LOCUS       40924_29926        7153 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mycobacteria-E.coli shuttle probe vector pMC210, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Fan XY, Ma H, Guo J, Li ZM, Cheng ZH, Guo SQ, Zhao GP.
  TITLE     A novel differential expression system for gene modulation in 
            Mycobacteria
  JOURNAL   Plasmid 61 (1), 39-46 (2009)
  PUBMED    18835406
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Fan X-Y., Li Z-M., Ma H, Guo J, Zhao G-P.
  TITLE     Analysis of promoter elements of Mycobacterium tuberculosis furA 
            gene in vivo and their application for recombinant BCG to induce 
            enhanced Th1 type immune responses
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7153)
  AUTHORS   Fan X-Y., Ma H, Guo J, Li Z-M., Zhao G-P.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-APR-2008) Department of Microbiology, Fudan 
            University, Shanghai 200433, China
REFERENCE   4  (bases 1 to 7153)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7153)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2009"; volume: "61"; issue: "1"; pages: "39-46"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (28-APR-2008) Department of Microbiology, Fudan University, Shanghai
            200433, China"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             120..932
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1267..1855
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      2014..3909
                     /label=mycobacterial OriM region derived from pAL5000
                     /note="mycobacterial OriM region derived from pAL5000"
     primer_bind     3953..3969
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3971..7015
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     terminator      7071..7117
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 gctagcaaca aagcgacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
       61 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
      121 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
      181 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
      241 atcgcttgta tgggaagccc catgcgccag agttgtttct gaaacatggc aaaggtagcg
      301 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
      361 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
      421 tccccgggaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
      481 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
      541 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
      601 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
      661 aaatgcataa tcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
      721 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
      781 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
      841 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
      901 tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac
      961 actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa atcgaacttt
     1021 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa
     1081 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc
     1141 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc
     1201 ttcacgaggc agacctcact agttccactg agcgtcagac cccgtagaaa agatcaaagg
     1261 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1321 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1381 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     1441 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1501 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1561 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1621 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     1681 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1741 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1801 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1861 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     1921 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     1981 cgctcgccgc agccgaacga ccgagcgcaa cgcgtgcggc cgcacgcgtg agcccaccag
     2041 ctccgtaagt tcgggtgctg tgtggctcgt acccgcgcat tcaggcggca gggggtctaa
     2101 cgggtctaag gcggcgtgta cggccgccac agcggctctt agcggcccgg aaacgtcctc
     2161 gaaacgacgc atgtgttcct cctggttggt acaggtggtt gggggtgctc ggctgtcgct
     2221 ggtgtttcat catcagggct cgacgggaga gcgggggagt gtgcagttgt ggggtggccc
     2281 ctcagcgaaa tatctgactt ggagctcgtg tcggaccata caccggtgat taatcgtggt
     2341 ctactaccaa gcgtgagcca cgtcgccgac gaatttgagc agctctggct gccgtactgg
     2401 ccgctggcaa gcgacgatct gctcgagggg atctaccgcc aaagccgcgc gtcggcccta
     2461 ggccgccggt acatcgaggc gaacccaaca gcgctggcaa acctgctggt cgtggacgta
     2521 gaccatccag acgcagcgct ccgagcgctc agcgcccggg ggtcccatcc gctgcccaac
     2581 gcgatcgtgg gcaatcgcgc caacggccac gcacacgcag tgtgggcact caacgcccct
     2641 gttccacgca ccgaatacgc gcggcgtaag ccgctcgcat acatggcggc gtgcgccgaa
     2701 ggccttcggc gcgccgtcga tggcgaccgc agttactcag gcctcatgac caaaaacccc
     2761 ggccacatcg cctgggaaac ggaatggctc cactcagatc tctacacact cagccacatc
     2821 gaggccgagc tcggcgcgaa catgccaccg ccgcgctggc gtcagcagac cacgtacaaa
     2881 gcggctccga cgccgctagg gcggaattgc gcactgttcg attccgtcag gttgtgggcc
     2941 tatcgtcccg ccctcatgcg gatctacctg ccgacccgga acgtggacgg actcggccgc
