LOCUS       40924_29811        5742 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Episomal vector pMARS-mODC-ZA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Kitsera N, Khobta A, Epe B.
  TITLE     Destabilized green fluorescent protein detects rapid removal of 
            transcription blocks after genotoxic exposure
  JOURNAL   BioTechniques 43 (2), 222-227 (2007)
  PUBMED    17824390
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Khobta A, Kitsera N, Epe B.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2008) Institute of Pharmacy, Johannes Gutenberg 
            University of Mainz, Staudingerweg 5, Mainz 55099, Germany
REFERENCE   3  (bases 1 to 5742)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5742)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "2007"; volume: "43"; issue: "2"; pages: 
            "222-227"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JAN-2008) Institute of Pharmacy, Johannes Gutenberg University 
            of Mainz, Staudingerweg 5, Mainz 55099, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1329
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(1358..1477)
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQDDGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    2372..2493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2500..2955)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2982..3086
                     /label=AmpR promoter
     promoter        3306..3567
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             3638..4429
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4664..4711
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5040..5628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atcatagtac tcatctacac attgatccta gcagaagcac
     1381 aggctgcagg gtgacggtcc atcccgctct cctgggcaca agacatgggc agcgtgccat
     1441 catcctgctc ctccacctcc ggcgggaagc catggctctg gatctgcttc atgagagatc
     1501 tcgagctcaa gcttgcatgc ttctatatta ttttctaaaa gatttaaagt tttgccttct
     1561 ccatttagac ttataattca ctggaatttt tttgtgtgta tggtatgaca tatgggttcc
     1621 cttttatttt ttacatataa atatatttcc ctgtttttct aaaaaagagg ggatcctcta
     1681 gagtcgacct gcaggcatgc ttctatatta ttttctaaaa gatttaaagt tttgccttct
     1741 ccatttagac ttataattca ctggaatttt tttgtgtgta tggtatgaca tatgggttcc
     1801 cttttatttt ttacatataa atatatttcc ctgtttttct aaaaaagagg ggatcctcta
     1861 gagtcgacct gcaggcatgc ttctatatta ttttctaaaa gatttaaagt tttgccttct
     1921 ccatttagac ttataattca ctggaatttt tttgtgtgta tggtatgaca tatgggttcc
     1981 cttttatttt ttacatataa atatatttcc ctgtttttct aaaaaagagg ggatcctcta
     2041 gagtcgacct gcaggcatgc ttctatatta ttttctaaaa gatttaaagt tttgccttct
     2101 ccatttagac ttataattca ctggaatttt tttgtgtgta tggtatgaca tatgggttcc
     2161 cttttatttt ttacatataa atatatttcc ctgtttttct aaaaaagagg ggatcctgca
     2221 ggtcgactct agaggatccc cgggatccac cggatctaga taactgatca taatcagcca
     2281 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     2341 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     2401 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     2461 ttgtggtttg tccaaactca tcaatgtatc ttaacgcgta aattgtaagc gttaatattt
     2521 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     2581 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     2641 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     2701 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     2761 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     2821 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     2881 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     2941 cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3001 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     3061 ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg
     3121 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3181 tctcaattag tcagcaacca ggtgtgggga ctagggtgtg tttaggcgaa aagcggggct
     3241 tcggttgtac gcggttagga gtcccctcag gatatagtag tttcgctttt gcatagggag
     3301 ggggaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     3361 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     3421 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     3481 gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt
     3541 tgaccattca ccacattggt gtgcacctcc aaagcttgat atcgaggcct aggcttttgc
     3601 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
     3661 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
     3721 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
     3781 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc
     3841 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
     3901 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
     3961 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
     4021 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
     4081 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
     4141 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca
     4201 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     4261 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     4321 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     4381 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     4441 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     4501 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     4561 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact
     4621 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     4681 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg
     4741 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt
     4801 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg
     4861 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat
     4921 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4981 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5041 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5101 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5161 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     5221 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5281 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5341 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5401 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5461 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5521 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5581 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5641 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5701 ttatcccctg attctgtgga taaccgtatt accgccatgc at
//
