LOCUS       40924_29891        4295 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector pMBL6, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Knight CD, Cove DJ, Cuming AC, Quatrano RS.
  TITLE     Moss Gene Technology
  JOURNAL   (in) Gilmartin,P.M. and Bowler,C. (Eds.); MOLECULAR PLANT BIOLOGY: 
            285-301; Oxford University Press, Oxford, NY, USA (2002)
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Machuka J, Knight CD, Cove DJ, Cuming AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2005) Centre for Plant Sciences, Leeds University,
            Leeds, West Yorkshire LS2 9JT, UK
REFERENCE   3  (bases 1 to 4295)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4295)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Gilmartin,P.M. and Bowler,C. (Eds.)"; volume: " MOLECULAR PLANT 
            BIOLOGY"; pages: " 285-301; Oxford University Press, Oxford, NY, USA
            (2002"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2005) Centre for Plant Sciences, Leeds University, Leeds, 
            West Yorkshire LS2 9JT, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4295
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    347..523
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     960..976
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1010..1026)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1076..1092)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1100..1116)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1124..1154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1169..1190)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1478..2066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2240..3097)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3098..3202)
                     /label=AmpR promoter
     misc_feature    3247..3354
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        3404..3749
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(3779..4295,1..275)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
       61 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
      121 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
      181 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
      241 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcggacg
      301 gtacgctgaa atcaccagtc tcttctacaa atctatctct ctctattttc tccataaata
      361 atgtgtgagt agtttcccga taagggaaat tagggttctt atagggtttc gctcatgtgt
      421 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
      481 tttctaattc ctaaaaccaa aatccagtac taaaatccag atctcctaaa gtccctatag
      541 atctttgtcg tgaatataaa ccagacacga gacgactaaa cctggagccc agacgccgtt
      601 cgaagctaga agtaccgctt aggcaggagg ccgttaggga aaagatgcta aggcagggtt
      661 ggttacgttg actcccccgt aggtttggtt taaatatgat gaagtggacg gaaggaagga
      721 ggaagacaag gaaggataag gttgcaggcc ctgtgcaagg taagaagatg gaaatttgat
      781 agaggtacgc tactatactt atactatacg ctaagggaat gcttgtattt ataccctata
      841 ccccctaata accccttatc aatttaagaa ataatccgca taagcccccg cttaaaaatt
      901 ggtatcagag ccatgaatag gtctatgacc aaaactcaag aggataacgg gccccccctc
      961 gaggtcgacg gtatcgataa gcttgatatc gaattcctgc agcccggggg atccactagt
     1021 tctagagcgg ccgccaccgc ggtggtatct cgagatctag cttggcgtaa tcatggtcat
     1081 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1141 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1201 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1261 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1321 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1381 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     1921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2341 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2521 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2701 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2821 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3061 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3181 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgcc agtgccaagc
     3241 taattcgagc tccaccgcgg tggcggccgc tctagaacta gtggatcccc cgggctgcag
     3301 gaattcgata tcaagcttat cgataccgtc gacctcgagg gggggcccgg tacccctact
     3361 ccaaaaatgt caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
     3421 gggtaatttc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcgaaa
     3481 ggacagtaga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcta
     3541 tcattcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc ncgaggagca
     3601 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgacatct
     3661 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
     3721 aaggaagttc atttcatttg gagaggacag cccaagcatg gagaaccgac ctgcaggcat
     3781 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3841 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3901 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactcca
     3961 agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     4021 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     4081 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     4141 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     4201 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     4261 gcatcagggg ctcgcgccag ccgaactgtt cgcca
//
