LOCUS       40924_29896        3967 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss gene targeting vector pMBL6attR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Kamisugi Y, Cuming AC, Cove DJ.
  TITLE     Parameters determining the efficiency of gene targeting in the moss 
            Physcomitrella patens
  JOURNAL   Nucleic Acids Res. 33 (19), E173 (2005)
  PUBMED    16282584
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Kamisugi Y, Cove DJ, Cuming AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2005) Centre for Plant Sciences, Leeds University,
            Leeds, West Yorkshire LS2 9JT, UK
REFERENCE   3  (bases 1 to 3967)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3967)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2005"; volume: "33"; issue: "19"; pages: "E173"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-OCT-2005) Centre for Plant Sciences, Leeds University, Leeds, 
            West Yorkshire LS2 9JT, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             394..696
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(740..864)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(923..939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(947..963)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(971..1001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1016..1037)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1325..1913)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2087..2944)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2945..3049)
                     /label=AmpR promoter
     protein_bind    3127..3251
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        3276..3306
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             join(3360..3967,1..49)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg
       61 atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat
      121 aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag
      181 cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca
      241 atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac
      301 gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg
      361 gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa
      421 agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg
      481 cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa
      541 ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc
      601 agtgtgccgg tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac
      661 atcaaaaacg ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac
      721 agccagtctg caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt
      781 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
      841 ttcagctttc ttgtacaaag tggttgatct agagcggccg ccaccgcggt ggtatctcga
      901 gatctagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      961 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1021 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     1081 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     1141 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     1201 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     1261 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     1321 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     1381 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     1441 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     1501 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     1561 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     1621 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     1681 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     1741 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     1801 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     1861 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     1921 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1981 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     2041 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     2101 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2161 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2221 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2281 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2341 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2401 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2461 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2521 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2581 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2641 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2701 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2761 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2821 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2881 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2941 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     3001 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3061 gaaaagtgcc acctgccagt gccaagttaa ttcgagctcc accgcggtgg cggccgctct
     3121 agatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
     3181 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
     3241 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
     3301 ataatgtgtg gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa
     3361 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     3421 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     3481 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     3541 acattcttgc ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg
     3601 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     3661 cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt
     3721 cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga
     3781 atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg
     3841 ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg
     3901 acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg
     3961 tcggcag
//
