LOCUS       40924_29611        7831 bp DNA     circular SYN 18-DEC-2018
DEFINITION  CRIMIC donor vector pM37(1), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7831)
  AUTHORS   Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, 
            Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     A gene-specific T2A-GAL4 library for Drosophila
  JOURNAL   Elife 7, e35574 (2018)
  PUBMED    29565247
REFERENCE   2  (bases 1 to 7831)
  AUTHORS   Lee P-T., Zirin J, Kanca O, Lin W-W., Schulze KL, Li-Kroeger D, Tao 
            R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7831)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 7, 
            e35574 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2018) HHMI, Molecular "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    775..874
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     protein_bind    931..964
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    971..1070
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     CDS             1118..1171
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             1172..3814
                     /label=GAL4
                     /note="GAL4 transcriptional activator"
     regulatory      3833..3967
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    3833..3967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      4093..4298
                     /label=3XP3 promoter
                     /note="3XP3 promoter"
                     /regulatory_class="promoter"
     CDS             4326..5042
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    complement(5046..5180)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     5288..5321
                     /label=FRT (minimal)
                     /note="FRT (minimal)"
     protein_bind    5288..5321
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    complement(5378..5477)
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(5597..5613)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5643..5661)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5682..5698)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5706..5722)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5730..5760)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5775..5796)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6084..6672)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6846..7703)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7704..7808)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc gtctctaaac ggtctctaaa cgaagactta aacatgcatg tactgacgga
      721 cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg
      781 tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg
      841 ggggcgtagg gccgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg
      901 tgcttacgac cgtcagtcgc gcgagcgcga gaagttccta ttctctagaa agtataggaa
      961 cttcgaattg agtcgatcca acatggcgac ttgtcccatc cccggcatgt ttaaatatac
     1021 taattattct tgaactaatt ttaatcaacc gatttatctc tcttccgcag gtggcggagg
     1081 ttccggtgga agcggaggta gcggcactag tacgcgtgag ggccgcggca gcctgctgac
     1141 ctgcggcgat gtggaggaga accccgggcc catgaagctg ctgagcagca tcgagcaggc
     1201 ctgcgatatc tgccgcctga agaagctgaa gtgcagcaag gagaagccca agtgcgccaa
     1261 gtgcctgaag aacaactggg agtgccgcta cagccccaag accaagcgca gccccctgac
     1321 ccgcgcccac ctgaccgagg tggagagccg cctggagcgc ctggagcagc tgttcctgct
     1381 gatcttcccc cgcgaggatc tggatatgat cctgaagatg gatagcctgc aggatatcaa
     1441 ggccctgctg accggcctgt tcgtgcagga taacgtgaac aaggatgccg tgaccgatcg
     1501 cctggccagc gtggaaaccg atatgcccct gaccctgcgc cagcaccgca tcagcgccac
     1561 cagcagcagc gaggagagca gcaacaaggg ccagcgccag ctgaccgtga gcatcgatag
     1621 cgccgcccac cacgataaca gcaccatccc cctggatttc atgccccgcg atgccctgca
     1681 cggcttcgat tggagcgagg aggatgatat gagcgatggc ctgcccttcc tgaaaaccga
     1741 tcccaacaac aacggcttct tcggcgatgg cagcctgctg tgcatcctgc gcagcatcgg
     1801 cttcaagccc gagaactaca ccaacagcaa cgtgaaccgc ctgcccacca tgatcaccga
     1861 tcgctacacc ctggccagcc gcagcaccac cagccgcctg ctgcagagct acctgaacaa
     1921 cttccacccc tactgcccca tcgtgcacag ccccaccctg atgatgctgt acaacaacca
     1981 gatcgagatc gccagcaagg atcagtggca gatcctgttc aactgcatcc tggccatcgg
     2041 cgcctggtgc atcgagggcg agagcaccga tatcgatgtg ttctactacc agaacgccaa
     2101 gagccacctg accagcaagg tgttcgagag cggcagcatc atcctggtga ccgccctgca
     2161 cctgctgagc cgctacaccc agtggcgcca aaagaccaac accagctaca acttccacag
     2221 cttcagcatc cgcatggcca tcagcctggg cctgaaccgc gatctgccca gcagcttcag
     2281 cgatagcagc atcctggagc agcgccgccg catctggtgg agcgtgtaca gctgggagat
     2341 ccagctgagc ctgctgtacg gccgcagcat ccagctgagc cagaacacca tcagcttccc
     2401 cagcagcgtg gatgatgtgc agcgcaccac caccggcccc accatctacc acggcatcat
     2461 cgagactgcc cgcctgctgc aggtgttcac caagatctac gagctggata agaccgtgac
     2521 cgccgagaag agccccatct gcgccaagaa gtgcctgatg atctgcaacg agatcgagga
     2581 ggtgagccgc caggccccca agttcctgca gatggatatc agcaccaccg ccctgaccaa
     2641 cctgctgaag gagcacccct ggctgagctt cacccgcttc gagctgaagt ggaagcagct
     2701 gagcctgatc atctacgtgc tgcgcgattt cttcaccaac ttcacccaga agaagagcca
     2761 gctggagcag gatcagaacg atcaccagag ctacgaggtg aagcgctgca gcatcatgct
     2821 gagcgatgcc gcccagcgca ccgtgatgag cgtgagcagc tacatggata accacaacgt
     2881 gaccccctac ttcgcctgga actgcagcta ctacctgttc aacgccgtgc tggtgcccat
     2941 caagaccctg ctgagcaaca gcaagagcaa cgccgagaac aacgaaacgg cccagctgct
