LOCUS       V001184                 9388 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001184
VERSION     V001184
KEYWORDS    pLenti CMV/TO SV40 small + Large T (w612-1)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9388)
  TITLE     shMDC1 plasmids
REFERENCE   2  (bases 1 to 9388)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 9388)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        21..324
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        325..528
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    530..548
                     /label="tet operator"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    551..569
                     /gene="tetO"
                     /label="tet operator"
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    610..634
                     /label="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             741..1262
                     /label="small t antigen"
                     /note="SV40 (simian virus 40) small t antigen"
     CDS             1462..1482
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     protein_bind    complement(3277..3301)
                     /label="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3332..3920
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4007..4240
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4312..4446
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4452..4587
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(4608..4626)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4636..4652)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4794..5249
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5276..5380
                     /label="AmpR promoter"
     CDS             5381..6238
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6412..7000
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     7154..7171
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    7288..7309
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7324..7354
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7362..7378
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7386..7402
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7423..7441
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7469..7695
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7696..7876
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7923..8048
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    8541..8774
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             8959..9003
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             9152..9193
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    9270..9386
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
ORIGIN
        1 cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
       61 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      121 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      181 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      241 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      301 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
      361 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
      421 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
      481 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct ccctatcagt
      541 gatagagatc tccctatcag tgatagagat cgtcgactag tccagtgtgg tggaattctg
      601 cagatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgactgg
      661 atccgcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
      721 ttgcaaaaag ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
      781 tggaccttct aggtcttgaa aggagtgcct gggggaatat tcctctgatg agaaaggcat
      841 atttaaaaaa atgcaaggag tttcatcctg ataaaggagg agatgaagaa aaaatgaaga
      901 aaatgaatac tctgtacaag aaaatggaag atggagtaaa atatgctcat caacctgact
      961 ttggaggctt ctgggatgca actgaggtat ttgcttcttc cttaaatcct ggtgttgatg
     1021 caatgtactg caaacaatgg cctgagtgtg caaagaaaat gtctgctaac tgcatatgct
     1081 tgctgtgctt actgaggatg aagcatgaaa atagaaaatt atacaggaaa gatccacttg
     1141 tgtgggttga ttgctactgc ttcgattgct ttagaatgtg gtttggactt gatctttgtg
     1201 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     1261 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     1321 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     1381 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     1441 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     1501 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     1561 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     1621 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     1681 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     1741 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     1801 gatccatttt ctgttattga ggaaagtttg ccaggtgggt taaaggagca tgattttaat
     1861 ccagaagaag cagaggaaac taaacaagtg tcctggaagc ttgtaacaga gtatgcaatg
     1921 gaaacaaaat gtgatgatgt gttgttattg cttgggatgt acttggaatt tcagtacagt
     1981 tttgaaatgt gtttaaaatg tattaaaaaa gaacagccca gccactataa gtaccatgaa
     2041 aagcattatg caaatgctgc tatatttgct gacagcaaaa accaaaaaac catatgccaa
     2101 caggctgttg atactgtttt agctaaaaag cgggttgata gcctacaatt aactagagaa
     2161 caaatgttaa caaacagatt taatgatctt ttggatagga tggatataat gtttggttct
     2221 acaggctctg ctgacataga agaatggatg gctggagttg cttggctaca ctgtttgttg
     2281 cccaaaatgg attcagtggt gtatgacttt ttaaaatgca tggtgtacaa cattcctaaa
     2341 aaaagatact ggctgtttaa aggaccaatt gatagtggta aaactacatt agcagctgct
     2401 ttgcttgaat tatgtggggg gaaagcttta aatgttaatt tgcccttgga caggctgaac
     2461 tttgagctag gagtagctat tgaccagttt ttagtagttt ttgaggatgt aaagggcact
     2521 ggaggggagt ccagagattt gccttcaggt cagggaatta ataacctgga caatttaagg
     2581 gattatttgg atggcagtgt taaggtaaac ttagaaaaga aacacctaaa taaaagaact
     2641 caaatatttc cccctggaat agtcaccatg aatgagtaca gtgtgcctaa aacactgcag
     2701 gccagatttg taaaacaaat agattttagg cccaaagatt atttaaagca ttgcctggaa
     2761 cgcagtgagt ttttgttaga aaagagaata attcaaagtg gcattgcttt gcttcttatg
     2821 ttaatttggt acagacctgt ggctgagttt gctcaaagta ttcagagcag aattgtggag
     2881 tggaaagaga gattggacaa agagtttagt ttgtcagtgt atcaaaaaat gaagtttaat
     2941 gtggctatgg gaattggagt tttagattgg ctaagaaaca gtgatgatga tgatgaagac
     3001 agccaggaaa atgctgataa aaatgaagat ggtggggaga agaacatgga agactcaggg
     3061 catgaaacag gcattgattc acagtcccaa ggctcatttc aggcccctca gtcctcacag
     3121 tctgttcatg atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     3181 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttggatc
     3241 cggtaccgaa ttcgcggccg cactcgagat atctagaccc agctttcttg tacaaagtgg
     3301 ttgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga
     3361 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3421 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3481 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3541 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3601 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3661 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3721 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3781 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     3841 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     3901 cctttgggcc gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac
     3961 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctagct
     4021 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     4081 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     4141 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     4201 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     4261 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     4321 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4381 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4441 tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4501 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     4561 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     4621 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4681 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4741 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4801 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4861 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4921 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4981 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     5041 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     5101 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     5161 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     5221 ttttaacaaa atattaacgc ttacaatttt aggtggcact tttcggggaa atgtgcgcgg
     5281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5341 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5401 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5461 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5521 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5581 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5641 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5701 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5761 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5821 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5881 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5941 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6001 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6061 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6121 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6181 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6241 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6301 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6361 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6421 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6481 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6541 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6601 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6661 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6721 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6781 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     6841 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6901 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6961 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7021 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7081 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7141 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7201 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     7261 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     7321 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     7381 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     7441 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     7501 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     7561 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     7621 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     7681 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7741 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7801 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7861 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     7921 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     7981 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8041 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     8101 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     8161 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     8221 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     8281 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     8341 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     8401 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     8461 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     8521 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     8581 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     8641 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     8701 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     8761 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     8821 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     8881 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     8941 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9001 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9061 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     9121 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     9181 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     9241 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
     9301 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     9361 aaacaaatta caaaattcaa aattttat
//
