LOCUS       V006878                10490 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V006878
VERSION     V006878
KEYWORDS    pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10490)
  TITLE     A Surgical Orthotopic Approach to Study the Invasive Progression of
            Human Bladder Cancer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10490)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 10490)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..304
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    510..528
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    531..549
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    590..614
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             635..1351
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             1358..3007
                     /label="luciferase"
                     /note="firefly luciferase"
     protein_bind    complement(3024..3048)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3101..3142
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label="V5 tag"
                     /translation="GKPIPNPLLGLDST"
     promoter        3202..3712
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             3734..4330
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    4401..4989
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5069..5302
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5374..5508
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5535..5670
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5691..5709)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5719..5736)
                     /label="M13 Forward"
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21)
                     Forward"
     primer_bind     complement(5719..5735)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(5728..5750)
                     /label="M13/pUC Forward"
                     /note="In lacZ gene"
     rep_origin      5877..6332
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6358..6462
                     /label="AmpR promoter"
     CDS             6463..7320
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7494..8082
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     8236..8253
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    8370..8391
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8406..8436
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8444..8460
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8449..8471
                     /label="M13/pUC Reverse"
                     /note="In lacZ gene"
     primer_bind     8468..8484
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     8468..8484
                     /label="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     promoter        8505..8523
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8551..8777
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8778..8958
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9005..9130
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    9623..9856
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             10041..10085
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             10234..10275
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    10352..10469
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
      541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
      601 caaaaaagca ggctccgcgg ccgccccctt caccatggtg agcaagggcg aggagctgtt
      661 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      721 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      781 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      841 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      901 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      961 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     1021 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     1081 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     1141 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     1201 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag
     1261 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     1321 gatcactctc ggcatggacg agctgtacaa gtccgggatg gaagacgcca aaaacataaa
     1381 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
     1441 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     1501 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
     1561 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     1621 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     1681 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     1741 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
     1801 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     1861 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
     1921 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     1981 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     2041 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     2101 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     2161 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     2221 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     2281 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     2341 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     2401 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     2461 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     2521 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     2581 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     2641 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     2701 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     2761 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     2821 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     2881 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     2941 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     3001 cgccgtgaag ggtgggcgcg ccgacccagc tttcttgtac aaagtggttg atatccagca
     3061 cagtggcggc cgctcgagtc tagagggccc gcggttcgaa ggtaagccta tccctaaccc
     3121 tctcctcggt ctcgattcta cgcgtaccgg ttagtaatga gtttggaatt aattctgtgg
     3181 aatgtgtgtc agttagtcga gggggttggg gttgcgcctt ttccaaggca gccctgggtt
     3241 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     3301 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     3361 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     3421 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     3481 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     3541 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     3601 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     3661 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc
     3721 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     3781 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     3841 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     3901 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     3961 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     4021 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     4081 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     4141 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     4201 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     4261 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     4321 gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga gcgcacgacc
     4381 ccatgcatcg gtacgtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg
     4441 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4501 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4561 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4621 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4681 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     4741 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     4801 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     4861 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     4921 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     4981 cctccccgcc tggcgatggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     5041 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5101 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5161 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5221 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5281 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     5341 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     5401 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5461 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     5521 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     5581 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     5641 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     5701 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     5761 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     5821 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     5881 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5941 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6001 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6061 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6121 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6181 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6241 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6301 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     6361 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6421 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6481 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6541 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6601 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6661 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6721 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6781 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6841 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6901 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6961 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     7021 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     7081 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     7141 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     7201 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     7261 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     7321 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     7381 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7441 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7501 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7561 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7621 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     7681 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7741 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7801 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     7861 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     7921 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     7981 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8041 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8101 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8161 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8221 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     8281 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     8341 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     8401 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     8461 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     8521 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     8581 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     8641 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     8701 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     8761 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8821 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8881 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8941 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     9001 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     9061 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     9121 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     9181 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     9241 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     9301 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     9361 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     9421 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     9481 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     9541 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     9601 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     9661 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     9721 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     9781 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     9841 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     9901 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     9961 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
    10021 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
    10081 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
    10141 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
    10201 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
    10261 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
    10321 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt
    10381 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
    10441 aaaaacaaat tacaaaaatt caaaatttta tcgataagct tgggagttcc
//
