LOCUS       62056_16385       13444 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13444)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..766
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             976..5076
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKQIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     terminator      6513..6765
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6813..6837
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(6965..8307)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        8566..8847
                     /label=TRP1 promoter
     CDS             8848..9519
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(9625..10080)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(10199..11344)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MAQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(11646..12437)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHLERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      complement(12646..13277)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    13357..13381
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagcttgcat gcctgcaggt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
       61 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      121 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      181 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
      241 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
      301 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa catggtggag
      361 cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca
      421 attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct
      481 atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat
      541 tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga
      601 cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa
      661 gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg
      721 caagaccctt cctctatata aggaagttca tttcatttgg agaggagagc aatcagtgca
      781 aacaaaaacc ttaatccaga catcttgaaa ttaatcatat aaccattata atctgagcag
      841 acgtataaga ctagtatgga ttacaaggac cacgacggtg attataagga tcacgacatc
      901 gactacaagg acgatgatga caagatggct ccgaagaaga aaaggaaagt gggtatacac
      961 ggtgttccag cagccgataa gaagtacagt atcggacttg acatagggac taactctgtc
     1021 gggtgggcag taatcacaga tgagtacaaa gtcccatcta agaagttcaa ggtgctcggt
     1081 aacaccgata gacacagcat caagaagaac ctcatcggtg ctctcctgtt cgattccggc
     1141 gaaacggccg aagccactag gttgaagagg acggcccgac gacgttatac ccgtagaaag
     1201 aataggatat gctatctgca ggagatattc tccaacgaga tggctaaggt agacgatagc
     1261 ttcttccatc gtctcgagga gtccttcctg gtggaagagg acaagaaaca tgaaagacac
     1321 ccaatctttg ggaacattgt tgacgaggtc gcatatcatg agaagtaccc aacaatctac
     1381 cacttgagga agaaactcgt cgacagtact gacaaggctg atctgcgttt aatctactta
     1441 gcactggctc atatgatcaa gttcagaggt cacttcttga tcgagggtga cttgaaccct
     1501 gacaattcag acgtagacaa gctgttcatc cagttagtcc agacgtataa tcagttgttc
     1561 gaggaaaacc caattaacgc tagtggagtc gacgccaagg caatattgag tgcaagactc
     1621 tccaagtccc gaaggttgga aaacctcatt gctcaattgc ctggagagaa gaagaacggg
     1681 ctcttcggaa atcttatagc tctgtcctta gggttaacgc cgaatttcaa gtccaacttc
     1741 gacctcgccg aggacgccaa gttgcaattg tccaaggata cctacgacga tgacctcgac
     1801 aacttactgg ctcagatcgg cgaccagtac gctgacctct tcttggccgc taagaacttg
     1861 tcagacgcaa tcctcttaag tgatatctta cgtgtgaaca cagagattac gaaagctcct
     1921 ttgagcgcat ccatgatcaa gagatacgat gagcaccatc aggatttgac gttgttgaag
     1981 gccttagttc gtcagcaatt gcccgagaag tacaaggaga tcttcttcga tcagtccaag
     2041 aacggatacg cagggtacat cgatggtggt gctagtcaag aggagtttta caagttcatc
     2101 aagcccatac tcgagaagat ggatggaact gaggaactgt tagtgaagct gaatcgtgag
     2161 gacctgttaa ggaagcagcg aacgttcgac aatggcagca tcccgcacca gatccacctt
     2221 ggcgagctgc atgcaatctt gaggagacag gaagacttct atccattctt gaaggacaac
     2281 agagagaaga tcgagaagat cttgacgttc agaatcccgt attacgttgg tccactcgcc
     2341 aggggtaact cccgtttcgc ttggatgacg aggaaatcag aggagacaat cactccttgg
     2401 aacttcgagg aggtggttga caaaggagcc agcgctcaat ccttcataga gcgtatgacc
     2461 aatttcgaca agaatctccc aaacgagaag gtcctgccga aacactcatt gttgtacgag
     2521 tacttcactg tgtataacga gctcacgaag gtcaaatacg taactgaggg tatgcgaaag
     2581 cccgccttcc tctccggtga acagaagaag gcaatcgtcg atttgttgtt caagaccaac
