LOCUS       V010144                 8629 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V010144
VERSION     V010144
KEYWORDS    pLgw V5-EcoDam
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8629)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W,
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
   PUBMED   17038565
REFERENCE   2  (bases 1 to 8629)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8629)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome
            Res."; date: "2006-12"; volume: "16(12)"; pages: "1493-504. Epub
            2006 Oct 12"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      36..171
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        233..280
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             299..670
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    803..936
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(973..989)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(997..1013)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1021..1051)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1066..1087)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(1204..1221)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1375..1963)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2137..2994)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2995..3099)
                     /label="AmpR promoter"
     primer_bind     complement(3174..3193)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     enhancer        3365..3744
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3745..3947
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             3962..4142
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4186..4311
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    4804..5037
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             5222..5266
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             5415..5456
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        5728..5966
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    6019..6043
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6119..6143)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6154..6195
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     CDS             6214..7047
                     /gene="dam"
                     /label="DNA adenine methylase"
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     LTR             7560..7740
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    7772..7996
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8042..8470
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        8484..8629
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
       61 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
      121 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
      181 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
      241 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
      301 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
      361 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
      421 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
      481 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
      541 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
      601 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
      661 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
      721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
      781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
      841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
      961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1561 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1801 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3121 acctgacgtc gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc
     3181 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     3241 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     3301 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     3361 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     3421 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     3481 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     3541 gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta
     3601 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3661 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3721 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     3781 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     3841 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     3901 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac
     3961 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     4021 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     4081 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     4141 cagtggcgcc cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg
     4201 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     4261 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     4321 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     4381 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     4441 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     4501 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     4561 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     4621 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     4681 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     4741 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     4801 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcctc
     4861 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     4921 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     4981 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     5041 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     5101 gagtaataaa tctctggaac agatctggaa tcacacgacc tggatggagt gggacagaga
     5161 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     5221 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     5281 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     5341 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     5401 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     5461 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcccccgg
     5521 gctgcaggaa ttcagatctc ggccgcatat taagtgcatt gttctcgata ccgctaagtg
     5581 cattgttctc gttagctcga tggacaagtg cattgttctc ttgctgaaag ctcgatggac
     5641 aagtgcattg ttctcttgct gaaagctcga tggacaagtg cattgttctc ttgctgaaag
     5701 ctcagtaccc gggagtaccc tcgaccgccg gagtataaat agaggcgctt cgtctacgga
     5761 gcgacaattc aattcaaaca agcaaagtga acacgtcgct aagcgaaagc taagcaaata
     5821 aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa
     5881 ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa
     5941 ttattgaata caagaagaga actctgaata ctttcaacaa gttaccgaga aagaagaact
     6001 cacacacagc tagatcaaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
     6061 accatggaaa agaatggaac tgatgaagta tatattttta ataagggtgg gcgcgccgac
     6121 ccagctttct tgtacaaagt ggttcgatcc ggtggtaagc ctatccctaa ccctctcctc
     6181 ggtctcgatt ctacgatcga tttcgaaccg cggatgaaga aaaatcgcgc ttttttgaag
     6241 tgggcagggg gcaagtatcc cctgcttgat gatattaaac ggcatttgcc caagggcgaa
     6301 tgtctggttg agccttttgt aggtgccggg tcggtgtttc tcaacaccga cttttctcgt
     6361 tatatccttg ccgatatcaa tagcgacctg atcagtctct ataacattgt gaagatgcgt
     6421 actgatgagt acgtacaggc cgcacgcgag ctgtttgttc ccgaaacaaa ttgcgccgag
     6481 gtttactatc agttccgcga agagttcaac aaaagccagg atccgttccg tcgggcggta
     6541 ctgtttttat atttgaaccg ctacggttac aacggcctgt gtcgttacaa tctgcgcggt
     6601 gagtttaacg tgccgttcgg ccgctacaaa aaaccctatt tcccggaagc agagttgtat
     6661 cacttcgctg aaaaagcgca gaatgccttt ttctattgtg agtcttacgc cgatagcatg
     6721 gcgcgcgcag atgatgcatc cgtcgtctat tgcgatccgc cttatgcacc gctgtctgcg
     6781 accgccaact ttacggcgta tcacacaaac agttttacgc ttgaacaaca agcgcatctg
     6841 gcggagatcg ccgaaggtct ggttgagcgc catattccag tgctgatctc caatcacgat
     6901 acgatgttaa cgcgtgagtg gtatcagcgc gcaaaattgc atgtcgtcaa agttcgacgc
     6961 agtataagca gcaacggcgg cacacgtaaa aaggtggacg aactgctggc tttgtacaaa
     7021 ccaggagtcg tttcacccgc gaaaaaataa ctcgagacct agaaaaacat ggagcaatca
     7081 caagtagcaa tacagcagct accaatgctg cttgtgcctg gctagaagca caagaggagg
     7141 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7201 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7261 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7321 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7381 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7441 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7501 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7561 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7621 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7681 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7741 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7801 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7861 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7921 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7981 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8041 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8101 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8161 acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg gttccgattt
     8221 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8281 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8341 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8401 taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8461 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8521 caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     8581 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatt
//
