LOCUS       V004804                12362 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004804
VERSION     V004804
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12362)
  AUTHORS   Xi J, Wang X, Dong L, Dong Y, Su Y, Tang Q, Wang Z.
  TITLE     Construction of a standard reference plasmid containing seven target
            genes for the detection of transgenic cotton
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12362)
  AUTHORS   Xi J, Wang X, Dong L, Dong Y, Su Y, Tang Q, Wang Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2014) Biosafet Lab, Biotechnology Research
            Institute, No. 12 Zhongguancun South Street, Beijing 100081, China
REFERENCE   3  (bases 1 to 12362)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12362)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (14-APR-2014) Biosafet Lab, Biotechnology Research Institute, No. 12
            Zhongguancun South Street, Beijing 100081, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1219..1845
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             2282..3346
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      3415..3609
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3953..4093
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4279..4867)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4957..5748)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    6173..6197
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(6275..6449)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6509..7297)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(7366..8043)
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    8234..8255
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8270..8300
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8308..8324
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8332..8348
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             8364..8633
                     /codon_start=1
                     /gene="cptI"
                     /product="cowpea trypsin inhibitor"
                     /label="cptI"
                     /protein_id="AII71483.1"
                     /translation="MRMDLKHLGSNHHDDSSDEPSESSEPCCDSCICTKSIPPQCHCTD
                     IRWNSCHSACKSCMCTRSMPGKCRCLDIADFCYKPCKSRDEDDE"
     gene            8364..8633
                     /gene="cptI"
                     /label="cptI"
     CDS             complement(8551..9579)
                     /codon_start=1
                     /gene="Cry1Ab/1Ac"
                     /product="Cry1Ab/Cry1Ac fusion protein"
                     /label="Cry1Ab/1Ac"
                     /note="Bt protein"
                     /protein_id="AII71484.1"
                     /translation="NCLSNPEVVVLGGERIETGYTPIDISLSLTQFLLSEFVPGAGFVL
                     GPVDIIWGIFGPSQWDAFLVQIEQLINQRIEEFARNQAISRLEGLSNLYQIYAESFREW
                     EADPTNPALREEMRIQFNDMNSALTTAIPLFAVQNYQVPLLSVYVQAANLHLSVLRDVS
                     VFGQRWGFDAATINSRYNDLTRLIGNYTDHAVRWYNTGLERVWGPDSRDWIRYNQFRRE
                     LTLTVLDIVSLFPNYDSRTYPIRTVSQLTREIYTNPVLENFDGSFRGSAQGIEGSIRSP
                     HLMDILNSITIYTDAHRGEYYWSGHQIMASPVGFIIRRLRHEICTVCSRSPRCPGNGIY
                     PA"
     gene            complement(8551..9579)
                     /gene="Cry1Ab/1Ac"
                     /label="Cry1Ab/1Ac"
     CDS             9595..10959
                     /gene="aroA"
                     /label="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /note="3-phosphoshikimate 1-carboxyvinyltransferase from
                     Agrobacterium sp. (strain CP4). Accession#: Q9R4E4"
     regulatory      10963..11180
                     /label="NOS"
                     /note="NOS"
                     /regulatory_class="terminator"
     CDS             join(11182..11296,11671..12064)
                     /codon_start=1
                     /gene="SadI"
                     /product="AE9 stearoyl-ACP desaturase"
                     /label="SadI"
                     /note="from cotton K312"
                     /protein_id="AII71486.1"
                     /translation="STMPSPRNLRSSLALLFHQRPPLDLPSFP*SPPFLLAPKRLGI*K
                     SLSRLQRRCLFRSPTPCRLTRLRSLNLWRAGLRTTF*LTSNQLRNVGNPPTFFQILILM
                     DFMSKSKSLGKGQRRSQMITL*FWLVI*SPRKPFQLIKQCLIPWMELVMRQVLASLALA
                     VVLQRR"
     gene            11182..12064
                     /gene="SadI"
                     /label="SadI"
     primer_bind     complement(12038..12054)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    12257..12281
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
       61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
      121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
