LOCUS       V004805                 3990 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004805
VERSION     V004805
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Uhlich GA, Chen CY.
  TITLE     A cloning vector for creation of Escherichia colilacZ translational
            fusions and generation of linear template for chromosomal
            integration
  JOURNAL   Plasmid 67 (3), 259-263 (2012)
   PUBMED   22197962
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Chen C-Y., Uhlich GA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2011) Molecular Characterization of Foodborne
            Pathogens RU, US Department of Agriculture, 600 E. Mermaid Lane,
            Wyndmoor, PA 19038, USA
REFERENCE   3  (bases 1 to 3990)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3990)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2012"; volume: "67"; issue: "3"; pages: "259-263"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (26-SEP-2011) Molecular Characterization of Foodborne Pathogens RU,
            US Department of Agriculture, 600 E. Mermaid Lane, Wyndmoor, PA
            19038, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..21
                     /label="LacIF primer binding site"
                     /note="LacIF primer binding site"
     primer_bind     51..76
                     /note="SpecF primer binding site; primer with additional
                     sequence at the 5' end 'AAGGAATT'"
     CDS             228..1007
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     primer_bind     complement(1257..1294)
                     /label="SpecR primer binding site"
                     /note="SpecR primer binding site"
     misc_feature    1279..1302
                     /label="multiple cloning site"
                     /note="multiple cloning site"
     CDS             1298..1408
                     /codon_start=1
                     /product="lacZ"
                     /label="lacZ"
                     /note="lacZ reading frame for translational fusion"
                     /protein_id="AFA52512.1"
                     /translation="DPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR"
     primer_bind     complement(1346..1367)
                     /label="LacZR primer binding site"
                     /note="LacZR primer binding site"
     primer_bind     complement(1389..1409)
                     /label="LacZR2 primer binding site"
                     /note="LacZR2 primer binding site"
     rep_origin      complement(1418..1806)
                     /direction=LEFT
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1836..2393)
                     /codon_start=1
                     /gene="traI"
                     /product="TraI"
                     /label="traI"
                     /note="TraI DNA relaxase"
                     /protein_id="AFA52510.1"
                     /translation="MIAKHVPMRSIKKSDFAELVKYITDEQGKTERLGHVRVTNCEANT
                     LPAVMAEVMATQHGNTRSEADKTYHLLVSFRAGEKPDAETLRAIEDRICAGLGFAEHQR
                     VSAVHHDTDNLHIHIAINKIHPTRNTIHEPYRAYRALADLCATLERDYGLERDNHETRQ
                     RVSENRANDMERHAGVESLVGWI"
     gene            complement(1836..2393)
                     /gene="traI"
                     /label="traI"
     CDS             complement(2431..2799)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(2832..2941)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             3167..3563
                     /codon_start=1
                     /gene="traK"
                     /product="TraK"
                     /label="traK"
                     /note="oriT binding protein"
                     /protein_id="AFA52508.1"
                     /translation="MPKSYTDELAEWVESRAAKKRRRDEAAVAFLAVRADVEAALASGY
                     ALVTIWEHMRETGKVKFSYETFRSHARRHIKAKPADVPAPQAKAAEPAPAPKTPEPRRP
                     KQGGKAEKPAPAAAPTGFTFNPTPDKKD"
     gene            3167..3563
                     /gene="traK"
                     /label="traK"
     promoter        3670..3772
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gggttgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga ctagaggatc
       61 tgtataataa agaataatta ttaatctgta gacaaattgt gaaaggatgt acttaaacgc
      121 taacggtcag ctttattgaa cagtaattta agtatatgtc caatctaggg taagtaaatt
      181 gagtatcaat ataaacttta tatgaacata atcaacgagg tgaaatcatg agcaatttga
      241 ttaacggaaa aataccaaat caagcgattc aaacattaaa aatcgtaaaa gatttatttg
      301 gaagttcaat agttggagta tatctatttg gttcagcagt aaatggtggt ttacgcatta
