LOCUS       V004903                 9943 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004903
VERSION     V004903
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9943)
  AUTHORS   van Tuyn J, Sluiter JP, Swildens J, Goumans M-J., Doevendans PA, van
            der Laarse A, Schalij MJ, Atsma DE, de Vries AA.
  TITLE     Gene expression profiling of myocardin-transduced human cardiac
            progenitor cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9943)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUN-2007) MCB, LUMC, Einthovenweg 20, Leiden, ZH
            2333ZH, the Netherlands
REFERENCE   3  (bases 1 to 9943)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9943)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-JUN-2007) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2333ZH, the
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     repeat_region   212..410
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1799..1916
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1939..2242
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2243..2446
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2650..2775
                     /codon_start=1
                     /gene="MYOCD"
                     /product="truncated cardiac myocardin"
                     /label="MYOCD"
                     /note="N-terminal region"
                     /protein_id="ABS20111.1"
                     /translation="MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPH"
     CDS             5661..5687
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    5730..6318
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6457..6690
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6762..6896
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6923..7058
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(7079..7097)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7107..7123)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      7268..7723
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7752..7856
                     /label="AmpR promoter"
     CDS             7857..8714
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8888..9476
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9764..9785
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9801..9831
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9839..9855
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9863..9879
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9900..9918
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     1861 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg
     1921 ataagcttgg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     1981 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2041 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2101 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2161 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2221 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2281 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2341 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2401 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
     2461 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta
     2521 gctagaggat cccccgggct gcaggaattc tgggttgtta gctgcggtca gctgggctcc
     2581 cgggagcctg ttgctggtgg agaacagggg gcacctggcc aagggaccag cggcttgctg
     2641 agactcaaca tgacactcct ggggtctgag cattccttgc tgattaggag caagttcaga
     2701 tcagttttac agttaagact tcaacaaaga aggacccagg aacaactggc taaccaaggc
     2761 ataataccac actaatccaa gctacagcag ccacatactt actgcaaaac cttgcactga
     2821 aacgtccagc tgaattccat gagcaaagaa aacatttgga tagtgacaag gctaaaaatt
     2881 ccctgaagcg caaagccaga aacaggtgca acagtgccga cttggttaat atgcacatac
     2941 tccaagcttc cactgcggag aggtccattc caactgctca gatgaagctg aaaagagccc
     3001 gactcgccga tgatctcaat gaaaaaattg ctctacgacc agggccactg gagctggtgg
     3061 aaaaaaacat tcttcctgtg gattctgctg tgaaagaggc cataaaaggt aaccaggtga
     3121 gtttctccaa atccacggat gcttttgcct ttgaagagga cagcagcagc gatgggcttt
     3181 ctccggatca gactcgaagt gaagaccccc aaaactcagc gggatccccg ccagacgcta
     3241 aagcctcaga taccccttcg acaggttctc tggggacaaa ccaggatctt gcttctggct
     3301 cagaaaatga cagaaatgac tcagcctcac agcccagcca ccagtcagat gcggggaagc
     3361 aggggcttgg cccccccagc acccccatag ccgtgcatgc tgctgtaaag tccaaatcct
     3421 tgggtgacag taagaaccgc cacaaaaagc ccaaggaccc caagccaaag gtgaagaagc
     3481 ttaaatatca ccagtacatt cccccagacc agaaggcaga gaagtcccct ccacctatgg
     3541 actcagccta cgctcggctg ctccagcaac agcagctgtt cctgcagctc caaatcctca
     3601 gccagcagca gcagcagcag caacaccgat tcagctacct agggatgcac caagctcagc
     3661 ttaaggaacc aaatgaacag atggtcagaa atccaaactc ttcttcaacg ccactgagca
     3721 ataccccctt gtctcctgtc aaaaacagtt tttctggaca aactggtgtc tcttctttca
     3781 aaccaggccc actcccacct aacctggatg atctgaaggt ctctgaatta agacaacagc
     3841 ttcgaattcg gggcttgcct gtgtcaggca ccaaaacggc tctcatggac cggcttcgac
     3901 ccttccagga ctgctctggc aacccagtgc cgaactttgg ggatataacg actgtcactt
     3961 ttcctgtcac acccaacacg ctgcccaatt accagtcttc ctcttctacc agtgccctgt
     4021 ccaacggctt ctaccacttt ggcagcacca gctccagccc cccgatctcc ccagcctcct
     4081 ctgacctgtc agtcgctggg tccctgccgg acaccttcaa tgatgcctcc ccctccttcg
     4141 gcctgcaccc gtccccagtc cacgtgtgca cggaggaaag tctcatgagc agcctgaatg
     4201 ggggctctgt tccttctgag ctggatgggc tggactccga gaaggacaag atgctggtgg
     4261 agaagcagaa ggtgatcaat gaactcacct ggaaactcca gcaagagcag aggcaggtgg
     4321 aggagctgag gatgcagctt cagaagcaga aaaggaataa ctgttcagag aagaagccgc
     4381 tgcctttcct ggctgcctcc atcaagcagg aagaggctgt ctccagctgt ccttttgcat
     4441 cccaagtacc tgtgaaaaga caaagcagca gctcagagtg tcacccaccg gcttgtgaag
     4501 ctgctcaact ccagcctctt ggaaatgctc attgtgtgga gtcctcagat caaaccaatg
