LOCUS       V004824                 6592 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004824
VERSION     V004824
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6592)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6592)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(625..1500)
                     /codon_start=1
                     /note="unnamed protein product; Salmonella hisG"
                     /protein_id="SJL88114.1"
                     /translation="MLDNTRLRIAIQKSGRLSDDSRELLARCGIKINLHTQRLIAMAEN
                     MPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYLTLRRLDFGGC
                     RLSLATPVDEAWDGPAALDGKRIATSYPHLLKRYLDQKGVSFKSCLLNGSVEVAPRAGL
                     ADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMAQSKQELIDKLLTRIQGVIQ
                     ARESKYIMMHAPSERLEEVIALLPGAERPTILPLAGEQQRVAMHMVSSETLFWETMDPR
                     DNRVIDPWRN"
     CDS             complement(2092..2901)
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     CDS             complement(3370..4227)
                     /codon_start=1
                     /note="unnamed protein product; Salmonella hisG"
                     /protein_id="SJL88116.1"
                     /translation="MLDNTRLRIAIQKSGRLSDDSRELLARCGIKINLHTQRLIAMAEN
                     MPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYLTLRRLDFGGC
                     RLSLATPVDEAWDGPAALDGKRIATSYPHLLKRYLDQKGVSFKSCLLNGSVEVAPRAGL
                     ADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMAQSKQELIDKLLTRIQGVIQ
                     ARESKYIMMHAPSERLEEVIALLPGAERPTILPLAGEQQRVAMHMVSSETLFWETMDPL
                     PCYP"
     primer_bind     complement(4371..4387)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4395..4411)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4419..4449)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4464..4485)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4773..5361)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5535..6392)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6393..6497)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccctgttc cagtcaatca gggtattgaa gctcatggtc tttactccat
      481 cacagggttc cgccttatcc ggcctacaga acccaaaata tcaacgcatt acgtaggcct
      541 gataagcgca gcgccatcag gcgtcagatc actccatcat cttctcgatc ggcagtacca
      601 gaatcgagct ggcgccaagc gctttcagtt tctccatgga tctattaccc tgttatccct
      661 aggatccatg gtttcccaga acaacgtttc gctgctgacc atgtgcatcg ccacgcgctg
      721 ttgctcgcct gccagcggca gaattgtcgg cctttcggcg cctggcagca gggcgataac
      781 ctcttccagg cgttcacttg gcgcgtgcat catgatgtat ttcgattcgc gcgcctgaat
      841 cacgccctga atacgggtca gcaatttatc gatcagctct tgcttgctct gtgccatctc
      901 accgtcgcgc tgaatcagac aggctttaga gcggtagata acttcgactt cacgcaggcc
      961 gttagcttca agcgtcgcgc cggtagagac caaatcgcag atagcgtcgg ccagccccgc
     1021 gcgcggcgcg acttcgacag aaccatttaa cagacacgat ttaaaagaga cgcctttctg
     1081 gtcgaggtag cgtttgagga ggtgcggata tgaggtagcg atacgtttac cgtccagcgc
     1141 ggccgggccg tcccaggctt cgtcaaccgg tgttgccagc gataaacggc agccgccgaa
     1201 gtcaagacgg cgcagggtta aatagcgtgg atcttcgccc tgtgcgcggc ggttgagtag
     1261 ctcttcttcc agcacgtttt cgccgataat accgagatcg accacgccat ccattaccag
     1321 acccggaatg tcatcatcac gcacgcgcag gatatcaatc ggcatgtttt ccgccatcgc
     1381 aatcaggcgc tgagtgtgta aattaatttt tatgccgcag cgggccagca attctcgtga
     1441 atcatcgctt aaacggcctg atttctgaat agctatgcgt aagcgggtgt tgtctaacat
     1501 tctgcgttcc tctttatcct gtctgaaccg gtctgtatcg cgcgccaaaa aaaaagcccc
     1561 cggaagatga tcttccgggg gctttctcat gcgttcatgc accactggaa gatccacagg
     1621 acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg
