LOCUS       40924_29606        7829 bp DNA     circular SYN 18-DEC-2018
DEFINITION  CRIMIC donor vector pM37(0), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, 
            Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     A gene-specific T2A-GAL4 library for Drosophila
  JOURNAL   Elife 7, e35574 (2018)
  PUBMED    29565247
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Lee P-T., Zirin J, Kanca O, Lin W-W., Schulze KL, Li-Kroeger D, Tao 
            R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7829)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7829)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 7, 
            e35574 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2018) HHMI, Molecular "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    775..874
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     protein_bind    931..964
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    971..1070
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     CDS             1116..1169
                     /label=T2A
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             1170..3812
                     /label=GAL4
                     /note="GAL4 transcriptional activator"
     regulatory      3831..3965
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     polyA_signal    3831..3965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      4091..4296
                     /label=3XP3 promoter
                     /note="3XP3 promoter"
                     /regulatory_class="promoter"
     CDS             4324..5040
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    complement(5044..5178)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     5286..5319
                     /label=FRT (minimal)
                     /note="FRT (minimal)"
     protein_bind    5286..5319
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    complement(5376..5475)
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(5595..5611)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5641..5659)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5680..5696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5704..5720)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5728..5758)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5773..5794)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6082..6670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6844..7701)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7702..7806)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc gtctctaaac ggtctctaaa cgaagactta aacatgcatg tactgacgga
      721 cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg
      781 tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg
      841 ggggcgtagg gccgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg
      901 tgcttacgac cgtcagtcgc gcgagcgcga gaagttccta ttctctagaa agtataggaa
      961 cttcgaattg agtcgatcca acatggcgac ttgtcccatc cccggcatgt ttaaatatac
     1021 taattattct tgaactaatt ttaatcaacc gatttatctc tcttccgcag gtgggaggtt
     1081 ccggtggaag cggaggtagc ggcaccggtg catgcgaggg ccgcggcagc ctgctgacct
     1141 gcggcgatgt ggaggagaac cccgggccca tgaagctgct gagcagcatc gagcaggcct
     1201 gcgatatctg ccgcctgaag aagctgaagt gcagcaagga gaagcccaag tgcgccaagt
     1261 gcctgaagaa caactgggag tgccgctaca gccccaagac caagcgcagc cccctgaccc
     1321 gcgcccacct gaccgaggtg gagagccgcc tggagcgcct ggagcagctg ttcctgctga
     1381 tcttcccccg cgaggatctg gatatgatcc tgaagatgga tagcctgcag gatatcaagg
     1441 ccctgctgac cggcctgttc gtgcaggata acgtgaacaa ggatgccgtg accgatcgcc
     1501 tggccagcgt ggaaaccgat atgcccctga ccctgcgcca gcaccgcatc agcgccacca
     1561 gcagcagcga ggagagcagc aacaagggcc agcgccagct gaccgtgagc atcgatagcg
     1621 ccgcccacca cgataacagc accatccccc tggatttcat gccccgcgat gccctgcacg
     1681 gcttcgattg gagcgaggag gatgatatga gcgatggcct gcccttcctg aaaaccgatc
     1741 ccaacaacaa cggcttcttc ggcgatggca gcctgctgtg catcctgcgc agcatcggct
     1801 tcaagcccga gaactacacc aacagcaacg tgaaccgcct gcccaccatg atcaccgatc
     1861 gctacaccct ggccagccgc agcaccacca gccgcctgct gcagagctac ctgaacaact
     1921 tccaccccta ctgccccatc gtgcacagcc ccaccctgat gatgctgtac aacaaccaga
     1981 tcgagatcgc cagcaaggat cagtggcaga tcctgttcaa ctgcatcctg gccatcggcg
     2041 cctggtgcat cgagggcgag agcaccgata tcgatgtgtt ctactaccag aacgccaaga
     2101 gccacctgac cagcaaggtg ttcgagagcg gcagcatcat cctggtgacc gccctgcacc
     2161 tgctgagccg ctacacccag tggcgccaaa agaccaacac cagctacaac ttccacagct
     2221 tcagcatccg catggccatc agcctgggcc tgaaccgcga tctgcccagc agcttcagcg
     2281 atagcagcat cctggagcag cgccgccgca tctggtggag cgtgtacagc tgggagatcc
     2341 agctgagcct gctgtacggc cgcagcatcc agctgagcca gaacaccatc agcttcccca