     4561 tactttcttc cacatttctc agcccccagt gttcccctca gcattcaccg ctgggggctg
     4621 tgaaaagccc acagcacatc agtttgcccc catcacccaa caaccctcac tttctgccct
     4681 catcctccgg ggcccaggga gaagggcaca gggtctcctc gcccatcagc agccaggtgt
     4741 gcactgcaca gaactcagga gcacacgatg gccatcctcc aagcttctct ccccattctt
     4801 ccagcctcca cccgcccttc tctggagccc aagcagacag cagtcatggt gccgggggaa
     4861 acccttgtcc caaaagccca tgtgtacagc aaaagatggc tggtttacac tcttctgata
     4921 aggtggggcc aaagttttca attccatccc caactttttc taagtcaagt tcagcaattt
     4981 cagaggtaac acagcctcca tcctatgaag atgccgtaaa gcagcaaatg acccggagtc
     5041 agcagatgga tgaactcctg gacgtgctta ttgaaagcgg agaaatgcca gcagacgcta
     5101 gagaggatca ctcatgtctt caaaaagtcc caaagatacc cagatcttcc cgaagtccaa
     5161 ctgctgtcct caccaagccc tcggcttcct ttgaacaagc ctcttcaggc agccagatcc
     5221 cctttgatcc ctatgccacc gacagtgatg agcatcttga agtcttatta aattcccaga
     5281 gccccctagg aaagatgagt gatgtcaccc ttctaaaaat tgggagcgaa gagcctcact
     5341 ttgatgggat aatggatgga ttctctggga aggctgcaga agacctcttc aatgcacatg
     5401 agatcttgcc aggccccctc tctccaatgc agacacagtt ttcaccctct tctgtggaca
     5461 gcaatgggct gcagttaagc ttcactgaat ctccctggga aaccatggag tggctggacc
     5521 tcactccgcc aaattccaca ccaggcttta gcgccctcac caccagcagc cccagcatct
     5581 tcaacatcga tttcctggat gtcactgatc tcaatttgaa ttcttccatg gaccttcact
     5641 tgcagcagtg gaccggtggc tacccatacg atgttccaga ttacgctctt taaatgtaca
     5701 gcagactcta gaggtaccgg ttgttcgata atcaacctct ggattacaaa atttgtgaaa
     5761 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     5821 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     5881 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     5941 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6001 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     6061 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     6121 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     6181 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     6241 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     6301 tttgggccgc ctccccgcct gatcgaacgt tagccggcta catggcgcaa tcactagagc
     6361 ggccgccacc gcggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     6421 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     6481 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     6541 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6601 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6661 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     6721 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6781 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6841 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6901 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     7021 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     7081 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7141 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7201 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7261 tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     7321 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     7381 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     7441 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     7501 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     7561 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     7621 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     7681 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccaggt ggcacttttc
     7741 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     7801 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     7861 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     7921 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     7981 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     8041 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     8101 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     8161 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     8221 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     8281 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     8341 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     8401 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     8461 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     8521 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     8581 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     8641 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     8701 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     8761 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     8821 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     8881 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     8941 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     9001 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     9061 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     9121 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     9181 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     9241 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     9301 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     9361 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     9421 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     9481 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     9541 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     9601 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     9661 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     9721 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     9781 ctcattaggc accccagggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     9841 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca
     9901 attaaccctc actaaaggga acaaaagctg gagctgcaag ctt
//
