LOCUS       V004803                 6155 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004803
VERSION     V004803
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6155)
  AUTHORS   Dharmasena MN, Hanisch BW, Wai TT, Kopecko DJ.
  TITLE     Stable expression of Shigella sonnei form I O-polysaccharide genes
            recombineered into the chromosome of live Salmonella oral vaccine
            vector Ty21a
  JOURNAL   Int. J. Med. Microbiol. 303 (3), 105-113 (2013)
   PUBMED   23474241
REFERENCE   2  (bases 1 to 6155)
  AUTHORS   Dharmasena MN, Hanisch BW, Wai TT, Kopecko DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2012) Laboratory of Enteric and Sexually
            Transmitted Diseases, FDA-CBER, Lincoln Drive, Bethesda, MD 20892,
            USA
REFERENCE   3  (bases 1 to 6155)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6155)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vertor NTI v. NTI
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Int. J.
            Med. Microbiol."; date: "2013"; volume: "303"; issue: "3"; pages:
            "105-113"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-JUL-2012) Laboratory of Enteric and Sexually Transmitted
            Diseases, FDA-CBER, Lincoln Drive, Bethesda, MD 20892, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             109..1173
                     /gene="vexA"
                     /label="Vi polysaccharide export protein VexA/TviF"
                     /note="Vi polysaccharide export protein VexA/TviF from
                     Salmonella typhi. Accession#: Q04976"
     rep_origin      1658..1880
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             1928..2875
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     gene            3914..4420
                     /gene="tivD"
                     /label="tivD"
     CDS             3914..4417
                     /codon_start=1
                     /gene="tivD"
                     /product="TivD"
                     /label="tivD"
                     /protein_id="AGH89392.1"
                     /translation="PFLIDLIANVMSITLSFQNASMNKLFEKECRNVATRALKYVRQKK
                     TEGRLDEALSVLISLKRIEPDVSRLMREYKQIIRLFNESRKDGGSTITSYEHLDYAKKL
                     LVFDSENAYALKYAALNAMHLRDYTQALQYWQRLEKVNGPTEPVTRQISTCITALQKNT
                     SGKS"
     protein_bind    complement(4444..4491)
                     /label="FRT"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             4852..5643
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(5837..5870)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtcgacgggc ggccgcaagc ttggatccac cggtctcgag gggcgcgccg gtaccgaatt
       61 ctaattaatg ggcatcattt ttcagctatt tcatttataa aataagttat gaaaaaaatc
      121 atcatattac taacgacatt tttcctgctt tcgggatgca ctattcccag ggcggtattt
      181 aaatccagcc ttattaatca ggacgatcct cgttataatc tggtcgaagt cacgccgaca
      241 ttaaaactaa gcgctcccga tactgtgccg aaaactattg tcgatccggt ttttgccgca
      301 aataactggc actggacatc tttggctaaa ggcgatgtgc tgcatatcac tattttatcc
      361 tcgggcgggg ctggatattt atccaataac gcgagcggcg accgtgcgga ttttgaaaat
      421 attcttgtga ctgacagtaa taccgttcag gtgccttatg ccgggacaat cccggtttct
      481 ggattggatg tgacgcaact ggctgatgag atcaaaaagc gactttcgcg cgttgtcctg
      541 aatcctcagg tgattgtgac acttaccgcc cgcaccggag ccatggtgac ggtcgagggc
      601 agcgggaaaa cgggtcgata ccctctcgaa cagagtatga atcgtctgag tcatcttttg
      661 gcaacggcag tggcagtaga aaataccagc acagatatga tggaagttca cgtgacgcgc
      721 cagcagcatt atttcactgc gcgactgtca gatatttatc agtatcccgg attggatatt
      781 gccttacagc cggatgaccg tatcactctg cgtcaagtga ccgagtatgt taatgtgctc
      841 ggcgcagcag gtgttcaggg gaaacatgct ctggttcagc gtcattccag cgtcgtggat
      901 gctttggcgc tcgcgaaagg attaaatgac aatcttgccg atccgcaagc gatttttctg
      961 tacaagcata acgaagcgga acaggcgaaa cagcagatgc gtaagctgaa tatctatcat
     1021 gtcgatatga gccaacctaa ctcggtgttt ttagcgcagg ccatacgagt ggacaacggc
     1081 gatgttatct atatctcgaa cgcctctttg actgatttcg ctaaggtgaa agccgcattc
     1141 gatagctttt tgacccgcgg cactaattct ttctaaccga cagtaagacg ggtaagcctg
     1201 ttgatgatac cgctgcctta ctgggtgcat tagccagtct gaatgacctg tcacgggata
     1261 atccgaagtg gtcagactgg aaaatcagag ggcaggaact gctgaacagc aaaaagtcag
     1321 atagcaccac atagcagacc cgccataaaa cgccctgaga agcccgtgac gggcttttct
     1381 tgtattatgg gtagtttcct tgcatgaatc cataaaaggc gcctgtagtg ccatttaccc
     1441 ccattcactg ccagagccgt gagcgcagcg aactgaatgt cacgaaaaag acagcgactc
     1501 aggtgcctga tggtcggaga caaaaggaat attcagcgat ttgcccgagc ttgcgagggt
     1561 gctacttaag cctttagggt tttaaggtct gttttgtaga ggagcaaaca gcgtttgcga
     1621 catccttttg taatactgcg gaactgacta aagtagtgag ttatacacag ggctgggatc
     1681 tattcttttt atcttttttt attctttctt tattctataa attataacca cttgaatata
     1741 aacaaaaaaa acacacaaag gtctagcgga atttacagag ggtctagcag aatttacaag
     1801 ttttccagca aaggtctagc agaatttaca gatacccaca actcaaagga aaaggactag
     1861 taattatcat tgactagccc atctcaattg gtatagtgat taaaatcacc tagaccaatt
