LOCUS       40924_28112        7782 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pLeo670, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Scheller L, Strittmatter T, Fuchs D, Bojar D, Fussenegger M.
  TITLE     Generalized extracellular molecule sensor platform for programming 
            cellular behavior
  JOURNAL   Nat. Chem. Biol. 14 (7), 723-729 (2018)
  PUBMED    29686358
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Scheller L, Strittmatter T.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 
            4058, Switzerland
REFERENCE   3  (bases 1 to 7782)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7782)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem. 
            Biol."; date: "2018"; volume: "14"; issue: "7"; pages: "723-729"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-NOV-2017) D-BSSE, ETH Zurich, Mattenstrasse 26, Basel 4058, 
            Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..215
                     /label=Psv40
                     /note="Psv40"
     promoter        18..214
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      65..200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      240..249
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    246..308
                     /label=igk secretion signal
                     /note="igk secretion signal"
     misc_feature    315..1073
                     /label=8g8f5
                     /note="8g8f5"
     misc_feature    315..647
                     /label=light
                     /note="light"
     misc_feature    708..1073
                     /label=heavy
                     /note="heavy"
     misc_feature    1086..1829
                     /label=mouse EpoR-D1_D2_TM
                     /note="mouse EpoR-D1_D2_TM"
     misc_feature    1359..1361
                     /label=F93A
                     /note="F93A"
     misc_feature    1761..1829
                     /label=transmembrane
                     /note="transmembrane"
     misc_feature    1830..3101
                     /label=mFGFR 405-end
                     /note="mFGFR 405-end"
     polyA_signal    3155..3379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3425..3853
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3867..4196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             4263..5054
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    5231..5364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5401..5417)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5425..5441)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5449..5479)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5494..5515)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5803..6388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6562..7419)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7420..7524)
                     /label=AmpR promoter
ORIGIN
        1 acgcgtgatc tgcgatctgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
       61 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatc gctgactaat
      121 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
      181 aggaggcttt tttggaggcc taggcttttg caaaaagctt cgaatcgcgc tagcggccgg
      241 ccaccatgga aactgatact ttgctgctct gggtcctgct gctgtgggtc cctggatcaa
      301 cgggggacgg atccgacatt gtcctgaccc aaagcccagc gtcgttagcg gtgtccctgg
      361 ggcaacgtgc tactatttcc tgcaaagcct cccaatctgt tgatttcgat ggggactcat
      421 acatgaactg gtatcagcaa aagcctggac agccgcctaa attacttatt ttcgccgcta
      481 gtaatctggc tagtggcatt cctgctcgcc tgtcaggttc aggttcgggc acggatttca
      541 cgctgaatat ccagcccgtt gaggaagaag atgcagccac atactactgc caacagtcca
      601 atgaagatcc ctacactttc ggggggggca ctaagttgga gatcaagggt ggtggaggat
      661 caggtggtgg agggtctggc ggaggcggta gcggtggagg tggaagccaa gtgcagcttc
      721 agcagagcgg agatgattta gtcaagccag gcgcaagtgt taaattatcc tgtaaagcat
      781 caggttacac attcactact tattacatca actggatgcg ccagcgccca ggacaaggat
      841 tagagtggat cggtcgtatt gctccagctt caggcacaac ttactcctca gagatgttca
      901 aggataaggc aacgttgact gttgatacat cttcgaatac agcgtacatt cagcttagct
      961 cgttgagttc tgaagatagt gccgtctatt tctgtgctcg cgctgattac ggctttaata
     1021 gcggggaggc tatggattat tggggacaag gtacttctgt cacggtgagt tcgtccggag
     1081 aattcgcacc ttcacccagc ctcccggacc ccaagtttga gagcaaagcg gccctgctgg
     1141 catcccgggg ctccgaagaa cttctgtgct tcacccaacg cttggaagac ttggtgtgtt
     1201 tctgggagga agcggcgagc tccgggatgg acttcaacta cagcttctca taccagctcg
     1261 agggtgagtc acgaaagtca tgtagcctgc accaggctcc caccgtccgc ggctccgtgc
     1321 gtttctggtg ttcactgcca acagcggaca catcgagtgc tgtgccgctg gagctgcagg
     1381 tgacggaggc gtccggttct cctcgctatc accgcatcat ccatatcaat gaagtagtgc
     1441 tcctggacgc ccccgcgggg ctgctggcgc gccgggcaga agagggcagc cacgtggtgc
     1501 tgcgctggct gccacctcct ggagcaccta tgaccaccca catccgatat gaagtggacg
     1561 tgtcggcagg caaccgggca ggagggacac aaagggtgga ggtcctggaa ggccgcactg
     1621 agtgtgttct gagcaacctg cggggcggga cgcgctacac cttcgctgtt cgagcgcgca
     1681 tggccgagcc gagcttcagc ggattctgga gtgcctggtc tgagcccgcg tcactactga
     1741 ccgctagcga cctggaccct ctcatcttga cgctgtctct cattctggtc ctcatctcgt
     1801 tgttgctgac ggttctggcc ctgctgtcca tgaagagcgg caccaagaag agcgacttcc
     1861 atagccagat ggctgtgcac aagctggcca agagcatccc tctgcgcaga caggtaacag
     1921 tgtcagctga ctccagtgca tccatgaact ctggggttct cctggttcgg ccctcacggc
     1981 tctcctccag cgggaccccc atgctggctg gagtctccga atatgagctc cctgaggatc
     2041 cccgctggga gctgccacga gacagactgg tcttaggcaa accacttggc gagggctgct