     2641 aggaaggtca ctgtgaagca actgaaagag gactacttca agaagataga gtgcttcgat
     2701 tctgtggaga tcagtggtgt tgaggatcgt ttcaacgcat ccttgggcac ataccatgat
     2761 ttgttgaaga tcatcaagga caaggacttc ctcgacaacg aggagaacga ggacatcctc
     2821 gaggatatcg ttctcacatt gacgctcttc gaggatcgtg agatgatcga ggagagactg
     2881 aagacctacg ctcatttgtt cgatgataag gtgatgaagc aactcaagag gcgtcgatac
     2941 acgggatggg gaaggttgtc tcggaagctg atcaacggta ttagggataa acagtctggc
     3001 aagacaatct tggacttctt gaagagtgat ggattcgcaa ataggaattt catgcagtta
     3061 atccacgatg atagtctgac gttcaaggag gacatccaaa aggcccaagt gagtggtcag
     3121 ggagacagcc tccatgaaca tatagcaaac ttggctggat ccccagcaat taagaaagga
     3181 atcttgcaga cggtcaaagt tgtggatgaa cttgttaagg tcatgggtcg tcacaaacct
     3241 gagaatatcg ttatcgaaat ggcccgagag aatcagacga cccagaaagg acagaagaac
     3301 tctcgtgaac gtatgaaaca aatagaagaa gggatcaaag agctcggtag ccagatcctg
     3361 aaggagcacc cagtagagaa cactcaactg cagaacgaga agctgtatct gtactacttg
     3421 caaaacgggc gagatatgta tgttgaccag gagctcgaca tcaatagact ttctgactac
     3481 gatgttgacc atattgtgcc gcaaagcttc ttgaaggatg atagcataga taacaaagtt
     3541 ctcacacgtt ccgacaagaa cagaggtaag agcgacaacg tgccgagtga agaagtcgtt
     3601 aagaagatga agaactactg gaggcagttg ctcaacgcta agttgatcac ccagaggaag
     3661 ttcgacaacc tcacaaaggc tgagagagga ggcctgtcag agttggataa ggctgggttc
     3721 atcaagaggc agcttgtcga gacacgacag atcaccaagc acgtagccca gatcctcgat
     3781 tcaaggatga acaccaagta cgatgagaac gacaagctca tcagggaagt gaaggtaatc
     3841 acgctgaaga gcaagctcgt atccgacttc cgaaaggact ttcagttcta caaggtgcgt
     3901 gagatcaaca actaccacca tgctcatgat gcatatctta atgcagtggt tgggacggct
     3961 ctcattaaga agtacccaaa gctcgaatct gaatttgtgt atggcgatta caaggtttat
     4021 gatgtccgta agatgattgc taagagcgag caggagattg gtaaggctac cgcgaagtac
     4081 ttcttctact ctaacatcat gaacttcttc aagacggaga tcaccctggc caacggagag
     4141 atcaggaagc gtccgctcat cgagaccaac ggtgagaccg gtgagatcgt gtgggacaag
     4201 ggacgtgact tcgccactgt gaggaaggtg ttgagcatgc cgcaggtgaa catcgtgaag
     4261 aagacggagg tgcagaccgg agggttctcc aaggaatcaa tcttgccaaa gaggaactcc
     4321 gataaactca tagcaaggaa gaaggattgg gatcccaaga agtatggagg gttcgactcc
     4381 ccgaccgtcg cctacagcgt cttggttgtg gcaaaggtgg agaagggaaa gagcaagaag
     4441 ttgaaatcag tcaaggagct ccttggaatc acaatcatgg agcgaagttc tttcgagaag
     4501 aaccccatag acttcttgga ggccaaaggt tacaaagaag tgaagaaaga cttgataatc
     4561 aagttgccta agtactcctt gttcgagctc gagaacggga ggaagcgaat gttggccagc
     4621 gccggagaac ttcagaaagg caacgagctc gctctcccaa gcaagtacgt gaatttcttg
     4681 tacctcgcat cacactacga gaagctgaag ggcagcccgg aggataacga acagaagcag
     4741 ctcttcgtgg agcaacacaa gcactacttg gatgagatca tcgagcagat tagcgagttc
     4801 agcaagaggg taatcttggc tgacgctaat ctggacaagg ttctcagcgc atacaacaag
     4861 catcgtgaca aacccatccg agagcaggca gagaacatca tccacttgtt caccctgact
     4921 aatttgggag cacccgcagc cttcaagtac ttcgatacta ccatcgaccg gaagagatac
     4981 acaagtacca aggaggtcct ggatgcaacc ttgatccatc agtccatcac cggcttgtac
     5041 gagactagga tcgacctctc tcagttgggt ggagacaagc gaccggcagc cacgaagaaa
     5101 gcaggccagg ccaagaagaa gaagtaacgc gtcgacggct gcttgtcaca agctaaattt
     5161 gatcctattt cctacattaa tcgaattata tatatataaa ataaacaaaa aacaaacaaa
     5221 aatacaaata aacaaaaaac aacaaaaaga gtcttcggac caaagtttgg cttttcagcc
     5281 ttattttgtt tttgtttttt gtttttgatt ttttgtttgt tttttatttt tgtttttgtt
     5341 tggtttttga tttaaaaatg caaaataaac agagatttaa gcttaaataa gtggtattaa
     5401 aagtactata tttcatcaaa agataactga gcaacattat cagaaacccc ttcctcctct
     5461 tcttcaactg atctctcaag atttgagctc agttctttaa gctgtttttt aatctgcttc
     5521 tcactgtttc ctttaggaat tatcagcttg caagcctcaa tgaatgcttg agatctagct
     5581 ctaatagccc tgtttagagg tatgaccatg caacttttgt ctttatcagc tctgggtgaa
     5641 tcacaaaact cctttgtcca agaatacatg acactaccaa aagaaacccc cttcttgtat
     5701 tcttgactgc acatcaagtg cagctgacag cagttttctg gagtgttatt cattttcgga
     5761 atagaccagt tcagataaaa aacaaaacag ataggatcag ttattgtccc ctgacccttc
     5821 agaatgattt gcttttcaga tggcatgttg ggatcgacca gagcaaccac aagtttccct
     5881 gtagggtttg gtatagtggg gcagacccat atcacaatcc tggaaatcat catgtattgt
     5941 tttctgctgt cccaagttgg acagatcttg atgaccttat tagcgttttg cttcccgttg
     6001 ccgacaaaaa gatcattttt ccagtttgag atatggtggt ttgtatctac tatcattctt
     6061 gcagaaagat cataaccctc tgattctgtg atagaatcag attcatatgt tccaaaggaa
