LOCUS       40924_29921       10782 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMC200, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10782)
  AUTHORS   Currie DH, Herring CD, Guss AM, Olson DG, Hogsett DA, Lynd LR.
  TITLE     Functional heterologous expression of an engineered full length CipA
            from Clostridium thermocellum in Thermoanaerobacterium 
            saccharolyticum
  JOURNAL   Biotechnol Biofuels 6 (1), 32 (2013)
  PUBMED    23448319
REFERENCE   2  (bases 1 to 10782)
  AUTHORS   Currie DH, Herring CD, Guss AM, Olson DG, Hogsett DA, Lynd LR.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2013) Thayer School of Engineering, Dartmouth 
            College, 14 Engineering Drive, Hanover, NH 03755, USA
REFERENCE   3  (bases 1 to 10782)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10782)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Biofuels"; date: "2013"; volume: "6"; issue: "1"; pages: "32"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2013) Thayer School of Engineering, Dartmouth College, 14 
            Engineering Drive, Hanover, NH 03755, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..1500
                     /label=similar to xynA upstream region
                     /note="similar to xynA upstream region"
     misc_feature    1501..1541
                     /label=restriction cassette
                     /note="restriction cassette"
     CDS             1736..2722
                     /codon_start=1
                     /product="pta"
                     /label=pta
                     /protein_id="AGG53967.1"
                     /translation="MSIIQNIIEKAKSDKKKIVLPEGAEPRTLKAAEIVLKEGIADLVL
                     LGNEDEIRNAAKDLDISKAEIIDPVKSEMFDRYANDFYELRKNKGITLEKARETIKDNI
                     YFGCMMVKEGYADGLVSGAIHATADLLRPAFQIIKTAPGAKIVSSFFIMEVPNCEYGEN
                     GVFLFADCAVNPSPNAEELASIAVQSANTAKNLLGFEPKVAMLSFSTKGSASHELVDKV
                     RKATEIAKELMPDVAIDGELQLDAALVKEVAELKAPGSKVAGCANVLIFPDLQAGNIGY
                     KLVQRLAKANAIGPITQGMGAPVNDLSRGCSYRDIVDVIATTAVQAQ"
     CDS             2740..3951
                     /codon_start=1
                     /product="ack"
                     /label=ack
                     /protein_id="AGG53968.1"
                     /translation="MKIMKILVINCGSSSLKYQLIESTDGNVLAKGLAERIGINDSMLT
                     HNANGEKIKIKKDMKDHKDAIKLVLDALVNSDYGVIKDMSEIDAVGHRVVHGGESFTSS
                     VLINDEVLKAITDCIELAPLHNPANIEGIKACQQIMPNVPMVAVFDTAFHQTMPDYAYL
                     YPIPYEYYTKYRIRRYGFHGTSHKYVSNRAAEILNKPIEDLKIITCHLGNGSSIAAVKY
                     GKSIDTSMGFTPLEGLAMGTRSGSIDPSIISYLMEKENISAEEVVNILNKKSGVYGISG
                     ISSDFRDLEDAAFKNGDERAQLALNVFAYRVKKTIGAYAAAMGGVDVIVFTAGVGENGP
                     EIREFILDGLEFLGFSLDKEKNKVRGKETIISTPNSKVSVMVVPTNEEYMIAKDTEKIV
                     KSIK"
     CDS             4610..5401
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5550..6987
                     /label=similar to xynA downstream region
                     /note="similar to xynA downstream region"
     rep_origin      complement(7326..7871)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        7983..8011
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(8088..8888)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8889..9109)
                     /label=URA3 promoter
     misc_feature    9137..9640
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     oriT            complement(10039..10148)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     terminator      complement(10569..10596)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(10688..10774)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 atcttttctg gcctttaatg gcgctttcgg acatgtggaa agaagttgga tggaattcaa
       61 ttatttatct ggcggcaatg actgctatag acagtgagtt atatgaagcg gcaagtattg
      121 atggagcagg taggataaga agaattttct ccataacatt accatcaata gtgccaacca
      181 taaaaatcct tttaatatta aatgcaggat ggatattaaa tgctggattt gaacaagtat
      241 ttctattgtc aaatcccatg gtcatagact attcacagac attggaattg tatgtataca
      301 attatggtat accaatgggc agattttcat ttgcgacagc agccggtata tttaactcag
      361 ttgtttcttt agcaatggtt acattagcaa atagaatatc gaaagcactt agtggtgaaa