      361 acagcgatgt agatgttcta gtcgtcgtga atcatagttt acctcaatta actcgaaaaa
      421 aactaacaga aagactaatg actatatcag gaaagattgg aaatacggat tctgttagac
      481 cacttgaagt tacggttata aataggagtg aagttgtccc ttggcaatat cctccaaaaa
      541 gagaatttat atacggtgag tggctcaggg gtgaatttga gaatggacaa attcaggaac
      601 caagctatga tcctgatttg gctattgttt tagcacaagc aagaaagaat agtatttctc
      661 tatttggtcc tgattcttca agtatacttg tctccgtacc tttgacagat attcgaagag
      721 caattaagga ttctttgcca gaactaattg aggggataaa aggtgatgag cgtaatgtaa
      781 ttttaaccct agctcgaatg tggcaaacag tgactactgg tgaaattacc tcgaaagatg
      841 tcgctgcaga atgggctata cctcttttac ctaaagagca tgtaacttta ctggatatag
      901 ctagaaaagg ctatcgggga gagtgtgatg ataagtggga aggactatat tcaaaggtga
      961 aagcactcgt taagtatatg aaaaattcta tagaaacttc tctcaattag gctaatttta
     1021 ttgcaataac aggtgcttac ttttaaaact actgatttat tgataaatat tgaacaattt
     1081 ttgggaagaa taaagcgtcc tcttgtgaaa ttagagaacg gtttattact ttaatttagt
     1141 gaaacaattt gtaactattg aaaatagaaa gaaattgttc cttcgatagt ttattaatat
     1201 tagtgacatt tgcatgcttc aaagcctgtc ggaattggtt tttagtgaaa agaacacgat
     1261 attcacggtt tacccactgc ggccgcccgg gcttggggat cccgtcgttt tacaacgtcg
     1321 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     1381 cagctggcgt aatagcgaag aggcccgcaa attcccatgt cagccgttaa gtgttcctgt
     1441 gtcactcaaa attgctttga gaggctctaa gggcttctca gtgcgttaca tccctggctt
     1501 gttgtccaca accgttaaac cttaaaagct ttaaaagcct tatatattct tttttttctt
     1561 ataaaactta aaaccttaga ggctatttaa gttgctgatt tatattaatt ttattgttca
     1621 aacatgagag cttagtacgt gaaacatgag agcttagtac gttagccatg agagcttagt
     1681 acgttagcca tgagggttta gttcgttaaa catgagagct tagtacgtta aacatgagag
     1741 cttagtacgt gaaacatgag agcttagtac gtactatcaa caggttgaac tgctgatctt
     1801 cagatcctct acgccggacg catcgtggcc ggatcgatcc agccgaccag gctttccacg
     1861 cccgcgtgcc gctccatgtc gttcgcgcgg ttctcggaaa cgcgctgccg cgtttcgtga
     1921 ttgtcacgct caagcccgta gtcccgttcg agcgtcgcgc agaggtcagc gagggcgcgg
     1981 taggcccgat acggctcatg gatggtgttt cgggtcgggt gaatcttgtt gatggcgata
     2041 tggatgtgca ggttgtcggt gtcgtgatgc acggcactga cgcgctgatg ctcggcgaag
     2101 ccaagcccag cgcagatgcg gtcctcaatc gcgcgcaacg tctccgcgtc gggcttctct
     2161 cccgcgcgga agctaaccag caggtgatag gtcttgtcgg cctcggaacg ggtgttgccg
     2221 tgctgggtcg ccatcacctc ggccatgaca gcgggcaggg tgtttgcctc gcagttcgtg
     2281 acgcgcacgt gacccaggcg ctcggtcttg ccttgctcgt cggtgatgta cttcaccagc
     2341 tccgcgaagt cgctcttctt gatggagcgc atggggacgt gcttggcaat cacgcgcacc
     2401 ccccggccgt tttagcggct aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat
     2461 catgaccttg ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt
     2521 ggcatcaccg aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc
     2581 caggcggccc aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat agtccacgac
     2641 gcccgtgatt ttgtagccct ggccgacggc cagcaggtag gccgacaggc tcatgccggc
     2701 cgccgccgcc ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg
     2761 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     2821 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     2881 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     2941 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     3001 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     3061 ttatgcagcg gaaaagcgct gcttccctgc tgttttgtgg aatatctacc gactggaaac
     3121 aggcaaatgc aggaaattac tgaactgagg ggacaggcga gagacgatgc caaagagcta
     3181 caccgacgag ctggccgagt gggttgaatc ccgcgcggcc aagaagcgcc ggcgtgatga
     3241 ggctgcggtt gcgttcctgg cggtgagggc ggatgtcgag gcggcgttag cgtccggcta
     3301 tgcgctcgtc accatttggg agcacatgcg ggaaacgggg aaggtcaagt tctcctacga
     3361 gacgttccgc tcgcacgcca ggcggcacat caaggccaag cccgccgatg tgcccgcacc
     3421 gcaggccaag gctgcggaac ccgcgccggc acccaagacg ccggagccac ggcggccgaa
     3481 gcaggggggc aaggctgaaa agccggcccc cgctgcggcc ccgaccggct tcaccttcaa
     3541 cccaacaccg gacaaaaagg atcgggccct aatacctgtg acggaagatc acttcgcaga
     3601 ataaataaat cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa
     3661 atgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
     3721 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
     3781 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
     3841 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
     3901 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
     3961 tgcccgcctg atgaatgctc atccggaatt
//
