ctcactgact
     4381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     4921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     4981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5221 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5281 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5341 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5401 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5461 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5521 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5581 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5641 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5701 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5761 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5821 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     5881 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     5941 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6001 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6061 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6121 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6181 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6241 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtggatcccc
     6301 tgagggggcc cccatgggct agaggatccg gcctcggcct ctgcataaat aaaaaaaatt
     6361 agtcagccat gagcttggcc cattgcatac gttgtatcca tatcataata tgtacattta
     6421 tattggctca tgtccaacat taccgccatg tt
//