      421 gtgcatttta atctaattta caagatttca gggggttata aagtggctaa aaataataga
      481 ataaggttga gcacagaaga tattgtattt gacacgatta attacattct tctcacgatt
      541 gtaataatag tcaccctgta tccgtttgta catgtgttgg cagtttcatt taacgatgct
      601 atagatactg tgaggggtgg aatttatcta tttccaagaa aatttacgac ttttaattac
      661 gtttcaattt taagcgatcc tgagatttac aatgctacaa tcatttcagc attaagagct
      721 gttataggtt ctgttttaaa tgtgattgcc agcataatag ttgcatatgc cataagcaga
      781 agggattttg ttttaagagg tatagtttca aaagtattta cttactcaat gtattttaca
      841 agtggcttaa ttccatatta cttgctgatt agagatttac atcttatgaa caatttcttg
      901 gtttacatct tgccagggat agtaagcgca tggaatataa tggttgttag gagttatatt
      961 gatggcttgc cagccagtct tgtggaatcg gcaaaaatag atggcgctag tgagctgagg
     1021 ataatatttt ctatcatatt tccacttagt gttccagtat tagcgacaat aacgcttttt
     1081 gttgcagttg gacaatggaa ttcgtggttt gatacaatgc ttttttgttc catgaatcca
     1141 aatttaagca cattacagta tgaattgcaa aaagtattgc agtcgactca acagtttaca
     1201 caacaagcgt cttttgatat ggggttaaga aataattcta attcagttac cccggaaagc
     1261 ataagagctg ctatgacggt agtggcagtg gttcctatta tgatggttta tccattcttg
     1321 cagaagtatt ttgtaaaggg tttaaccatt ggcagtgtta agggttagta catctttggt
     1381 tttttataaa tgctgaaagg ggggaaattt aaaaatactt attagacaaa attttttaat
     1441 tgcgaggtga tagtaaataa atttttcaat aaattaaatt tactgaggga ggaagtaaga
     1501 gagctcgaat tctctagagg atccccggga agcttcagct gcgtgcccat tgtgaagtgg
     1561 attgtattct acaattaaac ctaatacgct cataatatgc gcctttctaa aaaattatta
     1621 attgtactta ttattttata aaaaatatgt taaaatgtaa aatgtgtata caatatattt
     1681 cttcttagta agaggaatgt ataaaaataa atattttaaa ggaagggacg atcttatgag
     1741 cattattcaa aacatcattg aaaaagctaa aagcgataaa aagaaaattg ttctgccaga
     1801 aggtgcagaa cccaggacat taaaagctgc tgaaatagtt ttaaaagaag ggattgcaga
     1861 tttagtgctt cttggaaatg aagatgagat aagaaatgct gcaaaagact tggacatatc
     1921 caaagctgaa atcattgacc ctgtaaagtc tgaaatgttt gataggtatg ctaatgattt
     1981 ctatgagtta aggaagaaca aaggaatcac gttggaaaaa gccagagaaa caatcaagga
     2041 taatatctat tttggatgta tgatggttaa agaaggttat gctgatggat tggtatctgg
     2101 cgctattcat gctactgcag atttattaag acctgcattt cagataatta aaacggctcc
     2161 aggagcaaag atagtatcaa gcttttttat aatggaagtg cctaattgtg aatatggtga
     2221 aaatggtgta ttcttgtttg ctgattgtgc ggtcaaccca tcgcctaatg cagaagaact
     2281 tgcttctatt gccgtacaat ctgctaatac tgcaaagaat ttgttgggct ttgaaccaaa
     2341 agttgccatg ctatcatttt ctacaaaagg tagtgcatca catgaattag tagataaagt
     2401 aagaaaagcg acagagatag caaaagaatt gatgccagat gttgctatcg acggtgaatt
     2461 gcaattggat gctgctcttg ttaaagaagt tgcagagcta aaagcgccgg gaagcaaagt
     2521 tgcgggatgt gcaaatgtgc ttatattccc tgatttacaa gctggtaata taggatataa
     2581 gcttgtacag aggttagcta aggcaaatgc aattggacct ataacacaag gaatgggtgc
     2641 accggttaat gatttatcaa gaggatgcag ctatagagat attgttgacg taatagcaac
     2701 aacagctgtg caggctcaat aaaatgtaaa gtatggagga tgaaaattat gaaaatactg
     2761 gttattaatt gcggaagttc ttcgctaaaa tatcaactga ttgaatcaac tgatggaaat
     2821 gtgttggcaa aaggccttgc tgaaagaatc ggcataaatg attccatgtt gacacataat
     2881 gctaacggag aaaaaatcaa gataaaaaaa gacatgaaag atcacaaaga cgcaataaaa
     2941 ttggttttag atgctttggt aaacagtgac tacggcgtta taaaagatat gtctgagata
     3001 gatgctgtag gacatagagt tgttcacgga ggagaatctt ttacatcatc agttctcata
     3061 aatgatgaag tgttaaaagc gataacagat tgcatagaat tagctccact gcacaatcct
     3121 gctaatatag aaggaattaa agcttgccag caaatcatgc caaacgttcc aatggtggcg
     3181 gtatttgata cagcctttca tcagacaatg cctgattatg catatcttta tccaatacct
     3241 tatgaatact acacaaagta caggattaga agatatggat ttcatggcac atcgcataaa
     3301 tatgtttcaa atagggctgc agagattttg aataaaccta ttgaagattt gaaaatcata
     3361 acttgtcatc ttggaaatgg ctccagcatt gctgctgtca aatatggtaa atcaattgac
     3421 acaagcatgg gatttacacc attagaaggt ttggctatgg gtacacgatc tggaagcata
     3481 gacccatcca tcatttcgta tcttatggaa aaagaaaata taagcgctga agaagtagta
     3541 aatatattaa ataaaaaatc tggtgtttac ggtatttcag gaataagcag cgattttaga
     3601 gacttagaag atgccgcctt taaaaatgga gatgaaagag ctcagttggc tttaaatgtg
     3661 tttgcatatc gagtaaagaa gacgattggc gcttatgcag cagctatggg aggcgtcgat
     3721 gtcattgtat ttacagcagg tgttggtgaa aatggtcctg agatacgaga atttatactt
