LOCUS       40924_29596        5357 bp DNA     circular SYN 18-DEC-2018
DEFINITION  CRIMIC donor vector pM14, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, 
            Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     A gene-specific T2A-GAL4 library for Drosophila
  JOURNAL   Elife 7, e35574 (2018)
  PUBMED    29565247
REFERENCE   2  (bases 1 to 5357)
  AUTHORS   Lee P-T., Zirin J, Kanca O, Lin W-W., Schulze KL, Li-Kroeger D, Tao 
            R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, 
            Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N,
            Bellen HJ.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 5357)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5357)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife 7, 
            e35574 (2018)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-AUG-2018) HHMI, Molecular "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    775..874
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     protein_bind    931..964
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     misc_feature    971..1070
                     /label=MHC splice acceptor site
                     /note="MHC splice acceptor site"
     regulatory      1071..1088
                     /label=stop codons in all reading frames
                     /note="stop codons in all reading frames"
                     /regulatory_class="terminator"
     polyA_signal    1089..1223
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      1093..1284
                     /label=SV40 polyadenylation signal
                     /note="SV40 polyadenylation signal"
                     /regulatory_class="polyA_signal_sequence"
     regulatory      1331..1596
                     /label=3XP3 promoter
                     /note="3XP3 promoter"
                     /regulatory_class="promoter"
     promoter        1602..1839
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             1852..2568
                     /label=EGFP
                     /note="enhanced GFP"
     polyA_signal    complement(2572..2706)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     2814..2847
                     /label=FRT (minimal)
                     /note="FRT (minimal)"
     protein_bind    2814..2847
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    complement(2904..3003)
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     primer_bind     complement(3123..3139)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3169..3187)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3208..3224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3232..3248)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3256..3286)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3301..3322)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3610..4198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4372..5229)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5230..5334)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc gtctctaaac ggtctctaaa cgaagactta aacatgcatg tactgacgga
      721 cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg
      781 tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg
      841 ggggcgtagg gccgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg
      901 tgcttacgac cgtcagtcgc gcgagcgcga gaagttccta ttctctagaa agtataggaa
      961 cttcgaattc agtcgatcca acatggcgac ttgtcccatc cccggcatgt ttaaatatac
     1021 taattattct tgaactaatt ttaatcaacc gatttatctc tcttccgcag gtctaactaa
     1081 ctaacaaaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1141 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1201 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1261 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     1321 attatgatca taattcgagc tcgcccgggg atctaattca attagagact aattcaatta
     1381 gagctaattc aattaggatc caagcttatc gatttcgaac cctcgaccgc cggagtataa
     1441 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     1501 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tcggggtacc
     1561 gctagagtcg acggtaccgc gggcccggga tccaccgagc gccggagtat aaatagaggc
     1621 gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga
     1681 aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag
     1741 ttaaagtgaa tcaattaaaa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat
     1801 ctgccaagaa gtaattattg aatacaagaa gagaactctc ggccggccac catggtgtcc
     1861 aagggcgagg agctgttcac cggcgtggtg ccaattctgg tggagctgga tggcgacgtg
     1921 aacggccaca agttcagcgt gtccggcgag ggcgagggcg acgccaccta tggaaagctg
     1981 accctgaagt tcatctgcac caccggcaag ctgcccgtgc catggccaac cctcgtgacc
     2041 accctgacct atggcgtgca gtgcttcagc cgctaccccg atcacatgaa gcagcacgat
     2101 ttcttcaaga gcgccatgcc cgagggctac gtgcaggagc gcaccatctt tttcaaggat
     2161 gacggcaact acaagacccg cgccgaagtg aagttcgagg gcgataccct cgtgaaccgc
     2221 atcgagctga agggcatcga tttcaaggag gatggaaaca tcctgggcca caagctggag
     2281 tacaactaca acagccacaa cgtgtacatc atggccgaca agcagaagaa cggcatcaag
     2341 gccaacttca agatccgcca caacatcgag gatggcggcg tgcagctggc cgatcactac
     2401 cagcagaaca ccccaatcgg cgacggccca gtgctgctgc ccgataacca ttacctgagc
     2461 acccagagcg ccctgagcaa ggatcccaac gagaagcgcg accacatggt gctgctggag
     2521 tttgtgaccg ccgccggcat taccctgggc atggatgagc tgtacaagta ggatccagac
     2581 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2641 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2701 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     2761 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagaagttc
     2821 ctattctcta gaaagtatag gaacttctcg cgctcgcgcg actgacggtc gtaagcaccc
     2881 gcgtacgtgt ccaccccggt cacaacccct tgtgtcatgt cggcggccct acgcccccaa
     2941 ctgagagaac tcaaaggtta ccccagttgg ggcactactc ccgaaaaccg cttctgacct
     3001 gggaaaacgt gaagccccgg ggcatccgct gagggttgcc gccggggctt cggtgtgtcc
     3061 gtcagtacgc atgccgcgtt gtcttccgcg tgagacccgc gtgagacgga tatcaagctt
     3121 atcgataccg tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg
     3181 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     3241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     3301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3361 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3421 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     3481 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     3541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     3601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     3661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     3721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4021 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     4081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4321 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     4381 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     4441 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     4501 gataccgcgt gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     4561 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     4621 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     4681 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     4741 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4801 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4861 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4921 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4981 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5041 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5101 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5161 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5221 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5281 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     5341 tccccgaaaa gtgccac
//
