LOCUS       40924_29601        5337 bp DNA     circular SYN 18-DEC-2018
DEFINITION  CRIMIC donor vector pM36, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, 
            Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     A gene-specific T2A-GAL4 library for Drosophila
  JOURNAL   Elife 7, e35574 (2018)
  PUBMED    29565247
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Lee P-T., Zirin J, Kanca O, Lin W-W., Schulze KL, Li-Kroeger D, Tao 
            R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5337)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5337)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 7, 
            e35574 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2018) HHMI, Molecular "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    798..897
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     misc_feature    960..1059
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     regulatory      1060..1077
                     /label=stop codons in all reading frames
                     /note="stop codons in all reading frames"
                     /regulatory_class="terminator"
     polyA_signal    1078..1212
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1082..1273
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     regulatory      1320..1585
                     /label=3XP3 promoter
                     /note="3XP3 promoter"
                     /regulatory_class="promoter"
     promoter        1591..1828
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             1841..2557
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    complement(2561..2695)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2859..2958)
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(3103..3119)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3149..3167)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3188..3204)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3212..3228)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3236..3266)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3281..3302)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3590..4178)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4352..5209)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5210..5314)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc gtctctaacg gtctctaacg aagacttaaa catgcatcga tcgtctggta
      721 ctacattcac gcgtactgac ggacacaccg aagccccggc ggcaaccctc agcggatgcc
      781 ccggggcttc acgttttccc aggtcagaag cggttttcgg gagtagtgcc ccaactgggg
      841 taacctttga gttctctcag ttgggggcgt agggccgccg acatgacaca aggggttgtg
      901 accggggtgg acacgtacgc gggtgcttac gaccgtcagt cgcgcgagcg cgagaattca
      961 gtcgatccaa catggcgact tgtcccatcc ccggcatgtt taaatatact aattattctt
     1021 gaactaattt taatcaaccg atttatctct cttccgcagg tctaactaac taacaaagat
     1081 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     1141 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     1201 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     1261 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcat
     1321 aattcgagct cgcccgggga tctaattcaa ttagagacta attcaattag agctaattca
     1381 attaggatcc aagcttatcg atttcgaacc ctcgaccgcc ggagtataaa tagaggcgct
     1441 tcgtctacgg agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag
     1501 ctaagcaaat aaacaagcgc agctgaacaa gctaaacaat cggggtaccg ctagagtcga
     1561 cggtaccgcg ggcccgggat ccaccgagcg ccggagtata aatagaggcg cttcgtctac
     1621 ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa
     1681 ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt taaagtgaat
     1741 caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc tgccaagaag
     1801 taattattga atacaagaag agaactctcg gccggccacc atggtgtcca agggcgagga
     1861 gctgttcacc ggcgtggtgc caattctggt ggagctggat ggcgacgtga acggccacaa
     1921 gttcagcgtg tccggcgagg gcgagggcga cgccacctat ggaaagctga ccctgaagtt
     1981 catctgcacc accggcaagc tgcccgtgcc atggccaacc ctcgtgacca ccctgaccta
     2041 tggcgtgcag tgcttcagcc gctaccccga tcacatgaag cagcacgatt tcttcaagag
     2101 cgccatgccc gagggctacg tgcaggagcg caccatcttt ttcaaggatg acggcaacta
     2161 caagacccgc gccgaagtga agttcgaggg cgataccctc gtgaaccgca tcgagctgaa
     2221 gggcatcgat ttcaaggagg atggaaacat cctgggccac aagctggagt acaactacaa
     2281 cagccacaac gtgtacatca tggccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     2341 gatccgccac aacatcgagg atggcggcgt gcagctggcc gatcactacc agcagaacac
     2401 cccaatcggc gacggcccag tgctgctgcc cgataaccat tacctgagca cccagagcgc
     2461 cctgagcaag gatcccaacg agaagcgcga ccacatggtg ctgctggagt ttgtgaccgc
     2521 cgccggcatt accctgggca tggatgagct gtacaagtag gatccagaca tgataagata
     2581 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     2641 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     2701 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     2761 caagtaaaac ctctacaaat gtggtatggc tgattatgat catcgcgctc gcgcgactga
     2821 cggtcgtaag cacccgcgta cgtgtccacc ccggtcacaa ccccttgtgt catgtcggcg
     2881 gccctacgcc cccaactgag agaactcaaa ggttacccca gttggggcac tactcccgaa
     2941 aaccgcttct gacctgggaa aacgtgaagc cccggggcat ccgctgaggg ttgccgccgg
     3001 ggcttcggtg tgtccgtcag tacggtacta cctctcgtcc ttcactgcgc atgccgcgtt
     3061 gtcttccgcg tgagacccgc gtgagacgga tatcaagctt atcgataccg tcgacctcga
     3121 gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
     3181 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3241 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3301 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3361 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     3421 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3481 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3541 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3601 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3661 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3721 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3781 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3841 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3901 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3961 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4021 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4081 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     4141 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     4201 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     4261 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4321 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4381 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4441 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgt gacccacgct
     4501 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4561 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4621 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4681 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4741 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4801 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4861 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4921 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     4981 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5041 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5101 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5161 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5221 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     5281 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//