     3781 gatggattag agtttttagg gttcagcttg gataaagaaa aaaataaagt cagaggaaaa
     3841 gaaactatta tatctacgcc gaattcaaaa gttagcgtga tggttgtgcc tactaatgaa
     3901 gaatacatga ttgctaaaga tactgaaaag attgtaaaga gtataaaata gcattcttga
     3961 caaatgttta ccccattagt ataattaatt ttggcaatta tattggggtg agaaaatgaa
     4021 aattgattta tcaaaaatta aaggacatag gggccgcagc atcgaagtca actacgtaaa
     4081 acccagcgaa ccatttgagg tgataggtaa gattataccg aggtatgaaa acgagaattg
     4141 gacctttaca gaattactct atgaagcgcc atatttaaaa agctaccaag acgaagagga
     4201 tgaagaggat gaggaggcag attgccttga atatattgac aatactgata agataatata
     4261 tcttttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc ataggcagcg
     4321 cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg
     4381 aaacccagga caataacctt atagcttgta aattctatca taattgtggt ttcaaaatcg
     4441 gctccgtcga tactatgtta tacgccaact ttcaaaacaa ctttgaaaaa gctgttttct
     4501 ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg ttataattag
     4561 cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa tggctaaaat
     4621 gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga
     4681 aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc tatatttaaa
     4741 aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa aggacatgat
     4801 gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg
     4861 ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag agtatgaaga
     4921 tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc tctttcactc
     4981 catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag ccgaattgga
     5041 ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag aagacactcc
     5101 atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg aagaggaact
     5161 tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg gcaaagtaag
     5221 tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca ttgccttctg
     5281 cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat tttttgactt
     5341 actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg atgaattgtt
     5401 ttagtaccta gatttagatg tctaaaaagc tttttagaca tctaatcttt tctgaagtac
     5461 atccgcaact gtccatactc tgatgtttta tatcttttct aaaagttcgc tagatagggg
     5521 tcccgagcgc ctacgaggaa tttgtatcga aaaaacaaat aatctttaag taaaaaggca
     5581 gagaggaata atcttccctc tgccttttta ctttgtgata atttagactg ttatttctat
     5641 tgatatgtcg tttttatcta aattgaaatg aaattttcta tcattaatga ttaattcata
     5701 ttctcctaaa aagcctgtaa attcgaattc acctgtattg cttgtgacaa gacgtgtttg
     5761 atgtgtccac cattcgtctt taataagctt ttttaattcg taatatgatg gcttgcatga
     5821 attgtcctct ctaagaaggc ctgcaggcgc accaagccat gcattttcat cgcagaaatt
     5881 ccaccatgtg attgattcta ccattggatg tgaaaagaga gtcttataat gttttacgac
     5941 ttccgttgct tgtcgttctt ccccatcagg tgttgaaggc caatcactta tctggtagtc
     6001 atttaggtct tctatttcag gtggcatcaa atgccctgat aataatgtgt tttctgtaaa
     6061 atgcaatgga atattgaaat gcgaaaatct ttcgagtact tctaaagttt tttcaactcc
     6121 ccaatatcct tgatgcatgt gtgattggat ccctatggca tcaataggga ttccagcttc
     6181 aaggcatcct tctatgagta tttcatatga aattgatgta ttaaagtcat ttataaggag
     6241 aactgcttca ggattagctt ttttagcttc attaaaaact tcttttacga ggcgaatacg
     6301 tcctaattct ttgcatatcc tggttattcc attgtcgtac ttattaaaaa taggcattat
     6361 gacaacttca tttattacat cccatatatt cactaatcct ttaaaatcac ttgcttcacg
     6421 ttttatgcgg gatagctgag cctttaatat gtcttcattg ctcatatcta agagccaagg
     6481 tgctgttaca gtatgccagc aaagtggatg gccttttaca aggcaacctt ttgatacaag
     6541 ccattctgaa gcctttttga gtctatttgt gtctggcttt cctttttcag gctcgaacct
     6601 gccccaataa aatggaatcg tagcatagtt aaataagtca aaaaatttct caaaacggtc
     6661 ttcaattttt tctttatcat tttctttcaa ttcaccattg gcaagaggaa gagaatcaaa
     6721 tgctccacaa ccaaataaaa atttatgctt cacctgagat acagcgactt ctgcatcttt
     6781 tataggtgaa ccatctttct taactaactt tattttggcg attcctttgc ggtgttttaa
     6841 tttatctgca gcatccgcat tcattataat ccctccaaca tatgtaattg tttataaata
     6901 aaatttactc tcacactttg aataagtcaa taaatcatac ttataagact aacataatat
     6961 taaggcgttt ttttgtcgca tttgacttct atcagctgtc cctcctgttc agctactgac
     7021 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag tgtatactgg
     7081 cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa
     7141 ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac
