LOCUS       40924_29831        7412 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAT4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E, 
            Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L,
            Munoz-Dorado J.
  TITLE     Comprehensive Set of Integrative Plasmid Vectors for 
            Copper-Inducible Gene Expression in Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 78 (8), 2515-2521 (2012)
  PUBMED    22287008
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Treuner-Lange A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2011) Microbiology, University of Granada (Spain),
            Avda. Fuentenueva s/n, Granada, Granada 18071, Spain
REFERENCE   3  (bases 1 to 7412)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7412)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "8"; pages:
            "2515-2521"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-OCT-2011) Microbiology, University of Granada (Spain), Avda. 
            Fuentenueva s/n, Granada, Granada 18071, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7412
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..830
                     /label=cuoA promoter
                     /note="cuoA promoter"
                     /regulatory_class="promoter"
     misc_feature    831..859
                     /label=multicloning site
                     /note="multicloning site"
     primer_bind     complement(863..879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1444..2256)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_recomb     3040..6019
                     /label=att site
                     /note="att site"
     rep_origin      6130..6718
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6904..7046)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     protein_bind    7289..7310
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7325..7355
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7363..7379
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7387..7403
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcggcc atgaacggca cttcacggct ccgtggaacg acgctgctcc acgttccacc
       61 tgtgaccgtg acggttacag cgaaacccat ccgggtactc cggacaggtg ccgcgccacg
      121 gcaaccccgc atgaacaggc tcgctcagcc ctcggcacgc gttgtgctcc agcggaccga
      181 cttcacggga tgccggacgt cctcgagagc atcctcactc tgggagcaac caagcgatga
      241 agaagagcct gatgggactc cttgctgcac tccccctgat tgccgcggcc cccgcggtcg
      301 cgtgtgacga gcacgccaag cacggcgagc acgccagcca cggcgagcac gccaagcacg
      361 gcgatcattc cgcgctcgga ggcacggcca agaaggccgc tgcgcccgca cagaagcccg
      421 cctcggcccc ggccaccagg aatggcgtcc agaccgtgga cctcacggtg acccccaagg
      481 gcttcgagcc ctcggacgtg aaggtgaagg cggggcagcc cgtccgcctc gtggtcacgc
      541 gcaagacggc caagacgtgc gccacggaaa tcgtcatggc cgacctgggc atcaacaagc
      601 cgctccccat ggacacgccg gtgaccgtgg agttcacccc cagcaagtcg ggcacgctgc
      661 gctacgcctg cgcgatggac cacatcagcg ggctcgtcac catccagtag accgccgttc
      721 cgggcaggct gcgcaccgag gtcacgcgcc tcggggccgg gccacgccac cacggcggcc
      781 tggtcccctc ctcgcccgca gcttccatcc attcgtgaag aggcttcatg tctagactcg
      841 aggatccctg cagaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      901 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      961 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgcc
     1021 cctgtaatcc gggcagcgca acggaacatt catcagtgta aaaatggaat caataaagcc
     1081 ctgcgcagcg cgcagggtca gcctgaatac gcgtttaatg accagcacag tcgtgatggc
     1141 aaggtcagaa tagcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc
     1201 ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt
     1261 gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc
     1321 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaaagcc
     1381 ccccgcccgt caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga
     1441 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     1501 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     1561 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     1621 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     1681 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     1741 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     1801 cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     1861 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     1921 ttcttctaat acctggaatg ctgtttttcc ggggatcgca gtggtgagta accatgcatc
     1981 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     2041 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     2101 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     2161 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     2221 cctcgagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     2281 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
     2341 gagattttga gacacaacgt ggctttgttg aataaatcga acttttgctg agttgaagga
     2401 tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa agttcaaaat
     2461 caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca ctttctggct
     2521 ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac gaggcagacc
     2581 tcagcgctat tctgaccttg ccatcacgac tgtgctggtc attaaacgcg tattcaggct
     2641 gaccctgcgc gctgcgcagg gctttattga ttccattttt acactgatga atgttccgtt