     3001 gcgatctatg ccgagtgcca cgcgcgaaac gccgaatttc cgtgcaacga cgtgtgtccc
     3061 ggaccgctac cggacagcga ggtccgcgcc atcgccaaca gcatttggcg ttggatcaca
     3121 accaagtcgc gcatttgggc ggacgggatc gtggtctacg aggccacact cagtgcgcgc
     3181 cagtcggcca tctcgcggaa gggcgcagca gcgcgcacgg cggcgagcac agttgcgcgg
     3241 cgcgcaaagt ccgcgtcagc catgcatgga ggcattgcta tgagcgacgg ctacagcgac
     3301 ggctacagcg acggctacaa ccggcagccg actgtccgca aaaagcggcg cgtgaccgcc
     3361 gccgaaggcg ctcgaatcac cggactatcc gaacgccacg tcgtccggct cgtggcgcag
     3421 gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg aacgcatccg cgcctatcac
     3481 gacgacgagg gccactcttg gccgcaaacg gccaaacatt tcgggctgca tctggacacc
     3541 gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg cagaacagga agcggctcaa
     3601 aaggcccaca acgaagccga caatccaccg ctgttctaac gcaattgggg agcgggtgtc
     3661 gcgggggttc cgtggggggt tccgttgcaa cgggtcggac aggtaaaagt cctggtagac
     3721 gctagttttc tggtttgggc catgcctgtc tcgttgcgtg tttcgttgcg tccgttttga
     3781 ataccagcca gacgagacgg ggttctacga atcttggtcg ataccaagcc atttccgctg
     3841 aatatcgtgg agctcaccgc cagaatcggt ggttgtggtg atgtacgtgg cgaactccgt
     3901 tgtagtgctt gtggtggcat ccgtggcgcg gccgcggtac cagatcttta aatctagaac
     3961 tagtggatcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4021 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4081 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc
     4141 accagaagcg gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atactgtcgt
     4201 cgtcccctca aactggcaga tgcacggtta cgatgcgccc atctacacca acgtgaccta
     4261 tcccattacg gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct
     4321 cacatttaat gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg
     4381 cgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag
     4441 tcgtttgccg tctgaatttg acctgagcgc atttttacgc gccggagaaa accgcctcgc
     4501 ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa gatcaggata tgtggcggat
     4561 gagcggcatt ttccgtgacg tctcgttgct gcataaaccg actacacaaa tcagcgattt
     4621 ccatgttgcc actcgcttta atgatgattt cagccgcgct gtactggagg ctgaagttca
     4681 gatgtgcggc gagttgcgtg actacctacg ggtaacagtt tctttatggc agggtgaaac
     4741 gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta
     4801 tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat
     4861 cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc gacggcacgc tgattgaagc
     4921 agaagcctgc gatgtcggtt tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa
     4981 cggcaagccg ttgctgattc gaggcgttaa ccgtcacgag catcatcctc tgcatggtca
     5041 ggtcatggat gagcagacga tggtgcagga tatcctgctg atgaagcaga acaactttaa
     5101 cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg tacacgctgt gcgaccgcta
     5161 cggcctgtat gtggtggatg aagccaatat tgaaacccac ggcatggtgc caatgaatcg
     5221 tctgaccgat gatccgcgct ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca
     5281 gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg gggaatgaat caggccacgg
     5341 cgctaatcac gacgcgctgt atcgctggat caaatctgtc gatccttccc gcccggtgca
     5401 gtatgaaggc ggcggagccg acaccacggc caccgatatt atttgcccga tgtacgcgcg
     5461 cgtggatgaa gaccagccct tcccggctgt gccgaaatgg tccatcaaaa aatggctttc
     5521 gctacctgga gagacgcgcc cgctgatcct ttgcgaatac gcccacgcga tgggtaacag
     5581 tcttggcggt ttcgctaaat actggcaggc gtttcgtcag tatccccgtt tacagggcgg
     5641 cttcgtctgg gactgggtgg atcagtcgct gattaaatat gatgaaaacg gcaacccgtg
     5701 gtcggcttac ggcggtgatt ttggcgatac gccgaacgat cgccagttct gtatgaacgg
     5761 tctggtcttt gccgaccgca cgccgcatcc agcgctgacg gaagcaaaac accagcagca
     5821 gtttttccag ttccgtttat ccgggcaaac catcgaagtg accagcgaat acctgttccg
     5881 tcatagcgat aacgagctcc tgcactggat ggtggcgctg gatggtaagc cgctggcaag
     5941 cggtgaagtg cctctggatg tcgctccaca aggtaaacag ttgattgaac tgcctgaact
     6001 accgcagccg gagagcgccg ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc
     6061 gaccgcatgg tcagaagccg ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa
     6121 cctcagtgtg acgctccccg ccgcgtccca cgccatcccg catctgacca ccagcgaaat
     6181 ggatttttgc atcgagctgg gtaataagcg ttggcaattt aaccgccagt caggctttct
     6241 ttcacagatg tggattggcg ataaaaaaca actgctgacg ccgctgcgcg atcagttcac
     6301 ccgtgcaccg ctggataacg acattggcgt aagtgaagcg acccgcattg accctaacgc
     6361 ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg
     6421 cacggcagat acacttgctg atgcggtgct gattacgacc gctcacgcgt ggcagcatca
     6481 ggggaaaacc ttatttatca gccggaaaac ctaccggatt gatggtagtg gtcaaatggc
     6541 gattaccgtt gatgttgaag tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa
     6601 ctgccagctg gcgcaggtag cagagcgggt aaactggctc ggattagggc cgcaagaaaa
     6661 ctatcccgac cgccttactg ccgcctgttt tgaccgctgg gatctgccat tgtcagacat
     6721 gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa
     6781 ttatggccca caccagtggc gcggcgactt ccagttcaac atcagccgct acagtcaaca
     6841 gcaactgatg gaaaccagcc atcgccatct gctgcacgcg gaagaaggca catggctgaa
     6901 tatcgacggt ttccatatgg ggattggtgg cgacgactcc tggagcccgt cagtatcggc
     6961 ggaattccag ctgagcgccg gtcgctacca ttaccagttg gtctggtgtc aaaaataaaa
     7021 gcttatcgat gtcgacgtag ttaactagcg tacgatcgac tgccaggcat caaataaaac
     7081 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcc ggccatcatg
     7141 gccgcggtga tca
//