     6121 gatgttccct ctgtgttcaa cagtatcttt cctttggatg tatccatagc tttggttaaa
     6181 gcaagctttt catcagaaga agaccatggt tttgagactt caacattacc gttatgttta
     6241 gcaagtgaaa ctaaaggccc ttcatccttt ctggcagact tagaagaccc cttattacca
     6301 aaaaaagtca acattttgag ttcaacagcc tgaggtagag gagcttgtgt aatgtaattg
     6361 attgattctt agcaaagtgt ataataggta tatttctgat gcactgattg ctctgggtcg
     6421 gcatggcatc tccacctcct cgcggtccga cctgggcatc cgaaggagga cgtcgtccac
     6481 tcggatggct aagggagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     6541 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     6601 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     6661 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     6721 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcggaat tcagattgtc
     6781 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
     6841 agagaaaaga gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc
     6901 gttcgtccat ttgtatgtgc atgccaacca caggagatct cagtaaagcg aaaagtgcca
     6961 cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
     7021 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
     7081 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
     7141 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
     7201 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
     7261 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
     7321 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
     7381 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
     7441 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
     7501 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
     7561 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
     7621 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
     7681 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
     7741 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
     7801 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
     7861 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
     7921 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc
     7981 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt
     8041 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat
     8101 agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta
     8161 gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc
     8221 actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa
     8281 tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac
     8341 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga
     8401 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     8461 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa
     8521 ctatgcggca tcagagcaga ttgtactgag agtgcaccat agatcaacga cattactata
     8581 tatataatat aggaagcatt taatagaaca gcatcgtaat atatgtgtac tttgcagtta
     8641 tgacgccaga tggcagtagt ggaagatatt ctttattgaa aaatagcttg tcaccttacg
     8701 tacaatcttg atccggagct tttctttttt tgccgattaa gaattaattc ggtcgaaaaa
     8761 agaaaaggag agggccaaga gggagggcat tggtgactat tgagcacgtg agtatacgtg
     8821 attaagcaca caaaggcagc ttggagtatg tctgttatta atttcacagg tagttctggt
     8881 ccattggtga aagtttgcgg cttgcagagc acagaggccg cagaatgtgc tctagattcc
     8941 gatgctgact tgctgggtat tatatgtgtg cccaatagaa agagaacaat tgacccggtt
     9001 attgcaagga aaatttcaag tcttgtaaaa gcatataaaa atagttcagg cactccgaaa
     9061 tacttggttg gcgtgtttcg taatcaacct aaggaggatg ttttggctct ggtcaatgat
     9121 tacggcattg atatcgtcca actgcatgga gatgagtcgt ggcaagaata ccaagagttc
     9181 ctcggtttgc cagttattaa aagactcgta tttccaaaag actgcaacat actactcagt
     9241 gcagcttcac agaaacctca ttcgtttatt cccttgtttg attcagaagc aggtgggaca
     9301 ggtgaacttt tggattggaa ctcgatttct gactgggttg gaaggcaaga gagccccgaa
     9361 agcttacatt ttatgttagc tggtggactg acgccagaaa atgttggtga tgcgcttaga
     9421 ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg agacaaatgg tgtaaaagac
     9481 tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat aggttattac tgagtagtat
     9541 ttatttaagt attgtttgtg cacttgccga tctatgcggt gtgaaatacc gcacagatgc
     9601 gtaaggagaa aataccgcat caggaaattg taagcgttaa tattttgtta aaattcgcgt
