LOCUS       V015678                 5594 bp    DNA     circular SYN 12-JUN-2021
DEFINITION  Exported.
ACCESSION   V015678
VERSION     V015678
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Robertson WE, Funke LFH., de la Torre D, Fredens J, Elliott TS,
            Spinck M, Christova Y, Cervettini D, Boge FL, Liu KC, Buse S, Maslen
            S, Salmond GPC., Chin JW.
  TITLE     Sense Codon Reassignment Enables Viral Resistance and Encoded
            Polymer Synthesis
  JOURNAL   Science (2021) In press
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Robertson WE, Funke LFH., de la Torre D, Fredens J, Elliott TS,
            Spinck M, Christova Y, Cervettini D, Boge FL, Liu KC, Buse S, Maslen
            S, Salmond GPC., Chin JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-APR-2021) Protein and Nucleic Acid Chemistry, MRC
            Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge,
            Cambridgeshire CB2 0QH, United Kingdom
REFERENCE   3  (bases 1 to 5594)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Science
            (2021) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-APR-2021) Protein and Nucleic Acid Chemistry, MRC Laboratory of
            Molecular Biology, Francis Crick Avenue, Cambridge, Cambridgeshire
            CB2 0QH, United Kingdom"
FEATURES             Location/Qualifiers
     source          1..5594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             197..241
                     /label="S-Tag"
                     /note="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
     terminator      293..340
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(514..1326)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(1327..1418)
                     /label="AmpR promoter"
     rep_origin      complement(1482..2071)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     gene            2155..2180
                     /gene="glnS"
                     /label="glnS"
     regulatory      2155..2180
                     /gene="glnS"
                     /label="GlnS promoter"
                     /note="GlnS promoter"
                     /regulatory_class="promoter"
     CDS             2225..3586
                     /gene="pylS"
                     /label="Pyrrolysine--tRNA ligase"
                     /note="Pyrrolysine--tRNA ligase from Methanosarcina mazei
                     (strain ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11833 /
                     OCM 88). Accession#: Q8PWY1"
     CDS             3639..4607
                     /gene="tyrS"
                     /label="Tyrosine--tRNA ligase"
                     /note="Tyrosine--tRNA ligase from Archaeoglobus fulgidus
                     (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 /
                     VC-16). Accession#: O29482"
     regulatory      5143..5193
                     /label="lpp promoter"
                     /note="lpp promoter"
                     /regulatory_class="promoter"
     tRNA            5200..5271
                     /product="tRNA-Ser"
                     /label="pylToptserU(CGA)"
                     /note="pylToptserU(CGA)"
     tRNA            5311..5385
                     /product="tRNA-Tyr"
                     /label="Af tRNA TyrA01(CUA)"
                     /note="Af tRNA TyrA01(CUA)"
     gene            5392..5420
                     /gene="rrnC"
                     /label="rrnC"
     regulatory      5392..5420
                     /gene="rrnC"
                     /label="rrnC term"
                     /note="rrnC term"
                     /regulatory_class="terminator"
     primer_bind     complement(5430..5446)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gaatggtgca tgcaaggagc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc
       61 gatggatatg ttctggcggc cgcataatgc ttaagtcgaa cagaaagtaa tcgtattgta
      121 cacggccgca taatcgaaat taaattggat atcggcaggc cacgcgatcg ctgacgtcgg
      181 taccctcgag tctggtaaag aaaccgctgc tgcgaaattt gaacgccagc acatggactc
      241 gtctactagc gcagcttaat taacctaggc tgctgccacc gctgagcaat aactagcata
      301 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaacctc aggcatttga
      361 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata
      421 agcggctatt taacgaccct gccctgaacc gacgaccggg tcatcgtggc cggatcttgc
      481 ggcccctcgg cttgaacgaa ttgttagaca ttagaaaaac tcatcgagca tcaaatgaaa
      541 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
      601 actaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
      661 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
      721 atcaagactg aaatcaccat gagtgacgac actatccgga ctgaatggca aaagtttatg
      781 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
      841 atcaaccaaa ccgttattca ttcgactttg cgcctgagcg agacgaaata cgcggtcgct
      901 gttaaaagga caattacaaa caggaatgct atgcaaccgg cgcaggaaca ctgccagcgc
      961 atcaacaata ttttcaccac tatcaggata ttcttctaat acctggaatg ctgttttccc
     1021 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     1081 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     1141 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     1201 tcgatagatt gtcgcaccac tttgcccgac attatcgcga gcccatttat acccatataa
