LOCUS       V007165                 8332 bp    DNA     circular SYN 18-DEC-2021
DEFINITION  Exported.
ACCESSION   V007165
VERSION     V007165
KEYWORDS    pLenti CMV GFP Blast (659-1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8332)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO,
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
   PUBMED   19657394
REFERENCE   2  (bases 1 to 8332)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8332)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1073..1306
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1491..1535
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1684..1725
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1802..1918
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1941..2244
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2245..2448
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     2445..2469
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     CDS             2544..3260
                     /label="EGFP"
                     /note="enhanced GFP"
     misc_feature    3279..3867
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        3923..4252
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4300..4347
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4366..4761
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             4851..5084
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5156..5290
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5317..5452
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5473..5491)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5501..5517)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5659..6114
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6140..6244
                     /label="AmpR promoter"
     CDS             6245..7102
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7276..7864
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     8018..8035
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    8152..8173
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8188..8218
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8226..8242
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8250..8266
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8287..8305
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
     1921 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1981 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2281 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2341 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2401 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
     2461 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgactc
     2521 tagaggatcc accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg
     2581 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg
     2641 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca
     2701 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     2761 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     2821 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg
     2881 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     2941 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata
     3001 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg
     3061 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
     3121 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca
     3181 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg
     3241 gcatggacga gctgtacaag taaagcggcc gcgtcgacaa tcaacctctg gattacaaaa
     3301 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3361 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3421 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3481 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3541 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3601 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3661 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     3721 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3781 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3841 ggatctccct ttgggccgcc tccccgcctg gaattctgca gatatccggt tagtaatgag
     3901 tttggaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4021 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4081 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4141 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4201 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4261 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     4321 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagcctttg
     4381 tctcaagaag aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc
     4441 tctgaagact acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt
     4501 gtcaatgtat atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct
     4561 gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc
     4621 atcttgagcc cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa
     4681 gccatagtga aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc
     4741 tctggttatg tgtgggaggg ctaagcacaa ttcgagctcg gtacctttaa gaccaatgac
     4801 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4861 agctcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     4921 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4981 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5041 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     5101 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     5161 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5221 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5281 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     5341 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     5401 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     5461 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     5521 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     5581 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     5641 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     5701 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     5761 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     5821 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     5881 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     5941 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     6001 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     6061 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     6121 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6181 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6241 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6301 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6361 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6421 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6481 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6541 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6601 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6661 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6721 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6781 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6841 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6901 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6961 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7021 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7081 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7141 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7201 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7261 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7321 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7381 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7441 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7501 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7561 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7621 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7681 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7741 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7801 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7861 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7921 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7981 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8041 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8101 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     8161 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     8221 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     8281 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aa
//