     7201 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg
     7261 agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag
     7321 ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag
     7381 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc
     7441 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     7501 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     7561 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
     7621 gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag
     7681 aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg
     7741 actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg
     7801 aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa
     7861 aacgatctca agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat
     7921 ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg taattctcat
     7981 gtttgacagc ttatcatcga taagctttaa tgcggtagtt tatcacagtt aaattgctaa
     8041 cgcagtcagg cacctataca tgcatttact tataatacag ttttttagtt ttgctggccg
     8101 catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca
     8161 tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc
     8221 acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca
     8281 ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca
     8341 ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat
     8401 tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt
     8461 tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca
     8521 ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc
     8581 aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac
     8641 ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata
     8701 tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat
     8761 tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata
     8821 ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct
     8881 ttcgacatga tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat
     8941 actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg
     9001 tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaaaga
     9061 aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaattgaaa agctagctta
     9121 tcgatgggtc cttttcatca cgtgctataa aaataattat aatttaaatt ttttaatata
     9181 aatatataaa ttaaaaatag aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt
     9241 tccgaagatg taaaagactc tagggggatc gccaacaaat actacctttt atcttgctct
     9301 tcctgctctc aggtattaat gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac
     9361 gaaaatcctg tgattttaca ttttacttat cgttaatcga atgtatatct atttaatctg
     9421 cttttcttgt ctaataaata tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct
     9481 ttgtttattt ttttttcttc attccgtaac tcttctacct tctttattta ctttctaaaa
     9541 tccaaataca aaacataaaa ataaataaac acagagtaaa ttcccaaatt attccatcat
     9601 taaaagatac gaggcgcgtg taagttacag gcaagcgatc tctaagaaac cattattatc
     9661 atgacattaa cctataaaaa aggcctctcg agctagagtc gatcttcgcc agcagggcga
     9721 ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca
     9781 ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt
     9841 ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca
     9901 tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct
     9961 tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat
    10021 ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca
    10081 ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta
    10141 tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa
    10201 atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga
    10261 agcagggtta tgcagcggaa aagcgctgct tccctgctgt tttgtggaat atctaccgac
    10321 tggaaacagg caaatgcagg aaattactga actgagggga caggcgagag acgatgccaa
    10381 agagctacac cgacgagctg gccgagtggg ttgaatcccg cgcggccaag aagcgccggc
    10441 gtgatgaggc tgcggttgcg ttcctggcgg tgagggcgga tgtcgatatg cgtaaggaga
    10501 aaataccgca tcaggcgcat atttgaatgt atttagaaaa ataaacaaaa agagtttgta
    10561 gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt
    10621 atggcgggcg tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc
    10681 ccggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
    10741 cccagtcttt cgactgagcc tttcgtttta tttgatgcct gg
//
