LOCUS       V005021                 7683 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005021
VERSION     V005021
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
  TITLE     Generalized extracellular molecule sensor platform for programming
            cellular behavior
  JOURNAL   Nat. Chem. Biol. 14 (7), 723-729 (2018)
   PUBMED   29686358
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Scheller L, Strittmatter T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
            4058, Switzerland
REFERENCE   3  (bases 1 to 7683)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7683)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
            Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..215
                     /label="Psv40"
                     /note="Psv40"
     promoter        18..214
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      65..200
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     regulatory      240..249
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             273..3002
                     /gene="IL6ST"
                     /label="Interleukin-6 receptor subunit beta"
                     /note="Interleukin-6 receptor subunit beta from Homo
                     sapiens. Accession#: P40189"
     polyA_signal    3056..3280
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3326..3754
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3768..4097
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             4164..4955
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5132..5265
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5302..5318)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(5326..5342)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5350..5380)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5395..5416)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5704..6289)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6463..7320)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7321..7425)
                     /label="AmpR promoter"
ORIGIN
        1 acgcgtgatc tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
       61 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat
      121 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      181 aggaggcttt tttggaggcc taggcttttg caaaaagctt cgaatcgcgc tagcggccgg
      241 ccaccatgga aactgatact ttgctgctct gggtcctgct gctgtgggtc cctggatcaa
      301 cgggggacgg atccgaactt ctagatccat gtggttatat cagtcctgaa tctccagttg
      361 tacaacttca ttctaatttc actgcagttt gtgtgctaaa ggaaaaatgt atggattatt
      421 ttcatgtaaa tgctaattac attgtctgga aaacaaacca ttttactatt cctaaggagc
      481 aatatactat cataaacaga acagcatcca gtgtcacctt tacagatata gcttcattaa
      541 atattcagct cacttgcaac attcttacat tcggacagct tgaacagaat gtttatggaa
      601 tcacaataat ttcaggcttg cctccagaaa aacctaaaaa tttgagttgc attgtgaacg
      661 aggggaagaa aatgaggtgt gagtgggatc gtggaaggga aacacacttg gagacaaact
      721 tcactttaaa atctgaatgg gcaacacaca agtttgctga ttgcaaagca aaacgtgaca
      781 cccccacctc atgcactgtt gattattcta ctgtgtattt tgtcaacatt gaagtctggg
      841 tagaagcaga gaatgccctt gggaaggtta catcagatca tatcaatttt gatcctgtat
      901 ataaagtgaa gcccaatccg ccacataatt tatcagtgat caactcagag gaactgtcta
      961 gtatcttaaa attgacatgg accaacccaa gtattaagag tgttataata ctaaaatata
     1021 acattcaata taggaccaaa gatgcctcaa cttggagcca gattcctcct gaagacacag
     1081 catccacccg atcttcattc actgtccaag accttaaacc ttttacagaa tatgtgttta
     1141 ggattcgctg tatgaaggaa gatggtaagg gatactggag tgactggagt gaagaagcaa
     1201 gtgggatcac ctatgaagat agaccatcta aagcaccaag tttctggtat aaaatagatc
     1261 catcccatac tcaaggctac agaactgtac aactcgtgtg gaagacattg cctccttttg
     1321 aagccaatgg aaaaatcttg gattatgaag tgactctcac aagatggaaa tcacatttac
     1381 aaaattacac agttaatgcc acaaaactga cagtaaatct cacaaatgat cgctatgtag
     1441 caaccctaac agtaagaaat cttgttggca aatcagatgc agctgtttta actatccctg
     1501 cctgtgactt tcaagctact caccctgtaa tggatcttaa agcattcccc aaagataaca
     1561 tgctttgggt ggaatggact actccaaggg aatctgtaaa gaaatatata cttgagtggt
     1621 gtgtgttatc agataaagca ccctgtatca cagactggca acaagaagat ggtaccgtgc
     1681 atcgcaccta tttaagaggg aacttagcag agagcaaatg ctatttgata acagttactc
     1741 cagtatatgc tgatggacca ggaagccctg aatccataaa ggcatacctt aaacaagctc
     1801 caccttccaa aggacctact gttcggacaa aaaaagtagg gaaaaacgaa gctgtcttag
     1861 agtgggacca acttcctgtt gatgttcaga atggatttat cagaaattat actatatttt
     1921 atagaaccat cattggaaat gaaactgctg tgaatgtgga ttcttcccac acagaatata
     1981 cattgtcctc tttgactagt gacacattgt acatggtacg aatggcagca tacacagatg
     2041 aaggtgggaa ggatggtcca gaattcactt ttactacccc aaagtttgct caaggagaaa
     2101 ttgaagccat agtcgtgcct gtttgcttag cattcctatt gacaactctt ctgggagtgc
     2161 tgttctgctt taataagcga gacctaatta aaaaacacat ctggcctaat gttccagatc
     2221 cttcaaagag tcatattgcc cagtggtcac ctcacactcc tccaaggcac aattttaatt
     2281 caaaagatca aatgtattca gatggcaatt tcactgatgt aagtgttgtg gaaatagaag
     2341 caaatgacaa aaagcctttt ccagaagatc tgaaatcatt ggacctgttc aaaaaggaaa
     2401 aaattaatac tgaaggacac agcagtggta ttggggggtc ttcatgcatg tcatcttcta
     2461 ggccaagcat ttctagcagt gatgaaaatg aatcttcaca aaacacttcg agcactgtcc
     2521 agtattctac cgtggtacac agtggctaca gacaccaagt tccgtcagtc caagtcttct
     2581 caagatccga gtctacccag cccttgttag attcagagga gcggccagaa gatctacaat
     2641 tagtagatca tgtagatggc ggtgatggta ttttgcccag gcaacagtac ttcaaacaga
     2701 actgcagtca gcatgaatcc agtccagata tttcacattt tgaaaggtca aagcaagttt
     2761 catcagtcaa tgaggaagat tttgttagac ttaaacagca gatttcagat catatttcac
     2821 aatcctgtgg atctgggcaa atgaaaatgt ttcaggaagt ttctgcagca gatgcttttg
     2881 gtccaggtac tgagggacaa gtagaaagat ttgaaacagt tggcatggag gctgcgactg
     2941 atgaaggcat gcctaaaagt tacttaccac agactgtacg gcaaggcggc tacatgcctc
     3001 agtaggcggc cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     3061 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     3121 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     3181 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     3241 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     3301 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3361 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3421 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3481 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     3541 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     3601 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     3661 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     3721 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     3781 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3841 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     3901 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     3961 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     4021 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     4081 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     4141 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     4201 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     4261 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     4321 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     4381 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     4441 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     4501 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     4561 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     4621 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     4681 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     4741 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     4801 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     4861 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     4921 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     4981 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     5041 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     5101 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     5161 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     5221 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     5281 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5341 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5401 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     5461 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     5521 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     5581 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     5641 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     5701 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     5761 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     5821 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     5881 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     5941 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6001 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6061 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6121 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     6181 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6421 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     6481 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     6541 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     6601 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     6661 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     6721 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     6781 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     6841 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     6901 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6961 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7021 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7081 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7141 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7201 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7261 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7321 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7381 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7441 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     7501 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     7561 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     7621 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     7681 tat
//