     9661 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     9721 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     9781 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     9841 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     9901 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     9961 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
    10021 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
    10081 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    10141 attacgccag atccttgctg gctgaacccc cagccggaac tgaccccaca aggccctagc
    10201 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
    10261 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
    10321 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
    10381 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
    10441 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
    10501 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
    10561 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
    10621 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
    10681 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
    10741 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
    10801 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
    10861 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
    10921 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
    10981 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
    11041 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
    11101 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
    11161 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctgggccatc gagccgacgg
    11221 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
    11281 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
    11341 tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc ccttattcct
    11401 gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca atgaagtcgg
    11461 tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg ccctgcacga
    11521 ataccagcga ccccttgccc aaatacttgc cgtgggcctc ggcctgagag ccaaaacact
    11581 tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc cacatctagg
    11641 tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc aggcttgatc
    11701 cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc cctgatcgac
    11761 cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc aagatcaata
    11821 aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa
    11881 aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc gcgcggatct
    11941 ttaaatgggg tgtcttcttc ccagttttcg caatccacat cggccagatc gttattcagt
    12001 aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca atccgatatg
    12061 tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt ttcagggctt
    12121 tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat gagcagattg
    12181 ctccagccat catgccgttc aaggtgcagg acctttggaa caggcagctt tccttccagc
    12241 catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata ccggctgtcc
    12301 gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt agcaggagac
    12361 attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa ttccggtgat
    12421 attctcattt tagccattta ttatttcctt cctcttttct acagtattta aagatacccc
    12481 aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc taaaacttta
    12541 aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac atagtatcga
    12601 cggagccgat tttgaaacca caattatggg tgatgctgca actcgagcgg gccgggaggg
    12661 ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca cgcgcacagg gcgcagccct
    12721 ggttaaaaac aaggtttata aatattggtt taaaagcagg ttaaaagaca ggttagcggt
    12781 ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg gacagcccct
    12841 caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt gtcaaggatc
    12901 gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca ataccgcagg gcacttatcc
    12961 ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa tcaggcgttt tcgccgattt
    13021 gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc ctgcccctca tctgtcaacg
    13081 ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc ccctcatctg tcagtgaggg
    13141 ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg gtgtctcgca cacggcttcg
    13201 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
    13261 ccagcccggt gagcgtctct agtggactga tgggctgcct gtatcgagtg gtgattttgt
    13321 gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt gtggtgtaaa
    13381 caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt actggggtgg
    13441 tttt
//