     3001 gcagcagatc aacaccgtgc tgatgctgct gaagaagctg gccaccttca agatccagac
     3061 ctgcgagaag tacatccagg tgctggagga ggtgtgcgcc cccttcctgc tgagccagtg
     3121 cgccatcccc ctgccccaca tcagctacaa caacagcaac ggcagcgcca tcaagaacat
     3181 cgtgggcagc gccaccatcg cccagtaccc caccctgccc gaggagaacg tgaacaacat
     3241 cagcgtgaag tacgtgagcc ccggcagcgt gggacccagc cccgtgcccc tgaagagcgg
     3301 cgccagcttc agcgatctgg tgaagctgct gagcaaccgc ccccccagcc gcaacagccc
     3361 cgtgaccatc ccccgcagca cccccagcca ccgcagcgtg acccccttcc tgggccagca
     3421 gcagcagctg cagagcctgg tgcccctgac ccccagcgcc ctgttcggcg gcgccaactt
     3481 caaccagagc ggcaacatcg ccgatagcag cctgagcttc accttcacca acagcagcaa
     3541 cggccccaac ctgatcacca cccagaccaa cagccaggcc ctgagccagc ccatcgccag
     3601 cagcaacgtg cacgataact tcatgaacaa cgagatcacc gccagcaaga tcgatgatgg
     3661 caacaacagc aagcccctga gccccggctg gaccgatcag accgcctaca acgccttcgg
     3721 catcaccacc ggcatgttca acaccaccac catggatgat gtgtacaact acctgttcga
     3781 tgatgaggat acccccccca accccaagaa ggagtaataa ggcgcgccca aagatccaga
     3841 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     3901 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     3961 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     4021 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcataattc
     4081 gagctcgccc ggggatctaa ttcaattaga gactaattca attagagcta attcaattag
     4141 gatccaagct tatcgatttc gaaccctcga ccgccggagt ataaatagag gcgcttcgtc
     4201 tacggagcga caattcaatt caaacaagca aagtgaacac gtcgctaagc gaaagctaag
     4261 caaataaaca agcgcagctg aacaagctaa acaatcggac tagagccggt cgccggccgg
     4321 ccaccatggt gtccaagggc gaggagctgt tcaccggcgt ggtgccaatt ctggtggagc
     4381 tggatggcga cgtgaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgacgcca
     4441 cctatggaaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccatggc
     4501 caaccctcgt gaccaccctg acctatggcg tgcagtgctt cagccgctac cccgatcaca
     4561 tgaagcagca cgatttcttc aagagcgcca tgcccgaggg ctacgtgcag gagcgcacca
     4621 tctttttcaa ggatgacggc aactacaaga cccgcgccga agtgaagttc gagggcgata
     4681 ccctcgtgaa ccgcatcgag ctgaagggca tcgatttcaa ggaggatgga aacatcctgg
     4741 gccacaagct ggagtacaac tacaacagcc acaacgtgta catcatggcc gacaagcaga
     4801 agaacggcat caaggccaac ttcaagatcc gccacaacat cgaggatggc ggcgtgcagc
     4861 tggccgatca ctaccagcag aacaccccaa tcggcgacgg cccagtgctg ctgcccgata
     4921 accattacct gagcacccag agcgccctga gcaaggatcc caacgagaag cgcgaccaca
     4981 tggtgctgct ggagtttgtg accgccgccg gcattaccct gggcatggat gagctgtaca
     5041 agtaggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     5101 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     5161 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     5221 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     5281 atgatcagaa gttcctattc tctagaaagt ataggaactt ctcgcgctcg cgcgactgac
     5341 ggtcgtaagc acccgcgtac gtgtccaccc cggtcacaac cccttgtgtc atgtcggcgg
     5401 ccctacgccc ccaactgaga gaactcaaag gttaccccag ttggggcact actcccgaaa
     5461 accgcttctg acctgggaaa acgtgaagcc ccggggcatc cgctgagggt tgccgccggg
     5521 gcttcggtgt gtccgtcagt acgcatgccg cgttgtcttc cgcgtgagac ccgcgtgaga
     5581 cggatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt
     5641 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     5701 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5761 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5821 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5881 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5941 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6001 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6061 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6121 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6181 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6241 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6301 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6361 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6421 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6481 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6541 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6601 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6661 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6721 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6781 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6841 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6901 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6961 cccagtgctg caatgatacc gcgtgaccca cgctcaccgg ctccagattt atcagcaata
     7021 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7081 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7141 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7201 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7261 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7321 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7381 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7441 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7501 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7561 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7621 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7681 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7741 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7801 gttccgcgca catttccccg aaaagtgcca c
//