      181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
      241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
      301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
      361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
      421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
      481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
      541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
      601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
      661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
      721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
      781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
      841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
      901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
      961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
     1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
     4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
     5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
     5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
     5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
     5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
     5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
     5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
     5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
     5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
     5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
     5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
     5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
     5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
     5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
     5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
     5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
     5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
     6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
     6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
     6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
     6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
     6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
     6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
     6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
     6421 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagagg
     6481 atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
     6541 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
     6601 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
     6661 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
     6721 gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
     6781 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
     6841 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
     6901 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
     6961 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
     7021 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
     7081 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc agggcaccgg
     7141 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
     7201 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
     7261 cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat cgacagatct
     7321 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
     7381 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
     7441 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
     7501 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
     7561 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
     7621 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
     7681 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
     7741 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt atcacatcaa
     7801 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt
     7861 gggggtccat ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta
     7921 tcgcaatgat ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga
     7981 cagatagctg ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc
     8041 tcaatagccc tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg
     8101 tcgtgctcca ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc
     8161 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     8221 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     8281 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     8341 gctatgacca tgattacgaa ttcatgcgta tggacctgaa acacctgggt tctaaccacc
     8401 acgacgactc ttctgacgaa ccgtctgaat cttctgaacc gtgctgcgac tcttgcatct
     8461 gcaccaaatc tatcccgccg cagtgccact gcaccgacat ccgttggaac tcttgccact
