LOCUS       V004810                13012 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004810
VERSION     V004810
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Currie DH, Herring CD, Guss AM, Olson DG, Hogsett DA, Lynd LR.
  TITLE     Functional heterologous expression of an engineered full length CipA
            from Clostridium thermocellum in Thermoanaerobacterium
            saccharolyticum
  JOURNAL   Biotechnol Biofuels 6 (1), 32 (2013)
   PUBMED   23448319
REFERENCE   2  (bases 1 to 13012)
  AUTHORS   Currie DH, Herring CD, Guss AM, Olson DG, Hogsett DA, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2013) Thayer School of Engineering, Dartmouth
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 13012)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13012)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels"; date: "2013"; volume: "6"; issue: "1"; pages: "32"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-FEB-2013) Thayer School of Engineering, Dartmouth College, 14
            Engineering Drive, Hanover, NH 03755, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1500
                     /label="similar to xynA upstream region"
                     /note="similar to xynA upstream region"
     CDS             1501..3723
                     /gene="celS"
                     /label="Cellulose 1,4-beta-cellobiosidase (reducing end)
                     CelS"
                     /note="Cellulose 1,4-beta-cellobiosidase (reducing end)
                     CelS from Acetivibrio thermocellus (strain ATCC 27405 / DSM
                     1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 /
                     VPI 7372). Accession#: A3DH67"
     CDS             3739..3765
                     /label="9xHis"
                     /note="9xHis affinity tag"
     CDS             3966..4952
                     /codon_start=1
                     /product="pta"
                     /label="pta"
                     /protein_id="AGG53972.1"
                     /translation="MSIIQNIIEKAKSDKKKIVLPEGAEPRTLKAAEIVLKEGIADLVL
                     LGNEDEIRNAAKDLDISKAEIIDPVKSEMFDRYANDFYELRKNKGITLEKARETIKDNI
                     YFGCMMVKEGYADGLVSGAIHATADLLRPAFQIIKTAPGAKIVSSFFIMEVPNCEYGEN
                     GVFLFADCAVNPSPNAEELASIAVQSANTAKNLLGFEPKVAMLSFSTKGSASHELVDKV
                     RKATEIAKELMPDVAIDGELQLDAALVKEVAELKAPGSKVAGCANVLIFPDLQAGNIGY
                     KLVQRLAKANAIGPITQGMGAPVNDLSRGCSYRDIVDVIATTAVQAQ"
     CDS             4970..6181
                     /codon_start=1
                     /product="ack"
                     /label="ack"
                     /protein_id="AGG53973.1"
                     /translation="MKIMKILVINCGSSSLKYQLIESTDGNVLAKGLAERIGINDSMLT
                     HNANGEKIKIKKDMKDHKDAIKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGESFTSS
                     VLINDEVLKAITDCIELAPLHNPANIEGIKACQQIMPNVPMVAVFDTAFHQTMPDYAYL
                     YPIPYEYYTKYRIRRYGFHGTSHKYVSNRAAEILNKPIEDLKIITCHLGNGSSIAAVKY
                     GKSIDTSMGFTPLEGLAMGTRSGSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISG
                     ISSDFRDLEDAAFKNGDERAQLALNVFAYRVKKTIGAYAAAMGGVDVIVFTAGVGENGP
                     EIREFILDGLEFLGFSLDKEKNKVRGKETIISTPNSKVSVMVVPTNEEYMIAKDTEKIV
                     KSIK"
     CDS             6840..7631
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    7780..9217
                     /label="similar to xynA downstream region"
                     /note="similar to xynA downstream region"
     rep_origin      complement(9556..10101)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     promoter        10213..10241
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(10318..11118)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(11119..11339)
                     /label="URA3 promoter"
     misc_feature    11367..11870