     2401 gcagcgtgga tgatgtgcag cgcaccacca ccggccccac catctaccac ggcatcatcg
     2461 agactgcccg cctgctgcag gtgttcacca agatctacga gctggataag accgtgaccg
     2521 ccgagaagag ccccatctgc gccaagaagt gcctgatgat ctgcaacgag atcgaggagg
     2581 tgagccgcca ggcccccaag ttcctgcaga tggatatcag caccaccgcc ctgaccaacc
     2641 tgctgaagga gcacccctgg ctgagcttca cccgcttcga gctgaagtgg aagcagctga
     2701 gcctgatcat ctacgtgctg cgcgatttct tcaccaactt cacccagaag aagagccagc
     2761 tggagcagga tcagaacgat caccagagct acgaggtgaa gcgctgcagc atcatgctga
     2821 gcgatgccgc ccagcgcacc gtgatgagcg tgagcagcta catggataac cacaacgtga
     2881 ccccctactt cgcctggaac tgcagctact acctgttcaa cgccgtgctg gtgcccatca
     2941 agaccctgct gagcaacagc aagagcaacg ccgagaacaa cgaaacggcc cagctgctgc
     3001 agcagatcaa caccgtgctg atgctgctga agaagctggc caccttcaag atccagacct
     3061 gcgagaagta catccaggtg ctggaggagg tgtgcgcccc cttcctgctg agccagtgcg
     3121 ccatccccct gccccacatc agctacaaca acagcaacgg cagcgccatc aagaacatcg
     3181 tgggcagcgc caccatcgcc cagtacccca ccctgcccga ggagaacgtg aacaacatca
     3241 gcgtgaagta cgtgagcccc ggcagcgtgg gacccagccc cgtgcccctg aagagcggcg
     3301 ccagcttcag cgatctggtg aagctgctga gcaaccgccc ccccagccgc aacagccccg
     3361 tgaccatccc ccgcagcacc cccagccacc gcagcgtgac ccccttcctg ggccagcagc
     3421 agcagctgca gagcctggtg cccctgaccc ccagcgccct gttcggcggc gccaacttca
     3481 accagagcgg caacatcgcc gatagcagcc tgagcttcac cttcaccaac agcagcaacg
     3541 gccccaacct gatcaccacc cagaccaaca gccaggccct gagccagccc atcgccagca
     3601 gcaacgtgca cgataacttc atgaacaacg agatcaccgc cagcaagatc gatgatggca
     3661 acaacagcaa gcccctgagc cccggctgga ccgatcagac cgcctacaac gccttcggca
     3721 tcaccaccgg catgttcaac accaccacca tggatgatgt gtacaactac ctgttcgatg
     3781 atgaggatac cccccccaac cccaagaagg agtaataagg cgcgcccaaa gatccagaca
     3841 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3901 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3961 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     4021 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cataattcga
     4081 gctcgcccgg ggatctaatt caattagaga ctaattcaat tagagctaat tcaattagga
     4141 tccaagctta tcgatttcga accctcgacc gccggagtat aaatagaggc gcttcgtcta
     4201 cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca
     4261 aataaacaag cgcagctgaa caagctaaac aatcggacta gagccggtcg ccggccggcc
     4321 accatggtgt ccaagggcga ggagctgttc accggcgtgg tgccaattct ggtggagctg
     4381 gatggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgacgccacc
     4441 tatggaaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccatggcca
     4501 accctcgtga ccaccctgac ctatggcgtg cagtgcttca gccgctaccc cgatcacatg
     4561 aagcagcacg atttcttcaa gagcgccatg cccgagggct acgtgcagga gcgcaccatc
     4621 tttttcaagg atgacggcaa ctacaagacc cgcgccgaag tgaagttcga gggcgatacc
     4681 ctcgtgaacc gcatcgagct gaagggcatc gatttcaagg aggatggaaa catcctgggc
     4741 cacaagctgg agtacaacta caacagccac aacgtgtaca tcatggccga caagcagaag
     4801 aacggcatca aggccaactt caagatccgc cacaacatcg aggatggcgg cgtgcagctg
     4861 gccgatcact accagcagaa caccccaatc ggcgacggcc cagtgctgct gcccgataac
     4921 cattacctga gcacccagag cgccctgagc aaggatccca acgagaagcg cgaccacatg
     4981 gtgctgctgg agtttgtgac cgccgccggc attaccctgg gcatggatga gctgtacaag
     5041 taggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     5101 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     5161 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     5221 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     5281 gatcagaagt tcctattctc tagaaagtat aggaacttct cgcgctcgcg cgactgacgg
     5341 tcgtaagcac ccgcgtacgt gtccaccccg gtcacaaccc cttgtgtcat gtcggcggcc
     5401 ctacgccccc aactgagaga actcaaaggt taccccagtt ggggcactac tcccgaaaac
     5461 cgcttctgac ctgggaaaac gtgaagcccc ggggcatccg ctgagggttg ccgccggggc
     5521 ttcggtgtgt ccgtcagtac gcatgccgcg ttgtcttccg cgtgagaccc gcgtgagacg
     5581 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca gcttttgttc
     5641 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     5701 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     5761 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     5821 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     5881 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     5941 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6001 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6061 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     6301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6481 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6961 cagtgctgca atgataccgc gtgacccacg ctcaccggct ccagatttat cagcaataaa
     7021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7801 tccgcgcaca tttccccgaa aagtgccac
//