     8521 ctgcgtgcaa atcttgcatg tgcacccgtt ctatgccggg taaatgccgt tgcctggaca
     8581 tcgcggactt ctgctacaaa ccgtgcaaat ctcgtgacga agacgacgaa taatgaatcc
     8641 aactggagag gccatgatct ggtgtccaga ccagtaatac tctcctctgt gagcatcggt
     8701 gtagatagtt atgctgttca agatgtccat caagtgtggg ctcctgatgg agccttcgat
     8761 accttgggca gaaccgcgga agctaccgtc gaagttctca agaactgggt tagtatagat
     8821 ttctctggta agttgggaca ctgtacggat agggtaggtt ctggagtcat agttcgggaa
     8881 gagagacaca atgtccaaaa ctgtgagggt caattctctc ctgaactggt tgtatctaat
     8941 ccaatctcta gaatcaggac cccagacacg ctccaagcca gtgttgtacc aacgaacagc
     9001 gtggtcggtg tagtttccaa tcagcctagt aaggtcgttg taacggctat tgatggttgc
     9061 agcatcgaat ccccaccttt gcccaaacac gctaacgtct cgaagcacgc tgaggtgaag
     9121 attagctgct tgaacgtaca cggacaagag aggaacttgg tagttctgga ctgcgaacaa
     9181 tgggatagct gtggtcaagg cgctgttcat gtcgttgaat tgaatacgca tttcctcgcg
     9241 gagagctggg ttagtaggat cggcttccca ctctctgaag ctctctgcat agatttggta
     9301 gagattgctc aatccttcca acctagagat ggcctggttc ctggcgaact cttcgatcct
     9361 ctggttgatc aactgctcaa tttgcaccag gaatgcatcc cattgagatg gaccaaagat
     9421 accccagatg atgtcaactg gtccgagaac gaacccagca cctggcacga actcgctgag
     9481 cagaaactgt gtcaaggaca aggagatgtc gatgggagtg taaccggttt caatgcgttc
     9541 tccaccaagt actacaactt ctgggttact caagcagttg gtacccgggg atccatggct
     9601 cacggtgcaa gcagccgtcc agcaactgct cgtaagtcct ctggtctttc tggaaccgtc
     9661 cgtattccag gtgacaagtc tatctcccac aggtccttca tgtttggagg tctcgctagc
     9721 ggtgaaactc gtatcaccgg tcttttggaa ggtgaagatg ttatcaacac tggtaaggct
     9781 atgcaagcta tgggtgccag aatccgtaag gaaggtgata cttggatcat tgatggtgtt
     9841 ggtaacggtg gactccttgc tcctgaggct cctctcgatt tcggtaacgc tgcaactggt
     9901 tgccgtttga ctatgggtct tgttggtgtt tacgatttcg atagcacttt cattggtgac
     9961 gcttctctca ctaagcgtcc aatgggtcgt gtgttgaacc cacttcgcga aatgggtgtg
    10021 caggtgaagt ctgaagacgg tgatcgtctt ccagttacct tgcgtggacc aaagactcca
    10081 acgccaatca cctacagggt acctatggct tccgctcaag tgaagtccgc tgttctgctt
    10141 gctggtctca acaccccagg tatcaccact gttatcgagc caatcatgac tcgtgaccac
    10201 actgaaaaga tgcttcaagg ttttggtgct aaccttaccg ttgagactga tgctgacggt
    10261 gtgcgtacca tccgtcttga aggtcgtggt aagctcaccg gtcaagtgat tgatgttcca
    10321 ggtgatccat cctctactgc tttcccattg gttgctgcct tgcttgttcc aggttccgac
    10381 gtcaccatcc ttaacgtttt gatgaaccca acccgtactg gtctcatctt gactctgcag
    10441 gaaatgggtg ccgacatcga agtgatcaac ccacgtcttg ctggtggaga agacgtggct
    10501 gacttgcgtg ttcgttcttc tactttgaag ggtgttactg ttccagaaga ccgtgctcct
    10561 tctatgatcg acgagtatcc aattctcgct gttgcagctg cattcgctga aggtgctacc
    10621 gttatgaacg gtttggaaga actccgtgtt aaggaaagcg accgtctttc tgctgtcgca
    10681 aacggtctca agctcaacgg tgttgattgc gatgaaggtg agacttctct cgtcgtgcgt
    10741 ggtcgtcctg acggtaaggg tctcggtaac gcttctggag cagctgtcgc tacccacctc
    10801 gatcaccgta tcgctatgag cttcctcgtt atgggtctcg tttctgaaaa ccctgttact
    10861 gttgatgatg ctactatgat cgctactagc ttcccagagt tcatggattt gatggctggt
    10921 cttggagcta agatcgaact ctccgacact aaggctgctt gagatcgttc aaacatttgg
    10981 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
    11041 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
    11101 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
    11161 tagcgcgcaa actaggataa gtcgacaatg ccatcgcctc gaaatctcag aagctccctt
    11221 gctttgctct tccaccaaag gccaccctta gatctcccaa gttttccatg atctccacca
    11281 ttccttctgg ctccaagtta gtgtctttct ttcttttctt tttggctggc gttttattca
    11341 ttattgtaat aacttgatca ttctcgccta taatcatgga tcataacctt ttcttcttct
    11401 tcttcttctt cttcttcttt tacttaaagc tgattttgaa tctgggtttt tgttgttttg
    11461 tctaactgac atggctttca ggacttctct tttatgatat ctcacaatct taacgttctt
    11521 gttgtttaat ggcctctaat cattgttatg atgagatttg aacttttaca aattatgata
    11581 ttgttgtctt tgaatccttg gtaataatga tgatgtatga gtatgagaat gaaagtaatc
    11641 ccaaagttgg tttattttta ctgaacacag agaggttggg aatctgaaaa agcctttcac
    11701 gcctccaaag gaggtgcctg ttcagatcac ccactccatg ccgcctcaca agattgagat
    11761 ctttaaatct ttggagggct gggctgagaa caacattctg actcacctca aaccagttga
    11821 gaaatgttgg caacccgccg actttcttcc agatcctaat tctgatggat ttcatgagca
    11881 agtcaaagag cttagggaaa gggcaaagga gatcccagat gattactttg tagttttggt
    11941 tggtgatatg atcaccgagg aagccctttc aacttatcaa acaatgctta ataccttgga
    12001 tggaactcgt gatgagacag gtgctagcaa gcttggcact ggccgtcgtt ttacaacgtc
    12061 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
    12121 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
    12181 tgaatggcga atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt
    12241 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
    12301 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
    12361 tg
//