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     oriT            complement(12269..12378)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     terminator      complement(12799..12826)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(12918..13004)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 atcttttctg gcctttaatg gcgctttcgg acatgtggaa agaagttgga tggaattcaa
       61 ttatttatct ggcggcaatg actgctatag acagtgagtt atatgaagcg gcaagtattg
      121 atggagcagg taggataaga agaattttct ccataacatt accatcaata gtgccaacca
      181 taaaaatcct tttaatatta aatgcaggat ggatattaaa tgctggattt gaacaagtat
      241 ttctattgtc aaatcccatg gtcatagact attcacagac attggaattg tatgtataca
      301 attatggtat accaatgggc agattttcat ttgcgacagc agccggtata tttaactcag
      361 ttgtttcttt agcaatggtt acattagcaa atagaatatc gaaagcactt agtggtgaaa
      421 gtgcatttta atctaattta caagatttca gggggttata aagtggctaa aaataataga
      481 ataaggttga gcacagaaga tattgtattt gacacgatta attacattct tctcacgatt
      541 gtaataatag tcaccctgta tccgtttgta catgtgttgg cagtttcatt taacgatgct
      601 atagatactg tgaggggtgg aatttatcta tttccaagaa aatttacgac ttttaattac
      661 gtttcaattt taagcgatcc tgagatttac aatgctacaa tcatttcagc attaagagct
      721 gttataggtt ctgttttaaa tgtgattgcc agcataatag ttgcatatgc cataagcaga
      781 agggattttg ttttaagagg tatagtttca aaagtattta cttactcaat gtattttaca
      841 agtggcttaa ttccatatta cttgctgatt agagatttac atcttatgaa caatttcttg
      901 gtttacatct tgccagggat agtaagcgca tggaatataa tggttgttag gagttatatt
      961 gatggcttgc cagccagtct tgtggaatcg gcaaaaatag atggcgctag tgagctgagg
     1021 ataatatttt ctatcatatt tccacttagt gttccagtat tagcgacaat aacgcttttt
     1081 gttgcagttg gacaatggaa ttcgtggttt gatacaatgc ttttttgttc catgaatcca
     1141 aatttaagca cattacagta tgaattgcaa aaagtattgc agtcgactca acagtttaca
     1201 caacaagcgt cttttgatat ggggttaaga aataattcta attcagttac cccggaaagc
     1261 ataagagctg ctatgacggt agtggcagtg gttcctatta tgatggttta tccattcttg
     1321 cagaagtatt ttgtaaaggg tttaaccatt ggcagtgtta agggttagta catctttggt
     1381 tttttataaa tgctgaaagg ggggaaattt aaaaatactt attagacaaa attttttaat
     1441 tgcgaggtga tagtaaataa atttttcaat aaattaaatt tactgaggga ggaagtaaga
     1501 atggtaaaaa gcagaaagat ttctattctg ttggcagttg caatgctggt atccataatg
     1561 atacccacaa ctgcattcgc aggtcctaca aaggcaccta caaaagatgg gacatcttat
     1621 aaggatcttt tccttgaact ctacggaaaa attaaagatc ctaagaacgg atatttcagc
     1681 ccagacgagg gaattcctta tcactcaatt gaaacattga tcgttgaagc gccggactac
     1741 ggtcacgtta ctaccagtga ggctttcagc tattatgtat ggcttgaagc aatgtatgga
     1801 aatctcacag gcaactggtc cggagtagaa acagcatgga aagttatgga ggattggata
     1861 attcctgaca gcacagagca gccgggtatg tcttcttaca atccaaacag ccctgccaca
     1921 tatgctgacg aatatgagga tccttcatac tatccttcag agttgaagtt tgataccgta
     1981 agagttggat ccgaccctgt acacaacgac cttgtatccg catacggtcc taacatgtac
     2041 ctcatgcact ggttgatgga cgttgacaac tggtacggtt ttggtacagg aacacgggca
     2101 acattcataa acaccttcca aagaggtgaa caggaatcca catgggaaac cattcctcat
     2161 ccgtcaatag aagagttcaa atacggcgga ccgaacggat tccttgattt gtttacaaag
     2221 gacagatcat atgcaaaaca gtggcgttat acaaacgctc ctgacgcaga aggccgtgct
     2281 atacaggctg tttactgggc aaacaaatgg gcaaaggagc agggtaaagg ttctgccgtt
     2341 gcttccgttg tatccaaggc tgcaaagatg ggtgacttct tgagaaacga catgttcgac
     2401 aaatacttca tgaagatcgg tgcacaggac aagactcctg ctaccggtta tgacagtgca
     2461 cactacctta tggcctggta tactgcatgg ggtggtggaa ttggtgcatc ctgggcatgg
     2521 aagatcggat gcagccacgc acacttcgga tatcagaacc cattccaggg atgggtaagt
     2581 gcaacacaga gcgactttgc tcctaaatca tccaacggta agagagactg gacaacaagc
     2641 tacaagagac agcttgaatt ctatcagtgg ttgcagtcgg ctgaaggtgg tattgccggt
     2701 ggagcaacca actcctggaa cggtagatat gagaaatatc ctgctggtac gtcaacgttc
     2761 tatggtatgg catatgttcc gcatcctgta tacgctgacc cgggtagtaa ccagtggttc
     2821 ggattccagg catggtcaat gcagcgtgta atggagtact acctcgaaac aggagattca
     2881 tcagttaaga atttgattaa gaagtgggtc gactgggtaa tgagcgaaat taagctctat
     2941 gacgatggaa catttgcaat tcctagcgac ctcgagtggt caggtcagcc tgatacatgg
     3001 accggaacat acacaggcaa cccgaacctc catgtaagag taacttctta cggtactgac
