LOCUS       40924_29616        7830 bp DNA     circular SYN 18-DEC-2018
DEFINITION  CRIMIC donor vector pM37(2), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, 
            Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     A gene-specific T2A-GAL4 library for Drosophila
  JOURNAL   Elife 7, e35574 (2018)
  PUBMED    29565247
REFERENCE   2  (bases 1 to 7830)
  AUTHORS   Lee P-T., Zirin J, Kanca O, Lin W-W., Schulze KL, Li-Kroeger D, Tao 
            R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7830)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7830)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 7, 
            e35574 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2018) HHMI, Molecular "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    775..874
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     protein_bind    931..964
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    971..1070
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     CDS             1117..1170
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             1171..3813
                     /label=GAL4
                     /note="GAL4 transcriptional activator"
     regulatory      3832..3966
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    3832..3966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      4092..4297
                     /label=3XP3 promoter
                     /note="3XP3 promoter"
                     /regulatory_class="promoter"
     CDS             4325..5041
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    complement(5045..5179)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     5287..5320
                     /label=FRT (minimal)
                     /note="FRT (minimal)"
     protein_bind    5287..5320
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    complement(5377..5476)
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(5596..5612)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5642..5660)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5681..5697)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5705..5721)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5729..5759)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5774..5795)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6083..6671)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6845..7702)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7703..7807)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc gtctctaaac ggtctctaaa cgaagactta aacatgcatg tactgacgga
      721 cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg
      781 tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg
      841 ggggcgtagg gccgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg
      901 tgcttacgac cgtcagtcgc gcgagcgcga gaagttccta ttctctagaa agtataggaa
      961 cttcgaattg agtcgatcca acatggcgac ttgtcccatc cccggcatgt ttaaatatac
     1021 taattattct tgaactaatt ttaatcaacc gatttatctc tcttccgcag gtcgggaggt
     1081 tccggtggaa gcggaggtag cggcgaattc cgtacggagg gccgcggcag cctgctgacc
     1141 tgcggcgatg tggaggagaa ccccgggccc atgaagctgc tgagcagcat cgagcaggcc
     1201 tgcgatatct gccgcctgaa gaagctgaag tgcagcaagg agaagcccaa gtgcgccaag
     1261 tgcctgaaga acaactggga gtgccgctac agccccaaga ccaagcgcag ccccctgacc
     1321 cgcgcccacc tgaccgaggt ggagagccgc ctggagcgcc tggagcagct gttcctgctg
     1381 atcttccccc gcgaggatct ggatatgatc ctgaagatgg atagcctgca ggatatcaag
     1441 gccctgctga ccggcctgtt cgtgcaggat aacgtgaaca aggatgccgt gaccgatcgc
     1501 ctggccagcg tggaaaccga tatgcccctg accctgcgcc agcaccgcat cagcgccacc
     1561 agcagcagcg aggagagcag caacaagggc cagcgccagc tgaccgtgag catcgatagc
     1621 gccgcccacc acgataacag caccatcccc ctggatttca tgccccgcga tgccctgcac
     1681 ggcttcgatt ggagcgagga ggatgatatg agcgatggcc tgcccttcct gaaaaccgat
     1741 cccaacaaca acggcttctt cggcgatggc agcctgctgt gcatcctgcg cagcatcggc
     1801 ttcaagcccg agaactacac caacagcaac gtgaaccgcc tgcccaccat gatcaccgat
     1861 cgctacaccc tggccagccg cagcaccacc agccgcctgc tgcagagcta cctgaacaac
     1921 ttccacccct actgccccat cgtgcacagc cccaccctga tgatgctgta caacaaccag
     1981 atcgagatcg ccagcaagga tcagtggcag atcctgttca actgcatcct ggccatcggc
     2041 gcctggtgca tcgagggcga gagcaccgat atcgatgtgt tctactacca gaacgccaag
     2101 agccacctga ccagcaaggt gttcgagagc ggcagcatca tcctggtgac cgccctgcac
     2161 ctgctgagcc gctacaccca gtggcgccaa aagaccaaca ccagctacaa cttccacagc
     2221 ttcagcatcc gcatggccat cagcctgggc ctgaaccgcg atctgcccag cagcttcagc
     2281 gatagcagca tcctggagca gcgccgccgc atctggtgga gcgtgtacag ctgggagatc
     2341 cagctgagcc tgctgtacgg ccgcagcatc cagctgagcc agaacaccat cagcttcccc
     2401 agcagcgtgg atgatgtgca gcgcaccacc accggcccca ccatctacca cggcatcatc
     2461 gagactgccc gcctgctgca ggtgttcacc aagatctacg agctggataa gaccgtgacc
     2521 gccgagaaga gccccatctg cgccaagaag tgcctgatga tctgcaacga gatcgaggag
     2581 gtgagccgcc aggcccccaa gttcctgcag atggatatca gcaccaccgc cctgaccaac
     2641 ctgctgaagg agcacccctg gctgagcttc acccgcttcg agctgaagtg gaagcagctg
     2701 agcctgatca tctacgtgct gcgcgatttc ttcaccaact tcacccagaa gaagagccag
     2761 ctggagcagg atcagaacga tcaccagagc tacgaggtga agcgctgcag catcatgctg
     2821 agcgatgccg cccagcgcac cgtgatgagc gtgagcagct acatggataa ccacaacgtg
     2881 accccctact tcgcctggaa ctgcagctac tacctgttca acgccgtgct ggtgcccatc
     2941 aagaccctgc tgagcaacag caagagcaac gccgagaaca acgaaacggc ccagctgctg