     1681 actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc
     1741 atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg
     1801 atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg
     1861 gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg
     1921 cgttagcaat ttaactgtga taaactaccg cattaaagct ctagaaggac cacctttgat
     1981 tgtaaatagt aataattacc acccttattt aattatttat ttaacttatt tatttattta
     2041 ttctacatat atacaaatct aataaagtga aaatctcccc cttcacattt ataattggcc
     2101 agtctttttc aaataagcat tccaaccagc atttctatac cttttacctt caatatctgg
     2161 atctcttcct ttaccaaaca atcctctacc aacaatgata atatcagttc cagtgctaac
     2221 aacttcatca acagttctat attgttgtcc taatccatca cctttatcat ctaatccaac
     2281 tccaggtgtc ataataagcc aatcaaatcc ttcttcttgg ccacccatat cacgttgggc
     2341 aataaatcca ataacaaatt ccttatcgga tttagcaatt tcaacagttt tttgagaata
     2401 ttctccatat gctaatgatc ccactgatga taattcagct aacatcaata accctcttgg
     2461 ctcttggttg gtggtggttt ctttagctcc ctgttttaat ccttcaacca ctccattccc
     2521 agtgacacca tgagcattgg taatatctgc ccaactacta attttataaa ctccaccaat
     2581 atattgtttc tttacggtat taccaatatc agcaaatttt ctatcttcaa aaatcataaa
     2641 ttgatgttta cgtgaaagtt ctaataatgg ttcaatagtg gattcatagg aaaaatcatt
     2701 gattatatca atatgagtct tgattaagca tacataagga cctaatttat caattaattc
     2761 aaggaattcc ttagtggtat caacgtcaat tgatgcacat aaattggttt tcttcagttc
     2821 cattaatcga aataatcgct gtgctactgg tgaggcatga gtttctgctc tctcactata
     2881 ggtcttagtg ttgactgtca tatcttttgt ttgctatttc tatttctatt tctttcttca
     2941 actgtaaaag gggcggtata taaaccagtg tgtatggggt tgttgctcag gaattgatca
     3001 atgtttaaaa gaatggttga aatcatcatt gaagaacaaa aaaaaagatt ttttctttca
     3061 ttatgttatc tgtataatat gcaggaaata agattgcaaa aaaatagccg tttttttgga
     3121 tactatcaaa caagaggaat gactaataca gaattagtgt tacgaatcaa tggcactaca
     3181 gcaactttca acaaaataat ctacgttcaa aatataatat atactattaa ataaaatcaa
     3241 ataataaaat aattttaaag aaagtagtac cagctctttt ttttgtttcc gtttatacca
     3301 tccaaatcaa ttcctattag tgtaccagaa tcgagctggc gccaagcgct ttcagtttct
     3361 ccatggatct tagggataac agggtaatgg atccatggtt tcccagaaca acgtttcgct
     3421 gctgaccatg tgcatcgcca cgcgctgttg ctcgcctgcc agcggcagaa ttgtcggcct
     3481 ttcggcgcct ggcagcaggg cgataacctc ttccaggcgt tcacttggcg cgtgcatcat
     3541 gatgtatttc gattcgcgcg cctgaatcac gccctgaata cgggtcagca atttatcgat
     3601 cagctcttgc ttgctctgtg ccatctcacc gtcgcgctga atcagacagg ctttagagcg
     3661 gtagataact tcgacttcac gcaggccgtt agcttcaagc gtcgcgccgg tagagaccaa
     3721 atcgcagata gcgtcggcca gccccgcgcg cggcgcgact tcgacagaac catttaacag
     3781 acacgattta aaagagacgc ctttctggtc gaggtagcgt ttgaggaggt gcggatatga
     3841 ggtagcgata cgtttaccgt ccagcgcggc cgggccgtcc caggcttcgt caaccggtgt
     3901 tgccagcgat aaacggcagc cgccgaagtc aagacggcgc agggttaaat agcgtggatc
     3961 ttcgccctgt gcgcggcggt tgagtagctc ttcttccagc acgttttcgc cgataatacc
     4021 gagatcgacc acgccatcca ttaccagacc cggaatgtca tcatcacgca cgcgcaggat
     4081 atcaatcggc atgttttccg ccatcgcaat caggcgctga gtgtgtaaat taatttttat
     4141 gccgcagcgg gccagcaatt ctcgtgaatc atcgcttaaa cggcctgatt tctgaatagc
     4201 tatgcgtaag cgggtgttgt ctaacattct gcgttcctct ttatcctgtc tgaaccggtc
     4261 tgtatcgcgc gccaaaaaaa aagcccccgg aagatgatct tccgggggct ttctcatgcg
     4321 ttcatgcacc actggaagat ctgggtaccg agctcgaatt cgtaatcatg gtcatagctg
     4381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4561 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     5221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5281 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5641 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     6541 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