     3061 cttggtgttg caggttcact tgcaaatgct cttgcaactt atgccgcagc tacagaaaga
     3121 tgggaaggaa aacttgatac aaaagcaaga gacatggctg ctgaactggt taaccgtgca
     3181 tggtacaact tctactgctc tgaaggaaaa ggtgttgtta ctgaggaagc acgtgctgac
     3241 tacaaacgtt tctttgagca ggaagtatac gttccggcag gttggagcgg tactatgccg
     3301 aacggtgaca agattcagcc tggtattaag ttcatagaca tccgtacaaa atatagacaa
     3361 gatccttact acgatatagt atatcaggca tacttgagag gcgaagctcc tgtattgaat
     3421 tatcaccgct tctggcatga agttgacctt gcagttgcaa tgggtgtatt ggctacatac
     3481 ttcccggata tgacatataa agtacctggt actccttcta ctaaattata cggcgacgtc
     3541 aatgatgacg gaaaagttaa ctcaactgac gctgtagcat tgaagagata tgttttgaga
     3601 tcaggtataa gcatcaacac tgacaatgcc gatttgaatg aagacggcag agttaattca
     3661 actgacttag gaattttgaa gagatatatt ctcaaagaaa tagatacatt gccgtacaag
     3721 aacggaggag gaacaggaca tcatcatcat catcatcatc atcatcatta acgtgcccat
     3781 tgtgaagtgg attgtattct acaattaaac ctaatacgct cataatatgc gcctttctaa
     3841 aaaattatta attgtactta ttattttata aaaaatatgt taaaatgtaa aatgtgtata
     3901 caatatattt cttcttagta agaggaatgt ataaaaataa atattttaaa ggaagggacg
     3961 atcttatgag cattattcaa aacatcattg aaaaagctaa aagcgataaa aagaaaattg
     4021 ttctgccaga aggtgcagaa cccaggacat taaaagctgc tgaaatagtt ttaaaagaag
     4081 ggattgcaga tttagtgctt cttggaaatg aagatgagat aagaaatgct gcaaaagact
     4141 tggacatatc caaagctgaa atcattgacc ctgtaaagtc tgaaatgttt gataggtatg
     4201 ctaatgattt ctatgagtta aggaagaaca aaggaatcac gttggaaaaa gccagagaaa
     4261 caatcaagga taatatctat tttggatgta tgatggttaa agaaggttat gctgatggat
     4321 tggtatctgg cgctattcat gctactgcag atttattaag acctgcattt cagataatta
     4381 aaacggctcc aggagcaaag atagtatcaa gcttttttat aatggaagtg cctaattgtg
     4441 aatatggtga aaatggtgta ttcttgtttg ctgattgtgc ggtcaaccca tcgcctaatg
     4501 cagaagaact tgcttctatt gccgtacaat ctgctaatac tgcaaagaat ttgttgggct
     4561 ttgaaccaaa agttgccatg ctatcatttt ctacaaaagg tagtgcatca catgaattag
     4621 tagataaagt aagaaaagcg acagagatag caaaagaatt gatgccagat gttgctatcg
     4681 acggtgaatt gcaattggat gctgctcttg ttaaagaagt tgcagagcta aaagcgccgg
     4741 gaagcaaagt tgcgggatgt gcaaatgtgc ttatattccc tgatttacaa gctggtaata
     4801 taggatataa gcttgtacag aggttagcta aggcaaatgc aattggacct ataacacaag
     4861 gaatgggtgc accggttaat gatttatcaa gaggatgcag ctatagagat attgttgacg
     4921 taatagcaac aacagctgtg caggctcaat aaaatgtaaa gtatggagga tgaaaattat
     4981 gaaaatactg gttattaatt gcggaagttc ttcgctaaaa tatcaactga ttgaatcaac
     5041 tgatggaaat gtgttggcaa aaggccttgc tgaaagaatc ggcataaatg attccatgtt
     5101 gacacataat gctaacggag aaaaaatcaa gataaaaaaa gacatgaaag atcacaaaga
     5161 cgcaataaaa ttggttttag atgctttggt aaacagtgac tacggcgtta taaaagatat
     5221 gtctgagata gatgctgtag gacatagagt tgttcacgga ggagaatctt ttacatcatc
     5281 agttctcata aatgatgaag tgttaaaagc gataacagat tgcatagaat tagctccact
     5341 gcacaatcct gctaatatag aaggaattaa agcttgccag caaatcatgc caaacgttcc
     5401 aatggtggcg gtatttgata cagcctttca tcagacaatg cctgattatg catatcttta
     5461 tccaatacct tatgaatact acacaaagta caggattaga agatatggat ttcatggcac
     5521 atcgcataaa tatgtttcaa atagggctgc agagattttg aataaaccta ttgaagattt
     5581 gaaaatcata acttgtcatc ttggaaatgg ctccagcatt gctgctgtca aatatggtaa
     5641 atcaattgac acaagcatgg gatttacacc attagaaggt ttggctatgg gtacacgatc
     5701 tggaagcata gacccatcca tcatttcgta tcttatggaa aaagaaaata taagcgctga
     5761 agaagtagta aatatattaa ataaaaaatc tggtgtttac ggtatttcag gaataagcag
     5821 cgattttaga gacttagaag atgccgcctt taaaaatgga gatgaaagag ctcagttggc
     5881 tttaaatgtg tttgcatatc gagtaaagaa gacgattggc gcttatgcag cagctatggg
     5941 aggcgtcgat gtcattgtat ttacagcagg tgttggtgaa aatggtcctg agatacgaga
     6001 atttatactt gatggattag agtttttagg gttcagcttg gataaagaaa aaaataaagt
     6061 cagaggaaaa gaaactatta tatctacgcc gaattcaaaa gttagcgtga tggttgtgcc
     6121 tactaatgaa gaatacatga ttgctaaaga tactgaaaag attgtaaaga gtataaaata
     6181 gcattcttga caaatgttta ccccattagt ataattaatt ttggcaatta tattggggtg
