LOCUS       40924_29681        3836 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAK27, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Koronfel MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-AUG-2008) Crop Science, Faculty of Agriculture - Cairo
            University, Cairo University Street, Giza 12613, Egypt
REFERENCE   2  (bases 1 to 3836)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3836)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (18-AUG-2008) Crop Science, Faculty of Agriculture - Cairo 
            University, Cairo University Street, Giza 12613, Egypt"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    51..163
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(169..187)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(201..217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(358..813)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        840..944
                     /label=AmpR promoter
     CDS             945..1802
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1951..2763
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      2851..3439
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3727..3748
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3763..3793
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3801..3817
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaccatga ttacgccaag ctctaatacg actcactata gggaaagctc ggtaccacgc
       61 atgctgcaga cgcgttacgt atcggatcca gaattcgtcg acaagcttct cgagcctagg
      121 ctagctctag accacacgtg tgggggcccg agctcgcggc cgctgtattc tatagtgtca
      181 cctaaatggc cgcacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
      241 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
      301 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa
      361 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
      421 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
      481 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
      541 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
      601 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
      661 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
      721 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
      781 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
      841 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      901 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      961 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1141 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1321 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1801 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaacaat aaaactgtct
     1921 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc
     1981 tctaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2041 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca
     2101 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2161 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2221 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta
     2281 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     2341 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     2401 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     2461 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     2521 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     2581 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     2641 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     2701 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     2761 ttctaagaat taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2821 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2881 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2941 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     3001 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3061 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3121 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3181 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3241 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3301 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3361 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3421 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3481 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3541 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3601 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3661 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3721 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3781 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagct
//