     3001 cagcagatca acaccgtgct gatgctgctg aagaagctgg ccaccttcaa gatccagacc
     3061 tgcgagaagt acatccaggt gctggaggag gtgtgcgccc ccttcctgct gagccagtgc
     3121 gccatccccc tgccccacat cagctacaac aacagcaacg gcagcgccat caagaacatc
     3181 gtgggcagcg ccaccatcgc ccagtacccc accctgcccg aggagaacgt gaacaacatc
     3241 agcgtgaagt acgtgagccc cggcagcgtg ggacccagcc ccgtgcccct gaagagcggc
     3301 gccagcttca gcgatctggt gaagctgctg agcaaccgcc cccccagccg caacagcccc
     3361 gtgaccatcc cccgcagcac ccccagccac cgcagcgtga cccccttcct gggccagcag
     3421 cagcagctgc agagcctggt gcccctgacc cccagcgccc tgttcggcgg cgccaacttc
     3481 aaccagagcg gcaacatcgc cgatagcagc ctgagcttca ccttcaccaa cagcagcaac
     3541 ggccccaacc tgatcaccac ccagaccaac agccaggccc tgagccagcc catcgccagc
     3601 agcaacgtgc acgataactt catgaacaac gagatcaccg ccagcaagat cgatgatggc
     3661 aacaacagca agcccctgag ccccggctgg accgatcaga ccgcctacaa cgccttcggc
     3721 atcaccaccg gcatgttcaa caccaccacc atggatgatg tgtacaacta cctgttcgat
     3781 gatgaggata ccccccccaa ccccaagaag gagtaataag gcgcgcccaa agatccagac
     3841 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     3901 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     3961 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     4021 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcataattcg
     4081 agctcgcccg gggatctaat tcaattagag actaattcaa ttagagctaa ttcaattagg
     4141 atccaagctt atcgatttcg aaccctcgac cgccggagta taaatagagg cgcttcgtct
     4201 acggagcgac aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc
     4261 aaataaacaa gcgcagctga acaagctaaa caatcggact agagccggtc gccggccggc
     4321 caccatggtg tccaagggcg aggagctgtt caccggcgtg gtgccaattc tggtggagct
     4381 ggatggcgac gtgaacggcc acaagttcag cgtgtccggc gagggcgagg gcgacgccac
     4441 ctatggaaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccatggcc
     4501 aaccctcgtg accaccctga cctatggcgt gcagtgcttc agccgctacc ccgatcacat
     4561 gaagcagcac gatttcttca agagcgccat gcccgagggc tacgtgcagg agcgcaccat
     4621 ctttttcaag gatgacggca actacaagac ccgcgccgaa gtgaagttcg agggcgatac
     4681 cctcgtgaac cgcatcgagc tgaagggcat cgatttcaag gaggatggaa acatcctggg
     4741 ccacaagctg gagtacaact acaacagcca caacgtgtac atcatggccg acaagcagaa
     4801 gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggatggcg gcgtgcagct
     4861 ggccgatcac taccagcaga acaccccaat cggcgacggc ccagtgctgc tgcccgataa
     4921 ccattacctg agcacccaga gcgccctgag caaggatccc aacgagaagc gcgaccacat
     4981 ggtgctgctg gagtttgtga ccgccgccgg cattaccctg ggcatggatg agctgtacaa
     5041 gtaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     5101 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     5161 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     5221 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     5281 tgatcagaag ttcctattct ctagaaagta taggaacttc tcgcgctcgc gcgactgacg
     5341 gtcgtaagca cccgcgtacg tgtccacccc ggtcacaacc ccttgtgtca tgtcggcggc
     5401 cctacgcccc caactgagag aactcaaagg ttaccccagt tggggcacta ctcccgaaaa
     5461 ccgcttctga cctgggaaaa cgtgaagccc cggggcatcc gctgagggtt gccgccgggg
     5521 cttcggtgtg tccgtcagta cgcatgccgc gttgtcttcc gcgtgagacc cgcgtgagac
     5581 ggatatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc agcttttgtt
     5641 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5701 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5761 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5821 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5881 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5941 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6001 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6061 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6121 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6181 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6241 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6301 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6361 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6421 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6481 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6541 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6601 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6661 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6721 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6781 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6841 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6901 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6961 ccagtgctgc aatgataccg cgtgacccac gctcaccggc tccagattta tcagcaataa
     7021 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7081 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7141 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7201 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7261 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7321 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7381 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7441 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7501 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7561 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7621 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7681 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7741 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7801 ttccgcgcac atttccccga aaagtgccac
//