     6241 agaaaatgaa aattgattta tcaaaaatta aaggacatag gggccgcagc atcgaagtca
     6301 actacgtaaa acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa
     6361 acgagaattg gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag
     6421 acgaagagga tgaagaggat gaggaggcag attgccttga atatattgac aatactgata
     6481 agataatata tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     6541 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     6601 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     6661 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttcaaaacaa ctttgaaaaa
     6721 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     6781 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     6841 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     6901 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     6961 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     7021 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     7081 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     7141 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     7201 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     7261 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     7321 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     7381 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     7441 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     7501 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     7561 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     7621 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttttagaca tctaatcttt
     7681 tctgaagtac atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc
     7741 tagatagggg tcccgagcgc ctacgaggaa tttgtatcga aaaaacaaat aatctttaag
     7801 taaaaaggca gagaggaata atcttccctc tgccttttta ctttgtgata atttagactg
     7861 ttatttctat tgatatgtcg tttttatcta aattgaaatg aaattttcta tcattaatga
     7921 ttaattcata ttctcctaaa aagcctgtaa attcgaattc acctgtattg cttgtgacaa
     7981 gacgtgtttg atgtgtccac cattcgtctt taataagctt ttttaattcg taatatgatg
     8041 gcttgcatga attgtcctct ctaagaaggc ctgcaggcgc accaagccat gcattttcat
     8101 cgcagaaatt ccaccatgtg attgattcta ccattggatg tgaaaagaga gtcttataat
     8161 gttttacgac ttccgttgct tgtcgttctt ccccatcagg tgttgaaggc caatcactta
     8221 tctggtagtc atttaggtct tctatttcag gtggcatcaa atgccctgat aataatgtgt
     8281 tttctgtaaa atgcaatgga atattgaaat gcgaaaatct ttcgagtact tctaaagttt
     8341 tttcaactcc ccaatatcct tgatgcatgt gtgattggat ccctatggca tcaataggga
     8401 ttccagcttc aaggcatcct tctatgagta tttcatatga aattgatgta ttaaagtcat
     8461 ttataaggag aactgcttca ggattagctt ttttagcttc attaaaaact tcttttacga
     8521 ggcgaatacg tcctaattct ttgcatatcc tggttattcc attgtcgtac ttattaaaaa
     8581 taggcattat gacaacttca tttattacat cccatatatt cactaatcct ttaaaatcac
     8641 ttgcttcacg ttttatgcgg gatagctgag cctttaatat gtcttcattg ctcatatcta
     8701 agagccaagg tgctgttaca gtatgccagc aaagtggatg gccttttaca aggcaacctt
     8761 ttgatacaag ccattctgaa gcctttttga gtctatttgt gtctggcttt cctttttcag
     8821 gctcgaacct gccccaataa aatggaatcg tagcatagtt aaataagtca aaaaatttct
     8881 caaaacggtc ttcaattttt tctttatcat tttctttcaa ttcaccattg gcaagaggaa
     8941 gagaatcaaa tgctccacaa ccaaataaaa atttatgctt cacctgagat acagcgactt
     9001 ctgcatcttt tataggtgaa ccatctttct taactaactt tattttggcg attcctttgc
     9061 ggtgttttaa tttatctgca gcatccgcat tcattataat ccctccaaca tatgtaattg
     9121 tttataaata aaatttactc tcacactttg aataagtcaa taaatcatac ttataagact
     9181 aacataatat taaggcgttt ttttgtcgca tttgacttct atcagctgtc cctcctgttc
     9241 agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag
     9301 tgtatactgg cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca
     9361 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt
     9421 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg
     9481 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc
     9541 cgcggcaaag ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg
