LOCUS       40924_29786        9184 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAMneo-CAT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9184)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9184)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 9184)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9184)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH; this vector has not been 
            completely sequenced. If you suspect there is an error in this 
            sequence, please contact CLONTECH's Technical Service Department at 
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail 
            TECH@CLONTECH.COM.'.
FEATURES             Location/Qualifiers
     source          1..9184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..1410
                     /label=MMTV
                     /note="Mouse mammary tumor virus long terminal repeat
                     (LTR)"
     CDS             1589..2245
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     intron          2423..2488
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2618..2638
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    3063..3197
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3210..3539
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3896..4687
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     intron          5111..5176
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5306..5326
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    5751..5885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6738..6842
                     /label=AmpR promoter
     CDS             6843..7700
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7874..8462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        9010..9184
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt
       61 tgaccattca ccacattggt gtgcacctcc aagcttgggc agaaatggtt gaactcccga
      121 gagtgtccta cacctagggg agaagcagcc aaggggttgt ttcccaccaa ggacgacccg
      181 tctgcgcaca aacggatgag cccatcagac aaagacatat tcattctctg ctgcaaactt
      241 ggcatagctc tgctttgcct ggggctattg ggggaagttg cggttcgtgc tcgcagggct
      301 ctcacccttg actcttttaa tagctcttct gtgcaagatt acaatctaaa caattcggag
      361 aactcgacct tcctcctgag gcaaggacca cagccaactt cctcttacaa gccgcatcga
      421 ttttgtcctt cagaaataga aataagaatg cttgctaaaa attatatttt taccaataag
      481 accaatccaa taggtagatt attagttact atgttaagaa atgaatcatt atcttttagt
      541 actattttta ctcaaattca gaagttagaa atgggaatag aaaatagaaa gagacgctca
      601 acctcaattg aagaacaggt gcaaggacta ttgaccacag gcctagaagt aaaaaaggga
      661 aaaaagagtg tttttgtcaa aataggagac aggtggtggc aaccagggac ttatagggga
      721 ccttacatct acagaccaac agatgccccc ttaccatata caggaagata tgacttaaat
      781 tgggataggt gggttacagt caatggctat aaagtgttat atagatccct cccttttcgt
      841 gaaagactcg ccagagctag acctccttgg tgtatgttgt ctcaagaaga aaaagacgac
      901 atgaaacaac aggtacatga ttatatttat ctaggaacag gaatgcactt ttggggaaag
      961 attttccata ccaaggaggg gacagtggct ggactaatag aacattattc tgcaaaaact
     1021 catggcatga gttattatga atagccttta ttggcccaac cttgcggttc ccagggctta
     1081 agtaagtttt tggttacaaa ctgttcttaa aacgaggatg tgagacaagt ggtttcctga
     1141 cttggtttgg tatcaaaggt tctgatctga gctctgagtg ttctattttc ctatgttctt
     1201 ttggaattta tccaaatctt atgtaaatgc ttatgtaaac caagatataa aagagtgctg
     1261 attttttgag taaacttgca acagtcctaa cattcacctc ttgtgtgttt gtgtctgttc
     1321 gccatcccgt ctccgctcgt cacttatcct tcactttcca gagggtcccc ccgcagaccc
     1381 cggcgaccct caggtcggcc gactgcggca gctggcgccc gaacagggac cctcggataa
     1441 gtgacccttg tctctatttc tactatttgg tgtttgtctt gtattgtctc tttcttgtct
     1501 ggctatcatc acaagagcgg aacggactca ccatagggac caagctagcc cggggtcgac
     1561 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
     1621 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     1681 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     1741 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     1801 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     1861 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     1921 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     1981 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     2041 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     2101 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     2161 tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     2221 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
     2281 gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgtcgacc
     2341 tcgagggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     2401 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     2461 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     2521 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     2581 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     2641 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     2701 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     2761 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     2821 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     2881 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     2941 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     3001 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     3061 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     3121 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     3181 cttatcatgt ctggatccgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3241 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3361 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3421 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     3481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     3541 agcttcacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
     3601 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     3661 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     3721 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     3781 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     3841 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
     3901 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     3961 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4021 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4081 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4141 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4201 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4261 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4321 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4381 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4441 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4501 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4561 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     4621 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     4681 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     4741 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     4801 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     4861 cccgggctcg atcccctcgc gagttggttc agctgctgcc tgaggctgga cgacctcgcg
     4921 gagttctacc ggcagtgcaa atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc
     4981 atccatgccc ccgaactgca ggagtgggga ggcacgatgg ccgctttggt cccggatctt
     5041 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     5101 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     5161 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     5221 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     5281 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     5341 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     5401 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     5461 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     5521 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     5581 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     5641 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     5701 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     5761 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5821 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5881 ggatccccag gaagctcctc tgtgtcctca taaaccctaa cctcctctac ttgagaggac
     5941 attccaatca taggctgccc atccaccctc tgtgtcctcc tgttaattag gtcacttaac
     6001 aaaaaggaaa ttgggtaggg gtttttcaca gaccgctttc taagggtaat tttaaaatat
     6061 ctgggaagtc ccttccactg ctgtgttcca gaagtgttgg taaacagccc acaaatgtca
     6121 acagcagaaa catacaagct gtcagctttg cacaagggcc caacaccctg ctcatcaaga
     6181 agcactgtgg ttgctgtgtt agtaatgtgc aaaacaggag gcacattttc cccacctgtg
     6241 taggttccaa aatatctagt gttttcattt ttacttggat caggaaccca gcactccact
     6301 ggataagcat tatccttatc caaaacagcc ttgtggtcag tgttcatctg ctgactgtca
     6361 actgtagcat tttttggggt tacagtttga gcaggatatt tggtcctgta gtttgctaac
     6421 acaccctgca gctccaaagg ttccccacca acagcaaaaa aatgaaaatt tgacccttga
     6481 atgggttttc cagcaccatt ttcatgagtt ttttgtgtcc ctgaatgcaa gtttaacata
     6541 gcagttaccc caataacctc agttttaaca gtaacagctt cccacatcaa aatatttcca
     6601 caggttaagt cctcatttaa attaggcaaa ggaattcttg aagacgaaag ggcctcgtga
     6661 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     6721 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6781 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6841 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6901 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6961 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7021 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     7081 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     7141 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     7201 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     7261 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7321 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7381 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7441 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7501 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7561 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7621 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7681 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7741 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7801 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7861 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7921 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7981 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     8041 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8101 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8161 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8221 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8281 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8341 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8401 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8461 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8521 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8581 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8641 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     8701 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     8761 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     8821 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     8881 atgtacgggc cagatatacg cgtatctgag gggactaggg tgtgtttagg cgaaaagcgg
     8941 ggcttcggtt gtacgcggtt aggagtcccc tcaggatata gtagtttcgc ttttgcatag
     9001 ggagggggaa atgtagtctt atgcaataca cttgtagtct tgcaacatgg taacgatgag
     9061 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
     9121 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
     9181 cact
//
