LOCUS       40924_29961        4227 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMCG16-S, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Krieg AM, Wu T, Weeratna R, Efler SM, Love-Homan L, Yang L, Yi AK, 
            Short D, Davis HL.
  TITLE     Sequence motifs in adenoviral DNA block immune activation by 
            stimulatory CpG motifs
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 95 (21), 12631-12636 (1998)
  PUBMED    9770537
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Wu T, Efler SM, Davis HL, Krieg AM, Schorr J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-1998) HGD, Loeb Health Research Institute, 725 
            Parkdale Ave., Ottawa, ON K1Y 4E9, Canada
REFERENCE   3  (bases 1 to 4227)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4227)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1998"; volume: "95"; issue: "21"; pages: 
            "12631-12636"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-MAR-1998) HGD, Loeb Health Research Institute, 725 Parkdale 
            Ave., Ottawa, ON K1Y 4E9, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    77..284
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(312..328)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(336..352)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    complement(407..428)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(716..1304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1450..2256)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2772..2788
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2795..2813
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2828..2844)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        2873..3252
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3253..3456
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             3528..4205
                     /codon_start=1
                     /label=HBsAg (S)
                     /note="major/small (S) envelope protein of hepatitis B
                     virus"
                     /translation="MENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGT
                     TVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGML
                     PVCPLIPGSSTTSTGPCRTCMTTAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLW
                     EWASARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCL
                     WVYI"
ORIGIN
        1 gaattcgagc tcccgggtac catggcatgc atcgatagat ctcgagtcta gactagagct
       61 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
      121 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
      181 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
      241 agcaaggggg aggattggga agacaatagc aggcatgctg gggaaggcct cggactagtg
      301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
      361 aacatacgag ccgcggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      421 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      481 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      541 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      601 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      661 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      721 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      781 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      841 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      901 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      961 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     1021 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     1081 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     1141 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     1201 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     1261 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     1321 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     1381 gctgtcgttg tgtcgtcaag tcagcgtaat gctctgccag tgttacaacc aattaaccaa
     1441 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     1501 atcaatacca tatttttgaa aaagtcgttt ctgtaatgaa ggagaaaact caccaaggca
     1561 gttccatagg atggcaagat cctggtatcg atctgcgatt ccaactcgtc caacatcaat
     1621 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     1681 gacgactgaa tctggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     1741 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccat tattcattcg
     1801 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     1861 aatcgaatgc aaacgtctca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     1921 aggatattct tctaatacct ggaatgctgt ttttccaggg atcgcagtgg tgagtaacca
     1981 tgcatcatca ggagtacgta taaaatgctt gatggtggga agaggcataa attctgtcag
     2041 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     2101 cagaaacaac tctggcgcat ctggcttccc atacaagcga tagattgtcg cacctgattg
     2161 ccctacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     2221 tcgtggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     2281 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
     2341 gagattttga gacacaacgt ggctttcccc ccccccccca tgacattaac ctataaaaat
     2401 aggcgtatca cgaggcccta ttttaaattc gaaagtactg gactccatga cgttcctgac
     2461 gtttccatga cgttcctgac gttggactcc atgacgttcc tgacgtttcc atgacgttcc
     2521 tgacgttggg actccatgac gttcctgacg tttccatgac gttcctgacg ttggactcca
     2581 tgacgttcct gacgtttcca tgacgttcct gacgttggac ctgttaacac gccattcgcc
     2641 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     2701 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     2761 gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt
     2821 ggggatcgat ccactagttc tagatccgat gtacgggcca gatatacgcg ttgacattga
     2881 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     2941 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     3001 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     3061 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     3121 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     3181 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     3241 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
     3301 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
     3361 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
     3421 aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag agaacccact
     3481 gcttactggc ttatcgaaat tgcggccgcc acggcgatat cctgaacatg gagaacatca
     3541 catcaggatt cctaggaccc cttctcgtgt tacaggcggg gtttttcttg ttgacaagaa
     3601 tcctcacaat accgcagagt ctagactcgt ggtggacttc tctcaatttt ctagggggaa
     3661 ctaccgtgtg tcttggccaa aattcgcagt ccccaacctc caatcactca ccaacctctt
     3721 gtcctccaac ttgtcctggt tatcgctgga tgtgtctgcg gcgttttatc atcttcctct
     3781 tcatcctgct gctatgcctc atcttcttgt tggttcttct ggactatcaa ggtatgttgc
     3841 ccgtttgtcc tctaattcca ggatcctcaa caaccagcac gggaccatgc cggacctgca
     3901 tgactactgc tcaaggaacc tctatgtatc cctcctgttg ctgtaccaaa ccttcggacg
     3961 gaaattgcac ctgtattccc atcccatcat cctgggcttt cggaaaattc ctatgggagt
     4021 gggcctcagc ccgtttctcc tggctcagtt tactagtgcc atttgttcag tggttcgtag
     4081 ggctttcccc cactgtttgg ctttcagtta tatggatgat gtggtattgg gggccaagtc
     4141 tgtacagcat cttgagtccc tttttaccgc tgttaccaat tttcttttgt ctttgggtat
     4201 acatttaaac cctactgcag aagcttc
//