     9601 ctcaaatcag tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9661 cggctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     9721 atggccgcgt ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca
     9781 agctggactg tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact
     9841 atcgtcttga gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta
     9901 attgatttag aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca
     9961 agttttggtg actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag
    10021 agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg
    10081 cgcagaccaa aacgatctca agaagatcat cttattaatc agataaaata tttctagatt
    10141 tcagtgcaat ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg
    10201 taattctcat gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt
    10261 aaattgctaa cgcagtcagg cacctataca tgcatttact tataatacag ttttttagtt
    10321 ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc
    10381 taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc
    10441 tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc
    10501 taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc
    10561 tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc
    10621 cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag
    10681 gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg
    10741 gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc
    10801 agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa
    10861 aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc
    10921 agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa
    10981 ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc
    11041 gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt
    11101 atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg
    11161 ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa
    11221 tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa
    11281 atttcaaaga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa
    11341 agctagctta tcgatgggtc cttttcatca cgtgctataa aaataattat aatttaaatt
    11401 ttttaatata aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata
    11461 gtttttgttt tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt
    11521 atcttgctct tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa
    11581 gaccacacac gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct
    11641 atttaatctg cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt
    11701 ttttaaacct ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta
    11761 ctttctaaaa tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt
    11821 attccatcat taaaagatac gaggcgcgtg taagttacag gcaagcgatc tctaagaaac
    11881 cattattatc atgacattaa cctataaaaa aggcctctcg agctagagtc gatcttcgcc
    11941 agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag
    12001 agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg
    12061 tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc
    12121 gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg
    12181 cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc
    12241 ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg
    12301 agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct
    12361 acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc
    12421 ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata
    12481 atgaccccga agcagggtta tgcagcggaa aagcgctgct tccctgctgt tttgtggaat
    12541 atctaccgac tggaaacagg caaatgcagg aaattactga actgagggga caggcgagag
    12601 acgatgccaa agagctacac cgacgagctg gccgagtggg ttgaatcccg cgcggccaag
    12661 aagcgccggc gtgatgaggc tgcggttgcg ttcctggcgg tgagggcgga tgtcgatatg
    12721 cgtaaggaga aaataccgca tcaggcgcat atttgaatgt atttagaaaa ataaacaaaa
    12781 agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc
    12841 ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc
    12901 aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata
    12961 aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct gg
//