     2701 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2761 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2821 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2881 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2941 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     3001 gtcagaccaa gtttactcat atatacttta gattgatttg gggatggagc cagacggccg
     3061 cgcttgtcga gcgcgacacg gcacagtggc ccaaggcaga gttggtggtg ccgtgacgac
     3121 gccaccgcgc cccatgcttt cgctgacggc gctccgcctc ctggtggagc gcccgcactc
     3181 gccgctggag ttgggcgccg cgctgggact ccagcgccgc tctacggagc gactgctggc
     3241 gcaactccgc gagacaggtg ttgaggtgga gcagacgcag gacgccgctg acgcgcggcg
     3301 ggttacgctg agcgtcagcc gggccgcagc gcttcgcgcg cttggcctca agtagtcgca
     3361 gtacccgact tgacagcacc cggtctccgg gtgcaggttg gcaagtacgc agtacctgga
     3421 ccggcagcac acggtgagcg gccaggacac acgacaactt cgagagtaga cgcgttccgg
     3481 cacgctgggc gcgcatctac tccggtgccc gacggtgctc ctcaccgtcg ccgtcggcgc
     3541 actgttctcc ggggttcgcc gtgcccgccc aactcaagac agcaacgagt cgagacgctg
     3601 ggcacggcga tacctcccgt gcggtcttga tacacccgtc cggactcgtg gactgcaatt
     3661 gcaatcaagg ttgcgattgc gttgtgttgc gtaaaaacac tgcgagcaaa ctcccgtcac
     3721 tcgggagagt cggtgttatt gcgcttgagt cgactaccgt gtgtcctcgc gtgtccgagt
     3781 caaggaaaat tgcatcgcgc gtattgaatt tagatgcgtg tttcatttat gtttctgatg
     3841 cgggctcaga tgaggacttg gagcctgcgt ccagccccac tgcgtgtgca tcggcccatg
     3901 tgcatgcagt ggggtgtcag tctgccgtgt tacctgacag ggcgtacagg cgtggtgcaa
     3961 cgggggcagt catcgtgagt gagattgaca ttgacgtgga ggccatcgtc gaaagagtcg
     4021 tccggcgggt gctgggcacc accgcgcaga agacggacga ggtgctcact cagactgagg
     4081 cggcagccct cgcaagtgtc agcacatcca ccattcgcaa ctggcaggcg cagggactcc
     4141 tgacggctgg gcatcgtggt cgcgtcaaga agtcggagtt ggaggcgttt ctcgcgtcgc
     4201 cgcgggcgaa ggctacgccc gaagaggagg ccgacgcgaa ggcagcggcc atcctggcgc
     4261 ggaaggcagg gcggcgccgt gggtaacgtc tatcgcaaga aggccactcg ctacttgtgg
     4321 atgtggtggt tcgacgctgc gggcgcgcgg cagtacgcga gcacgggcga agaagacgaa
     4381 gtgaaggcgc gcgccgtcct gaaggcgacc gagaagcgcg tggccgcgga gaaggaccag
     4441 ggactcggcg cgtccggtcc cgtcaccgtc gaggcctacg ccgccaagtg gatggagggg
     4501 cgcgtcacgc tgcgctcggt catggatgac cgctcccgga tgcagcacca cgtgctgccc
     4561 tcgttgggcg acaaggaact cggtgtgctg acgcgtcggc acgtgctcgc actggtgcgc
     4621 gaagtgcgcg caaagggcct ggcgccgcga acggtgcgcc acgtctacgc cctggtgcgg
     4681 acgatgctga gtgacgcggt ggtggacggg ctgctggagc gctcgccctg tgacttgcgc
     4741 gagcgccgcg gggagattcc ggccattcgg gatgctgacc ctcgatggcg ggactcggcc
     4801 gtctactcgc gggccgaggt cgtcgcactg attacggatg agcgcatccc ccttgagcgg
     4861 cgcgtgctct acgccgtggc cttcgtcgga gcggtgcgca tcggggaggc gtcagccctg
     4921 caggtccggg actgggacgg caccgtcaac cccttgggga agctctgggt gacgaagacc
     4981 ttcaaccaca aggtaggaga ggtctacgag accacgaaga cggacacgcc gcggcagatg
     5041 ccagtccacc cctggttgag cacgatgctt gccagttggc tcgcctacgg ctgggcgcgg
     5101 atgtacgccc ggacgccgcg ccaggatgac ctgattctcc cgagccccac cgggatgcac
     5161 cagcggcccc agggagtcct ccgtcgcctg catgccgacc tggacctcct ggggttcagg
     5221 cggagacgga ctcacgatgc tcgccggacg ttcatcagcc tggctcgtgc cgacggtgcc
     5281 gacaaatacc tactccagtg ggtcacccac ggcaggcgct ccgacatggt cgacgcctac
     5341 acgacgccgc cgtgggatgc gctctgtagg gaagtggcga agttgaagct cgaactgccc
     5401 aagcaggagg aggaaaccat ggcctgccga gtgccaagct gtgacagcgg tgtgacagcg
     5461 gacgctggaa aagaaaaacc ccagcaagtc cgagaacttg ctggggcagg ggtggcgagg
     5521 agtacgggac ttgaacccgt actcctgggc tccttctacg ccaatccgcg ccagttgcca
     5581 acaaaatctg agggcttaga cgcggagtcc gccaaactgg cacggactgg cgcagttgac
     5641 tggcacaagc ccgtgacagc gccccatccg cttgccgtgg tgcgtcagca gatgggggtc
     5701 ctcaagtccg gcgccggacg tgagcgcaag gtgctggcgt cccaactcgt ggacgcctcc
     5761 ctgatggccc tggagcggct tgcggccgcc ctgcaccgca cccatgccgc cgatgaccga
     5821 tggtccgact gcccggcctg ccgcgccgtg gcggacggcg agagcccttc cgctacctga
     5881 gtctcacccg cagtcaccgt cgacccaccc agccagagga gtcctccatg tcgcgaaagc
     5941 cgcgcagcac tacccgtccg cccgaagata gccccgatac gctccccgac cgcttcccgc
     6001 tcatggtcca ccgcatccca aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6061 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6121 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6181 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6241 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6301 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6361 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6421 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6481 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6541 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     6601 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     6661 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     6721 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     6781 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     6841 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     6901 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     6961 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatatac actccgctat
     7021 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     7081 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7141 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcca atacgcaaac
     7201 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7261 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7321 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7381 ttcacacagg aaacagctat gaccatgatt ac
//