     2101 tcgggcaggt ggtgttggct gaggccatcg ggctggataa ggacaaaccc aaccgtgtga
     2161 ccaaagtggc cgtgaagatg ttgaagtccg acgcaacgga gaaggacctg tcggatctga
     2221 tctcggagat ggagatgatg aaaatgattg ggaagcacaa gaatatcatc aaccttctgg
     2281 gagcgtgcac acaggatggt cctctttatg tcattgtgga gtacgcctcc aaaggcaatc
     2341 tccgggagta tctacaggcc cggaggcctc ctgggctgga gtactgctat aaccccagcc
     2401 acaaccccga ggaacagctg tcttccaaag atctggtatc ctgtgcctat caggtggctc
     2461 ggggcatgga gtatcttgcc tctaagaagt gtatacaccg agacctggct gctaggaacg
     2521 tcctggtgac cgaggataac gtaatgaaga tcgcagactt tggcttagct cgagacattc
     2581 atcatatcga ctactacaag aaaaccacca acggccggct gcctgtgaag tggatggccc
     2641 ctgaggcgtt gtttgaccgg atctacacac accagagcga tgtgtggtct tttggagtgc
     2701 tcttgtggga gatcttcact ctgggtggct ccccataccc cggtgtgcct gtggaggaac
     2761 ttttcaagct gctgaaggag ggtcatcgaa tggacaagcc cagtaactgt accaatgagc
     2821 tgtacatgat gatgcgggac tgctggcatg cagtgccctc tcagagacct acgttcaagc
     2881 agttggtgga agacctggac cgcattgtgg ccttgacctc caaccaggag tatctggacc
     2941 tgtccatacc gctggaccag tactcaccca gctttcccga cacacggagc tccacctgct
     3001 cctcagggga ggactctgtc ttctctcatg agccgttacc tgaggagccc tgtctgcctc
     3061 gacaccccac ccagcttgcc aacagtggac tcaaacggcg ctaggcggcc gctcgagtct
     3121 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3181 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3241 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3301 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3361 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     3421 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3481 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3541 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3601 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3661 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3721 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     3781 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     3841 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3901 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3961 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4021 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4081 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     4141 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     4201 tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc
     4261 gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
     4321 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
     4381 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
     4441 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
     4501 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
     4561 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
     4621 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
     4681 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
     4741 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
     4801 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
     4861 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
     4921 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
     4981 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
     5041 ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa
     5101 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     5161 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     5221 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5281 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5341 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     5401 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5461 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5521 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5581 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5641 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5701 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5761 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt gcaagcagca
     6361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     6421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     6481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     6541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     6601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     6661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     6721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     6781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     6841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     6901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     6961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     7021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     7081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     7141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     7201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     7261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     7321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     7381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     7441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     7501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
     7561 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
     7621 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
     7681 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
     7741 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat at
//