     1261 atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc gttgaatatg
     1321 gctcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1381 cggatacata tttgaatgta tttagaaaaa taaacaaata gctagcgagc aaaaggccag
     1441 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1501 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1561 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1621 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     1681 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     1741 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     1801 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     1861 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     1921 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     1981 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gttttttttg tttgcaagca
     2041 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2101 tgacgctcag tggaacgaaa actcacgtta agggattttg gggatcctcg ggagttgtca
     2161 gcctgtcccg cttataagat catacgccgt tatacgttgt ttacgctttg aggaatccca
     2221 tatgatggac aaaaaaccgc tgaacaccct gatctctgct accggtctgt ggatgtctcg
     2281 taccggtacc atccacaaaa tcaaacacca cgaagtttct cgttctaaaa tctacatcga
     2341 aatggcttgc ggtgaccacc tggttgttaa caactctcgt tcttctcgta ccgctcgtgc
     2401 tctgcgtcac cacaaatacc gtaaaacctg caaacgttgc cgtgtttctg acgaagacct
     2461 gaacaaattc ctgaccaaag ctaacgaaga ccagacctct gttaaagtta aagttgtttc
     2521 tgctccgacc cgtaccaaaa aagctatgcc gaaatctgtt gctcgtgctc cgaaaccgct
     2581 ggaaaacacc gaagctgctc aggctcagcc gtctggttct aaattctctc cggctatccc
     2641 ggtttctacc caggaatctg tttctgttcc ggcttctgtt tctacctcta tctcttctat
     2701 ctctaccggt gctaccgctt ctgctctggt taaaggtaac accaacccga tcacctctat
     2761 gtctgctccg gttcaggctt ctgctccggc tctgaccaaa tctcagaccg accgtctgga
     2821 agttctgctg aacccgaaag acgaaatctc tctgaactct ggtaaaccgt tccgtgaact
     2881 ggaatctgaa ctgctgtctc gtcgtaaaaa agacttacaa cagatctacg ctgaagaacg
     2941 tgaaaactac ctgggtaaac tggaacgtga aatcacgcgt ttcttcgttg accgtggttt
     3001 cctggaaatc aaatctccga tcctgatccc gctggaatac atcgaacgta tgggtatcga
     3061 caacgacacc gaactgtcta aacagatctt ccgtgttgac aaaaacttct gcctgcgtcc
     3121 gatgctggct ccgaacctgt acaactacct gcgtaaactg gaccgtgctc tgccggaccc
     3181 gatcaaaatc ttcgaaatcg gtccgtgcta ccgtaaagaa tctgacggta aagaacacct
     3241 ggaagaattc accatgctga acttctgcca gatgggttct ggttgcaccc gtgaaaacct
     3301 ggaatctatc atcaccgact tcctgaacca cctgggtatc gacttcaaaa tcgttggtga
     3361 ctcttgcatg gtttacggtg acaccctgga cgttatgcac ggtgacctgg aactgtcttc
     3421 tgctgttgtt ggtccgatcc cgctggaccg tgaatggggt atcgacaaac cgtggatcgg
     3481 tgctggtttc ggtctggaac gtctgctgaa agttaaacac gacttcaaaa acatcaaacg
     3541 tgctgctcgt tctgaatctt actacaacgg tatctctacc aacctgtaac ctattgaagg
     3601 gttgctaaga ataacaagat caaataagga ggttttttat ggacattacg gaaaaactgc
     3661 gcttgattac gcgcaacgcg gaggaagtcg taacagaaga agagttgcgt cagttgattg
     3721 aaacaaaaga aaaaccccgt gcaattgtag gctatgaacc aagtggcgag atccatttag
     3781 gtcatatgat gaccgtccag aaactgatgg atttacaaga ggcgggattt gagattattg
     3841 tcatgcttgc ggacatcctg gcatacttga atgaaaaagg tacattcgag gagattgccg
     3901 aagtggcaga ctacaataaa aaagtcttca ttgcattagg acttgacgaa agccgcgcca
     3961 aattcgtctt gggtagcgaa tatgagctga gccgtgacta cgtgttagac gtgttaaaga
     4021 tggcccgtat cactacgctt aatcgtgcgc gtcgcagcat ggatgaggtc agccgccgca
     4081 aggaagatcc aatggtttcc caaatgattt acccgcttat gcaggcactg acgggggctc
     4141 atttaggagt ggaccttgcc gtaggtggaa tcgaccaacg taagatccat atgctggcgc
     4201 gcgaaaacct gcctcgcttg ggatatagta gcccggtgtg tttacatacg ccgattctgg
     4261 tggggttaga cggacaaaag atgagtagca gtaagggaaa ttacattagt gttcgtgatc
     4321 ctccagaaga ggtggagcgt aaaatccgta aagcttattg cccggcagga gtggtcgagg
     4381 agaatccgat tctggatatt gccaagtatc acattttgcc ccgttttggt aagatcgtag
     4441 tagagcgcga tgctaaggtg ggaggggacg ttgaatacgc gagtttcgag gaattggcag
     4501 aagactttaa gtctggtcag ctgcatccag ggaggttaaa gatcgcggtc gctaaatatt
     4561 tgaatatgct gttggaggat gcccgtaaac gtctgggcgt gtctgtttaa ggaggacatc
     4621 ctgccgtttc aaacgctaaa ttgcctgatg cgctacgctt atcaggccta catgatctct
     4681 gcaatatatt gagtttgcgt gcttttgtag gccggataag gcgttcacgc cgcatccggc
     4741 aagaaacagc aaacaatcca aaacgccgcg ttcggcggtc gacacagatg taggtgttcc
     4801 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg
     4861 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac
     4921 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     4981 ccgcgcccac cggaaggagc taccggcagc ggtgcggact gttgtaactc agaataagaa
     5041 atgaggccgc tcatggcgtt ctgttgcccg tctcactggt gaaaagaaaa acaaccctgg
     5101 cgccgcttct ttgagcgaac gatcaaaaat aagtggcgcc ccatcaaaaa aatattctca
     5161 acataaaaaa ctttgtgtaa tacttgtaac gctagatctg gaaacgtgat catgtagatc
     5221 gaacggtctc gaaaaccgtt cagtggggtt agattcccca cgtttccgcc aaattttgca
     5281 cccagcaaac ttggtacgta aacgcatcgt cccgccctag ctcagaggta gagcgtgctt
     5341 ctctaaaaag catggtcccc ggttcaaatc ctgggggcgg gaccaactag tatccttagc
     5401 gaaagctaag gatttttttt aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     5461 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagacgc
     5521 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     5581 accgccgccg caag
//
