LOCUS       V011773                 9630 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011773
VERSION     V011773
KEYWORDS    pLenti7.3 V5-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1804..1921
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        1944..2247
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2248..2451
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    2568..2692
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2717..2747
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2801..3457
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             3802..4104
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4148..4272)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4325..4366
                     /label="V5 tag"
                     /note="epitope tag from simian virus 5"
     misc_feature    4390..4978
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     promoter        4993..5309
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             5360..6076
                     /label="EmGFP"
                     /note="Emerald GFP"
     LTR             6151..6384
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6456..6590
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6617..6752
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6773..6791)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6801..6817)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6959..7414
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7440..7544
                     /label="AmpR promoter"
     CDS             7545..8402
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8576..9164
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9452..9473
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9488..9518
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9526..9542
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9550..9566
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9587..9605
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     1981 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2041 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2101 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2161 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2221 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2281 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2341 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2401 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2461 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccga
     2521 ctctagagga tccactagtc cagtgtggtg gaattctgca gatatcaaca agtttgtaca
     2581 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc
     2641 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc
     2701 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag
     2761 ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     2821 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     2881 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     2941 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     3001 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag
     3061 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     3121 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     3181 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     3241 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     3301 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     3361 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga
     3421 attacaacag tactgcgatg agtggcaggg cggggcgtaa agatctggat ccggcttact
     3481 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac
     3541 tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg
     3601 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc
     3661 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg
     3721 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg
     3781 acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt
     3841 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg
     3901 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg
     3961 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc
     4021 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc
     4081 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca
     4141 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg
     4201 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt
     4261 gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     4321 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta
     4381 atgatcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4441 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4501 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4561 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4621 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4681 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4741 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4801 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4861 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4921 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4981 ggcgatggta ccggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5041 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     5101 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     5161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     5221 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5281 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5341 gatctgatca gcacctagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5401 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5461 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5521 gcccgtgccc tggcccaccc tcgtgaccac cttcacctac ggcgtgcagt gcttcgcccg
     5581 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5641 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5701 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5761 cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg tctatatcac
     5821 cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga
     5881 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5941 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     6001 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     6061 ggacgagctg tacaagtaag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     6121 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6181 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6241 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6301 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6361 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6421 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6481 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6541 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     6601 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6661 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6721 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     6781 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6841 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6901 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6961 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     7021 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     7081 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     7141 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     7201 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     7261 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     7321 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     7381 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7441 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7501 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7561 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7621 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7681 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7741 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7801 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7861 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7921 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7981 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     8041 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     8101 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     8161 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     8221 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     8281 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     8341 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     8401 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8461 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8701 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9241 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9301 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     9361 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9421 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9481 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9541 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     9601 aaagggaaca aaagctggag ctgcaagctt
//