     1921 gagatgtatg tctgaattag ttgttttcaa agcaaatgaa ctagcgatta gtcgctatga
     1981 cttaacggag catgaaacca agctaatttt atgctgtgtg gcactactca accccacgat
     2041 tgaaaaccct acaaggaaag aacggacggt atcgttcact tataaccaat acgctcagat
     2101 gatgaacatc agtagggaaa atgcttatgg tgtattagct aaagcaacca gagagctgat
     2161 gacgagaact gtggaaatca ggaatccttt ggttaaaggc tttgagattt tccagtggac
     2221 aaactatgcc aagttctcaa gcgaaaaatt agaattagtt tttagtgaag agatattgcc
     2281 ttatcttttc cagttaaaaa aattcataaa atataatctg gaacatgtta agtcttttga
     2341 aaacaaatac tctatgagga tttatgagtg gttattaaaa gaactaacac aaaagaaaac
     2401 tcacaaggca aatatagaga ttagccttga tgaatttaag ttcatgttaa tgcttgaaaa
     2461 taactaccat gagtttaaaa ggcttaacca atgggttttg aaaccaataa gtaaagattt
     2521 aaacacttac agcaatatga aattggtggt tgataagcga ggccgcccga ctgatacgtt
     2581 gattttccaa gttgaactag atagacaaat ggatctcgta accgaacttg agaacaacca
     2641 gataaaaatg aatggtgaca aaataccaac aaccattaca tcagattcct acctacataa
     2701 cggactaaga aaaacactac acgatgcttt aactgcaaaa attcagctca ccagttttga
     2761 ggcaaaattt ttgagtgaca tgcaaagtaa gtatgatctc aatggttcgt tctcatggct
     2821 cacgcaaaaa caacgaacca cactagagaa catactggct aaatacggaa ggatctgagg
     2881 ttcttatggc tcttgtatct atcagtgaag catcaagact aacaaacaaa agtagaacaa
     2941 ctgttcaccg ttacatatca aagggaaaac tgtccatatg cacagatgaa aacggtgtaa
     3001 aaaagataga tacatcagag cttttacgag tttttggtgc attcaaagct gttcaccatg
     3061 aacagatcga caatgtaaca gatgaacagc atgtaacacc taatagaaca ggtgaaacca
     3121 gtaaaacaaa gcaactagaa catgaaattg aacacctgag acaacttgtt acagctcaac
     3181 agtcacacat agacagcctg aaacaggcga tgctgcttat cgaatcaaag ctgccgacaa
     3241 cacgggagcc agtgacgcct cccgtgggga aaaaatcatg gcaattctgg aagaaatagc
     3301 gctttcagcc ggcaaacctg aagccggatc tgcgattctg ataacaaact agcaacacca
     3361 gaacagcccg tttgcgggca gcaaaacccg tgggaattaa ttcccctgct cgcgcaggct
     3421 gggtgccaag ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac
     3481 gatgatcgtg ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat
     3541 ccgcatgccc gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc
     3601 gaggatgcga accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg
     3661 tcttccgatc tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag
     3721 caaggccgcc aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca
     3781 ggacagaaat gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa
     3841 acggatgaag gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa
     3901 gtagcgtgct agcccgttcc tcattgattt gattgctaac gtcatgagca ttactctatc
     3961 attccagaat gcctcaatga acaagttgtt tgagaaagag tgtcgcaatg ttgcaaccag
     4021 agcccttaaa tatgtacgcc agaagaaaac tgaagggcgt ctggatgaag cattgtctgt
     4081 attgattagc ctgaaacgaa ttgagcctga tgtttctcgt ctgatgcgtg aatataagca
     4141 aattatcaga ttatttaatg agtcacggaa ggatggcggt agcactatca cgtcttatga
     4201 acatctagac tatgcgaaaa aattactcgt ttttgatagc gaaaatgcct atgccttgaa
     4261 atatgccgca ttaaatgcaa tgcatttacg cgactacacg caggctttgc agtattggca
     4321 gcgactggag aaagtgaatg gaccaacgga gccggtgaca aggcagatct cgacctgcat
     4381 aaccgcatta caaaaaaata catcagggaa gtcgtaaccc ggggtgtagg ctggagctgc
     4441 ttcgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caagatcccc
     4501 tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa
     4561 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca
     4621 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag
     4681 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct
     4741 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga
     4801 tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     4861 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     4921 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     4981 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     5041 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     5101 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     5161 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     5221 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     5281 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     5341 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     5401 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     5461 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     5521 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     5581 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     5641 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     5701 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5761 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5821 gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa
     5881 ctaaggagga tattcatata tgcattaatg tctaacaatt cgttcaagcc gacgccgctt
     5941 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     6001 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     6061 ggagatatat catgaaaggc tggctttttc ttgttatcgc aatagttggc gaagtaatcg
     6121 caacatccgc attaaaatct agcgagggct ttact
//
