LOCUS       V004902                 9914 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004902
VERSION     V004902
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9914)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ,
            Atsma DE.
  TITLE     Fibroblasts from human postmyocardial infarction scars acquire
            properties of cardiomyocytes after transduction with a recombinant
            myocardin gene
  JOURNAL   FASEB J. 21 (12), 3369-3379 (2007)
   PUBMED   17579192
REFERENCE   2  (bases 1 to 9914)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300
            RC, Netherlands
REFERENCE   3  (bases 1 to 9914)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9914)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FASEB J.";
            date: "2007"; volume: "21"; issue: "12"; pages: "3369-3379"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-DEC-2006) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC,
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     repeat_region   212..410
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1939..2242
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2243..2446
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             5632..5658
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    5701..6289
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6428..6661
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6733..6867
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6894..7029
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7050..7068)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7078..7094)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      7239..7694
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7723..7827
                     /label="AmpR promoter"
     CDS             7828..8685
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8859..9447
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9735..9756
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9772..9802
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9810..9826
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9834..9850
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9871..9889
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1921 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1981 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2041 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2101 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2161 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2221 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2281 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2341 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2401 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2461 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     2521 gctagaggat cccccgggct gcaggaattc ggcgcgccac gcgtaccgat atcatcgtcg
     2581 acgccgtata cgctccggga gcctgttgct ggtggagaac agggggcgcc tggccaaggg
     2641 accagcggct tgctgagact caacatgaca ctcctggggt ctgagcattc cttgctgatt
     2701 aggagcaagt tcagatcagt tttacagtta agacttcaac aaagaaggac ccaggaacaa
     2761 ctggctaacc aaggcataat accaccactg aaacgtccag ctgaattcca tgagcaaaga
     2821 aaacatttgg atagtgacaa ggctaaaaat tccctgaagc gcaaagccag aaacaggtgc
     2881 aacagtgccg acttggttaa tatgcacata ctccaagctt ccactgcgga gaggtccatt
     2941 ccaactgctc agatgaagct gaaaagagcc cgactcgccg atgatctcaa tgaaaaaatt
     3001 gctctacgac cagggccact ggagctggtg gaaaaaaaca ttcttcctgt ggattctgct
     3061 gtgaaagagg ccataaaagg taaccaggtg agtttctcca aatccacgga tgcttttgcc
     3121 tttgaagagg acagcagcag cgatgggctt tctccggatc agactcgaag tgaagacccc
     3181 caaaactcag cgggatcccc gccagacgct aaagcctcag ataccccttc gacaggttct
     3241 ctggggacaa accaggatct tgcttctggc tcagaaaatg acagaaatga ctcagcctca
     3301 cagcccagcc accagtcaga tgcggggaag caggggcttg gcccccccag cacccccata
     3361 gccgtgcatg ctgctgtaaa gtccaaatcc ttgggtgaca gtaagaaccg ccacaaaaag
     3421 cccaaggacc ccaagccaaa ggtgaagaag cttaaatatc accagtacat tcccccagac
     3481 cagaaggcag agaagtcccc tccacctatg gactcagcct acgctcggct gctccagcaa
     3541 cagcagctgt tcctgcagct ccaaatcctc agccagcagc agcagcagca gcaacaccga
     3601 ttcagctacc tagggatgca ccaagctcag cttaaggaac caaatgaaca gatggtcaga
     3661 aatccaaact cttcttcaac gccactgagc aataccccct tgtctcctgt caaaaacagt
     3721 ttttctggac aaactggtgt ctcttctttc aaaccaggcc cactcccacc taacctggat
     3781 gatctgaagg tctctgaatt aagacaacag cttcgaattc ggggcttgcc tgtgtcaggc
     3841 accaaaacgg ctctcatgga ccggcttcga cccttccagg actgctctgg caacccagtg
     3901 ccgaactttg gggatataac gactgtcact tttcctgtca cacccaacac gctgcccaat
     3961 taccagtctt cctcttctac cagtgccctg tccaacggct tctaccactt tggcagcacc
     4021 agctccagcc ccccgatctc cccagcctcc tctgacctgt cagtcgctgg gtccctgccg
     4081 gacaccttca atgatgcctc cccctccttc ggcctgcacc cgtccccagt ccacgtgtgc
     4141 acggaggaaa gtctcatgag cagcctgaat gggggctctg ttccttctga gctggatggg
     4201 ctggactccg agaaggacaa gatgctggtg gagaagcaga aggtgatcaa tgaactcacc
     4261 tggaaactcc agcaagagca gaggcaggtg gaggagctga ggatgcagct tcagaagcag
     4321 aaaaggaata actgttcaga gaagaagccg ctgcctttcc tggctgcctc catcaagcag
     4381 gaagaggctg tctccagctg tccttttgca tcccaagtac ctgtgaaaag acaaagcagc
     4441 agctcagagt gtcacccacc ggcttgtgaa gctgctcaac tccagcctct tggaaatgct
     4501 cattgtgtgg agtcctcaga tcaaaccaat gtactttctt ccacatttct cagcccccag
     4561 tgttcccctc agcattcacc gctgggggct gtgaaaagcc cacagcacat cagtttgccc
