LOCUS       V005022                 6354 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005022
VERSION     V005022
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
  TITLE     Generalized extracellular molecule sensor platform for programming
            cellular behavior
  JOURNAL   Nat. Chem. Biol. 14 (7), 723-729 (2018)
   PUBMED   29686358
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Scheller L, Strittmatter T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel
            4058, Switzerland
REFERENCE   3  (bases 1 to 6354)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6354)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058,
            Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..215
                     /label="Psv40"
                     /note="Psv40"
     promoter        18..214
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      65..200
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     sig_peptide     246..308
                     /label="Ig-kappa leader"
                     /note="leader sequence from mouse immunoglobulin kappa
                     light chain"
     CDS             312..1673
                     /gene="IL6R"
                     /label="Interleukin-6 receptor subunit alpha"
                     /note="Interleukin-6 receptor subunit alpha from Homo
                     sapiens. Accession#: P08887"
     polyA_signal    1727..1951
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1997..2425
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2439..2768
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2835..3626
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3803..3936
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3973..3989)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3997..4013)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4021..4051)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4066..4087)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4375..4960)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5134..5991)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5992..6096)
                     /label="AmpR promoter"
ORIGIN
        1 acgcgtgatc tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
       61 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat
      121 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      181 aggaggcttt tttggaggcc taggcttttg caaaaagctt cgaatcgcgc tagcggccgg
      241 ccaccatgga aactgatact ttgctgctct gggtcctgct gctgtgggtc cctggatcaa
      301 cgggggacgg atccccggga gcggcgctgg ccccaaggcg ctgccctgcg caggaggtgg
      361 cgagaggcgt gctgaccagt ctgccaggag acagcgtgac tctgacctgc ccgggggtag
      421 agccggaaga caatgccact gttcactggg tgctcaggaa gccggctgca ggctcccacc
      481 ccagcagatg ggctggcatg ggaaggaggc tgctgctgag gtcggtgcag ctccacgact
      541 ctggaaacta ttcatgctac cgggccggcc gcccagctgg gactgtgcac ttgctggtgg
      601 atgttccccc cgaggagccc cagctctcct gcttccggaa gagccccctc agcaatgttg
      661 tttgtgagtg gggtcctcgg agcaccccat ccctgacgac aaaggctgtg ctcttggtga
      721 ggaagtttca gaacagtccg gccgaagact tccaggagcc gtgccagtat tcccaggagt
      781 cccagaagtt ctcctgccag ttagcagtcc cggagggaga cagctctttc tacatagtgt
      841 ccatgtgcgt cgccagtagt gtcgggagca agttcagcaa aactcaaacc tttcagggtt
      901 gtggaatctt gcagcctgat ccgcctgcca acatcacagt cactgccgtg gccagaaacc
      961 cccgctggct cagtgtcacc tggcaagacc cccactcctg gaactcatct ttctacagac
     1021 tacggtttga gctcagatat cgggctgaac ggtcaaagac attcacaaca tggatggtca
     1081 aggacctcca gcatcactgt gtcatccacg acgcctggag cggcctgagg cacgtggtgc
     1141 agcttcgtgc ccaggaggag ttcgggcaag gcgagtggag cgagtggagc ccggaggcca
     1201 tgggcacgcc ttggacagaa tccaggagtc ctccagctga gaacgaggtg tccaccccca
     1261 tgcaggcact tactactaat aaagacgatg ataatattct cttcagagat tctgcaaatg
     1321 cgacaagcct cccagtgcaa gattcttctt cagtaccact gcccacattc ctggttgctg
     1381 gagggagcct ggccttcgga acgctcctct gcattgccat tattctgagg ttcaagaaga
     1441 cgtggaagct gcgggctctg aaggaaggca agacaagcat gcatccgccg tactctttgg
     1501 ggcagctggt cccggagagg cctcgaccca ccccagtgct tgttcctctc atctccccac
     1561 cggtgtcccc cagcagcctg gggtctgaca atacctcgag ccacaaccga ccagatgcca
     1621 gggacccacg gagcccttat gacatcagca atacagacta cttcttcccc agataggcgg
     1681 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     1741 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     1801 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     1861 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     1921 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     1981 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     2041 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     2101 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     2161 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2221 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     2281 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2341 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2401 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa
     2461 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2521 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2581 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2641 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2701 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2761 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat
     2821 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     2881 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     2941 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3001 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3061 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     3121 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     3181 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     3241 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     3301 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     3361 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     3421 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     3481 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     3541 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     3601 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     3661 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     3721 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     3781 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
     3841 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3901 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
     3961 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4021 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4081 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4141 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4201 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4261 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4321 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4381 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4441 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4501 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4561 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4621 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4681 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4741 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     4801 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     4861 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt
     4921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     5821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     5881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     5941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6121 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     6181 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     6241 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     6301 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atat
//