     4621 ccatcaccca acaaccctca ctttctgccc tcatcctccg gggcccaggg agaagggcac
     4681 agggtctcct cgcccatcag cagccaggtg tgcactgcac agaactcagg agcacacgat
     4741 ggccatcctc caagcttctc tccccattct tccagcctcc acccgccctt ctctggagcc
     4801 caagcagaca gcagtcatgg tgccggggga aacccttgtc ccaaaagccc atgtgtacag
     4861 caaaagatgg ctggtttaca ctcttctgat aaggtggggc caaagttttc aattccatcc
     4921 ccaacttttt ctaagtcaag ttcagcaatt tcagaggtaa cacagcctcc atcctatgaa
     4981 gatgccgtaa agcagcaaat gacccggagt cagcagatgg atgaactcct ggacgtgctt
     5041 attgaaagcg gagaaatgcc agcagacgct agagaggatc actcatgtct tcaaaaagtc
     5101 ccaaagatac ccagatcttc ccgaagtcca actgctgtcc tcaccaagcc ctcggcttcc
     5161 tttgaacaag cctcttcagg cagccagatc ccctttgatc cctatgccac cgacagtgat
     5221 gagcatcttg aagtcttatt aaattcccag agccccctag gaaagatgag tgatgtcacc
     5281 cttctaaaaa ttgggagcga agagcctcac tttgatggga taatggatgg attctctggg
     5341 aaggctgcag aagacctctt caatgcacat gagatcttgc caggccccct ctctccaatg
     5401 cagacacagt tttcaccctc ttctgtggac agcaatgggc tgcagttaag cttcactgaa
     5461 tctccctggg aaaccatgga gtggctggac ctcactccgc caaattccac accaggcttt
     5521 agcgccctca ccaccagcag ccccagcatc ttcaacatcg atttcctgga tgtcactgat
     5581 ctcaatttga attcttccat ggaccttcac ttgcagcagt ggaccggtgg ctacccatac
     5641 gatgttccag attacgctct ttaaatgtac agcagactct agaggtaccg gttgttcgat
     5701 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     5761 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5821 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5881 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5941 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     6001 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     6061 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     6121 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     6181 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     6241 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgatcgaacg
     6301 ttagccggct acatggcgca atcactagag cggccgccac cgcggaattc gagctcggta
     6361 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6421 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6661 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6721 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     6781 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     6841 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     6901 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6961 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     7021 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     7081 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     7141 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     7201 ttcccaacag ttgcgcagcc tgaatggcga atggcgcgac gcgccctgta gcggcgcatt
     7261 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7321 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7381 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7441 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7501 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7561 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7621 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     7681 aacgtttaca atttcccagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     7741 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7801 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7861 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7921 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7981 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8041 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8101 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8161 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8221 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8281 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8341 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8401 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8461 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     8521 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     8581 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     8641 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     8701 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     8761 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8821 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8881 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8941 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9001 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9061 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9121 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9181 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9241 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9301 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9361 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     9421 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     9481 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     9541 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     9601 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     9661 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     9721 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggg ctttacactt
     9781 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     9841 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
     9901 ggagctgcaa gctt
//
